TGF-[beta]2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing
Background Mesenchymal stem cells (MSCs) secrete paracrine factors and extracellular matrix proteins that contribute to their ability to support tissue healing and regeneration. Both the transcriptome and the secretome of MSCs can be altered by treating the cells with cytokines, but neither have bee...
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| Veröffentlicht in: | Stem cell research & therapy 2022-09, Vol.13 (1) |
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| creator | Koch, Drew W Schnabel, Lauren V Ellis, Ilene M Bates, Rowan E Berglund, Alix K |
| description | Background Mesenchymal stem cells (MSCs) secrete paracrine factors and extracellular matrix proteins that contribute to their ability to support tissue healing and regeneration. Both the transcriptome and the secretome of MSCs can be altered by treating the cells with cytokines, but neither have been thoroughly investigated following treatment with the specific cytokine transforming growth factor (TGF)-[beta]2. Methods RNA-sequencing and western blotting were used to compare gene and protein expression between untreated and TGF-[beta]2-treated equine bone marrow-derived MSCs (BM-MSCs). A co-culture system was utilized to compare equine tenocyte migration during co-culture with untreated and TGF-[beta]2-treated BM-MSCs. Results TGF-[beta]2 treatment significantly upregulated gene expression of collagens, extracellular matrix molecules, and growth factors. Protein expression of collagen type I and tenascin-C was also confirmed to be upregulated in TGF-[beta]2-treated BM-MSCs compared to untreated BM-MSCs. Both untreated and TGF-[beta]2-treated BM-MSCs increased tenocyte migration in vitro. Conclusions Treating equine BM-MSCs with TGF-[beta]2 significantly increases production of paracrine factors and extracellular matrix molecules important for tendon healing and promotes the migration of tenocytes in vitro. Keywords: Mesenchymal stem cell, Tenocyte, TGF-[beta], Equine, Tendon, RNA-sequencing, Paracrine factors, Extracellular matrix |
| doi_str_mv | 10.1186/s13287-022-03172-9 |
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Both the transcriptome and the secretome of MSCs can be altered by treating the cells with cytokines, but neither have been thoroughly investigated following treatment with the specific cytokine transforming growth factor (TGF)-[beta]2. Methods RNA-sequencing and western blotting were used to compare gene and protein expression between untreated and TGF-[beta]2-treated equine bone marrow-derived MSCs (BM-MSCs). A co-culture system was utilized to compare equine tenocyte migration during co-culture with untreated and TGF-[beta]2-treated BM-MSCs. Results TGF-[beta]2 treatment significantly upregulated gene expression of collagens, extracellular matrix molecules, and growth factors. Protein expression of collagen type I and tenascin-C was also confirmed to be upregulated in TGF-[beta]2-treated BM-MSCs compared to untreated BM-MSCs. Both untreated and TGF-[beta]2-treated BM-MSCs increased tenocyte migration in vitro. Conclusions Treating equine BM-MSCs with TGF-[beta]2 significantly increases production of paracrine factors and extracellular matrix molecules important for tendon healing and promotes the migration of tenocytes in vitro. Keywords: Mesenchymal stem cell, Tenocyte, TGF-[beta], Equine, Tendon, RNA-sequencing, Paracrine factors, Extracellular matrix</description><identifier>ISSN: 1757-6512</identifier><identifier>EISSN: 1757-6512</identifier><identifier>DOI: 10.1186/s13287-022-03172-9</identifier><language>eng</language><publisher>BioMed Central Ltd</publisher><subject>Collagen ; Cytokines ; Gene expression ; Genes ; Stem cells ; Transforming growth factors</subject><ispartof>Stem cell research & therapy, 2022-09, Vol.13 (1)</ispartof><rights>COPYRIGHT 2022 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Koch, Drew W</creatorcontrib><creatorcontrib>Schnabel, Lauren V</creatorcontrib><creatorcontrib>Ellis, Ilene M</creatorcontrib><creatorcontrib>Bates, Rowan E</creatorcontrib><creatorcontrib>Berglund, Alix K</creatorcontrib><title>TGF-[beta]2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing</title><title>Stem cell research & therapy</title><description>Background Mesenchymal stem cells (MSCs) secrete paracrine factors and extracellular matrix proteins that contribute to their ability to support tissue healing and regeneration. Both the transcriptome and the secretome of MSCs can be altered by treating the cells with cytokines, but neither have been thoroughly investigated following treatment with the specific cytokine transforming growth factor (TGF)-[beta]2. Methods RNA-sequencing and western blotting were used to compare gene and protein expression between untreated and TGF-[beta]2-treated equine bone marrow-derived MSCs (BM-MSCs). A co-culture system was utilized to compare equine tenocyte migration during co-culture with untreated and TGF-[beta]2-treated BM-MSCs. Results TGF-[beta]2 treatment significantly