Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors
Purpose The quantifiable description of PPAR[gamma] expression pattern beside mechanistic in-vitro evidence will provide insights into the involvement of this mediator in tumor pathogenesis. This study is focused on illuminating the PPAR[gamma] gene and protein expression pattern, its association wi...
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| Veröffentlicht in: | BMC musculoskeletal disorders 2022-08, Vol.23 (1) |
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| container_title | BMC musculoskeletal disorders |
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| creator | Eghtedari, Amir Reza Vaezi, Mohammad Amin Safizadeh, Banafsheh Ghasempour, Ghasem Babaheidarian, Pegah Salimi, Vahid Tavakoli-Yaraki, Masoumeh |
| description | Purpose The quantifiable description of PPAR[gamma] expression pattern beside mechanistic in-vitro evidence will provide insights into the involvement of this mediator in tumor pathogenesis. This study is focused on illuminating the PPAR[gamma] gene and protein expression pattern, its association with tumor deterioration and its diagnostic value in different types of primary bone tumors. Methods The expression pattern of PPAR[gamma] was investigated in the 180 bone tissues including 90 bone tumor tissues and 90 non-cancerous bone tissues. The local PPAR[gamma] expression level was assessed using real-time qRT-PCR and the PPAR[gamma] protein expression pattern was measured using immunohistochemistry. The correlation of PPAR[gamma] expression level with patients' clinic-pathological features, also the value of the variables in predicting PPAR[gamma] expression level in tumors and the value of PPAR[gamma] to discriminate tumor subtypes were assessed. Results The mean PPAR[gamma] mRNA expression was significantly higher in bone tumors compared to healthy bone tissues, also the malignant tumors including osteosarcoma and Ewing sarcoma had the elevated level of PPAR[gamma] mRNA compared to GCT tumors. Consistently, the protein expression of PPAR[gamma] in the tumor site was significantly higher in the bone tumors and malignant tumors compared to non-cancerous and benign tumors, respectively. The PPAR[gamma] protein could predict malignant tumor features including tumor grade, metastasis and recurrence significantly. Moreover, PPAR[gamma] could potentially discriminate the patients from the controls also malignant tumors from benign tumors with significant sensitivity and specificity. Conclusions PPAR[gamma] might be involved in primary bone tumor pathogenesis and determining its molecular mechanism regarding bone cancer pathogenesis is of grave importance. Keywords: PPAR[gamma], Bone tumor, Osteosarcoma, Ewing sarcoma, GCT |
| doi_str_mv | 10.1186/s12891-022-05681-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A712510815</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A712510815</galeid><sourcerecordid>A712510815</sourcerecordid><originalsourceid>FETCH-LOGICAL-g675-b191f2625f2543c08ba4e45f1035346f127f3d2f79e3bde952e3fbcd99fc56e83</originalsourceid><addsrcrecordid>eNptjk1LAzEQhoMoWKt_wFPA89ZMstnsHkupH1CwSG8iJZudrJFNtmxS0X_vVj30IHOYmZfnGYaQa2AzgLK4jcDLCjLGecZkUUImTsgEcgUZz1V-ejSfk4sY3xkDVYpqQtLyQ3d7nVwfaG9pekOKn7sBYzwkO50SDoHq0NDG6Tb0MTlDDwoe8PV6_vzSau_1K3WBet25NuiQfoQaw7jR3eC8Hr5o3Qekae_7IV6SM6u7iFd_fUo2d8vN4iFbPd0_LuarrC2UzGqowPKCS8tlLgwra51jLi0wIUVeWODKioZbVaGoG6wkR2Fr01SVNbLAUkzJze_ZVne4dcH2adDGu2i2cwVcAitBjtTsH2qsBr0z49PWjfmR8A27gG4m</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Springer Nature - Complete Springer Journals</source><source>PubMed Central</source><source>Springer Nature OA/Free Journals</source><creator>Eghtedari, Amir Reza ; Vaezi, Mohammad Amin ; Safizadeh, Banafsheh ; Ghasempour, Ghasem ; Babaheidarian, Pegah ; Salimi, Vahid ; Tavakoli-Yaraki, Masoumeh</creator><creatorcontrib>Eghtedari, Amir Reza ; Vaezi, Mohammad Amin ; Safizadeh, Banafsheh ; Ghasempour, Ghasem ; Babaheidarian, Pegah ; Salimi, Vahid ; Tavakoli-Yaraki, Masoumeh</creatorcontrib><description>Purpose The quantifiable description of PPAR[gamma] expression pattern beside mechanistic in-vitro evidence will provide insights into the involvement of this mediator in tumor pathogenesis. This study is focused on illuminating the PPAR[gamma] gene and protein expression pattern, its association with tumor deterioration and its diagnostic value in different types of primary bone tumors. Methods The expression pattern of PPAR[gamma] was investigated in the 180 bone tissues including 90 bone tumor tissues and 90 non-cancerous bone tissues. The local PPAR[gamma] expression level was assessed using real-time qRT-PCR and the PPAR[gamma] protein expression pattern was measured using immunohistochemistry. The correlation of