upregulated gene expression of collagens, extracellular matrix molecules, and growth factors. Protein expression of collagen type I and tenascin-C was also confirmed to be upregulated in TGF-[beta]2-treated BM-MSCs compared to untreated BM-MSCs. Both untreated and TGF-[beta]2-treated BM-MSCs increased tenocyte migration in vitro. Conclusions Treating equine BM-MSCs with TGF-[beta]2 significantly increases production of paracrine factors and extracellular matrix molecules important for tendon healing and promotes the migration of tenocytes in vitro. Keywords: Mesenchymal stem cell, Tenocyte, TGF-[beta], Equine, Tendon, RNA-sequencing, Paracrine factors, Extracellular matrix</description><subject>Collagen</subject><subject>Cytokines</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Stem cells</subject><subject>Transforming growth factors</subject><issn>1757-6512</issn><issn>1757-6512</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNptkMlKBDEQhhtRUNQX8BQQBA_RJL3mKKKjIAguJ5GhOl09He1OtCvj6FP4ysblMAMmkArF99fyJ8meFEdSVsUxyVRVJRdKcZHKUnG9lmzJMi95kUu1vvTfTHaJnkQ8aSpEkW0ln3eTc_5QY4BHxdB14AwSw_eXEYmsd8y3DF_n1iGrfXwGGEe_4A2O9g0bNiChM93HAD2jgAMz2PfsBUYw47emBRP8SGxhQ8eenV84BkTeWAixOLHgWUDXxD4dQm_dbCfZaKEn3P2L28n9-dnd6QW_up5cnp5c8VlcNuNaZ2XaaMxB6VKkaFArpaGtZUzKItqQ11VdQa5SFFDLulUV5hrrymSNLpp0O9n_rTuDHqfWtT7EmQdLZnpSykqoTOosUkf_UPE2OFgT_WhtzK8IDlcEkQn4HmYwJ5pe3t6ssgdL7Pf-oSPfz3-cWQa_AEwAlD4</recordid><startdate>20220916</startdate><enddate>20220916</enddate><creator>Koch, Drew W</creator><creator>Schnabel, Lauren V</creator><creator>Ellis, Ilene M</creator><creator>Bates, Rowan E</creator><creator>Berglund, Alix K</creator><general>BioMed Central Ltd</general><scope>ISR</scope></search><sort><creationdate>20220916</creationdate><title>TGF-[beta]2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing</title><author>Koch, Drew W ; Schnabel, Lauren V ; Ellis, Ilene M ; Bates, Rowan E ; Berglund, Alix K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1324-99473d9e5a29703ece9229afb1d9e160315b8b8a523e0ab1bf28e59eb8c4d96d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Collagen</topic><topic>Cytokines</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Stem cells</topic><topic>Transforming growth factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koch, Drew W</creatorcontrib><creatorcontrib>Schnabel, Lauren V</creatorcontrib><creatorcontrib>Ellis, Ilene M</creatorcontrib><creatorcontrib>Bates, Rowan E</creatorcontrib><creatorcontrib>Berglund, Alix K</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>Stem cell research & therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koch, Drew W</au><au>Schnabel, Lauren V</au><au>Ellis, Ilene M</au><au>Bates, Rowan E</au><au>Berglund, Alix K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TGF-[beta]2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing</atitle><jtitle>Stem cell research & therapy</jtitle><date>2022-09-16</date><risdate>2022</risdate><volume>13</volume><issue>1</issue><issn>1757-6512</issn><eissn>1757-6512</eissn><abstract>Background Mesenchymal stem cells (MSCs) secrete paracrine factors and extracellular matrix proteins that contribute to their ability to support tissue healing and regeneration. Both the transcriptome and the secretome of MSCs can be altered by treating the cells with cytokines, but neither have been thoroughly investigated following treatment with the specific cytokine transforming growth factor (TGF)-[beta]2. Methods RNA-sequencing and western blotting were used to compare gene and protein expression between untreated and TGF-[beta]2-treated equine bone marrow-derived MSCs (BM-MSCs). A co-culture system was utilized to compare equine tenocyte migration during co-culture with untreated and TGF-[beta]2-treated BM-MSCs. Results TGF-[beta]2 treatment significantly upregulated gene expression of collagens, extracellular matrix molecules, and growth factors. Protein expression of collagen type I and tenascin-C was also confirmed to be upregulated in TGF-[beta]2-treated BM-MSCs compared to untreated BM-MSCs. Both untreated and TGF-[beta]2-treated BM-MSCs increased tenocyte migration in vitro. Conclusions Treating equine BM-MSCs with TGF-[beta]2 significantly increases production of paracrine factors and extracellular matrix molecules important for tendon healing and promotes the migration of tenocytes in vitro. Keywords: Mesenchymal stem cell, Tenocyte, TGF-[beta], Equine, Tendon, RNA-sequencing, Paracrine factors, Extracellular matrix</abstract><pub>BioMed Central Ltd</pub><doi>10.1186/s13287-022-03172-9</doi></addata></record> |
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| source | Springer Nature - Complete Springer Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access; Springer Nature OA Free Journals |
| subjects | Collagen Cytokines Gene expression Genes Stem cells Transforming growth factors |
| title | TGF-[beta]2 enhances expression of equine bone marrow-derived mesenchymal stem cell paracrine factors with known associations to tendon healing |
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