PPAR[gamma] expression level with patients' clinic-pathological features, also the value of the variables in predicting PPAR[gamma] expression level in tumors and the value of PPAR[gamma] to discriminate tumor subtypes were assessed. Results The mean PPAR[gamma] mRNA expression was significantly higher in bone tumors compared to healthy bone tissues, also the malignant tumors including osteosarcoma and Ewing sarcoma had the elevated level of PPAR[gamma] mRNA compared to GCT tumors. Consistently, the protein expression of PPAR[gamma] in the tumor site was significantly higher in the bone tumors and malignant tumors compared to non-cancerous and benign tumors, respectively. The PPAR[gamma] protein could predict malignant tumor features including tumor grade, metastasis and recurrence significantly. Moreover, PPAR[gamma] could potentially discriminate the patients from the controls also malignant tumors from benign tumors with significant sensitivity and specificity. Conclusions PPAR[gamma] might be involved in primary bone tumor pathogenesis and determining its molecular mechanism regarding bone cancer pathogenesis is of grave importance. Keywords: PPAR[gamma], Bone tumor, Osteosarcoma, Ewing sarcoma, GCT</description><identifier>ISSN: 1471-2474</identifier><identifier>EISSN: 1471-2474</identifier><identifier>DOI: 10.1186/s12891-022-05681-3</identifier><language>eng</language><publisher>BioMed Central Ltd</publisher><subject>Bone tumors ; Diagnosis ; Genetic aspects ; Health aspects ; Patient outcomes ; Peroxisomes ; Physiological aspects</subject><ispartof>BMC musculoskeletal disorders, 2022-08, Vol.23 (1)</ispartof><rights>COPYRIGHT 2022 BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,861,27905,27906</link.rule.ids></links><search><creatorcontrib>Eghtedari, Amir Reza</creatorcontrib><creatorcontrib>Vaezi, Mohammad Amin</creatorcontrib><creatorcontrib>Safizadeh, Banafsheh</creatorcontrib><creatorcontrib>Ghasempour, Ghasem</creatorcontrib><creatorcontrib>Babaheidarian, Pegah</creatorcontrib><creatorcontrib>Salimi, Vahid</creatorcontrib><creatorcontrib>Tavakoli-Yaraki, Masoumeh</creatorcontrib><title>Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors</title><title>BMC musculoskeletal disorders</title><description>Purpose The quantifiable description of PPAR[gamma] expression pattern beside mechanistic in-vitro evidence will provide insights into the involvement of this mediator in tumor pathogenesis. This study is focused on illuminating the PPAR[gamma] gene and protein expression pattern, its association with tumor deterioration and its diagnostic value in different types of primary bone tumors. Methods The expression pattern of PPAR[gamma] was investigated in the 180 bone tissues including 90 bone tumor tissues and 90 non-cancerous bone tissues. The local PPAR[gamma] expression level was assessed using real-time qRT-PCR and the PPAR[gamma] protein expression pattern was measured using immunohistochemistry. The correlation of PPAR[gamma] expression level with patients' clinic-pathological features, also the value of the variables in predicting PPAR[gamma] expression level in tumors and the value of PPAR[gamma] to discriminate tumor subtypes were assessed. Results The mean PPAR[gamma] mRNA expression was significantly higher in bone tumors compared to healthy bone tissues, also the malignant tumors including osteosarcoma and Ewing sarcoma had the elevated level of PPAR[gamma] mRNA compared to GCT tumors. Consistently, the protein expression of PPAR[gamma] in the tumor site was significantly higher in the bone tumors and malignant tumors compared to non-cancerous and benign tumors, respectively. The PPAR[gamma] protein could predict malignant tumor features including tumor grade, metastasis and recurrence significantly. Moreover, PPAR[gamma] could potentially discriminate the patients from the controls also malignant tumors from benign tumors with significant sensitivity and specificity. Conclusions PPAR[gamma] might be involved in primary bone tumor pathogenesis and determining its molecular mechanism regarding bone cancer pathogenesis is of grave importance. Keywords: PPAR[gamma], Bone tumor, Osteosarcoma, Ewing sarcoma, GCT</description><subject>Bone tumors</subject><subject>Diagnosis</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Patient outcomes</subject><subject>Peroxisomes</subject><subject>Physiological aspects</subject><issn>1471-2474</issn><issn>1471-2474</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjk1LAzEQhoMoWKt_wFPA89ZMstnsHkupH1CwSG8iJZudrJFNtmxS0X_vVj30IHOYmZfnGYaQa2AzgLK4jcDLCjLGecZkUUImTsgEcgUZz1V-ejSfk4sY3xkDVYpqQtLyQ3d7nVwfaG9pekOKn7sBYzwkO50SDoHq0NDG6Tb0MTlDDwoe8PV6_vzSau_1K3WBet25NuiQfoQaw7jR3eC8Hr5o3Qekae_7IV6SM6u7iFd_fUo2d8vN4iFbPd0_LuarrC2UzGqowPKCS8tlLgwra51jLi0wIUVeWODKioZbVaGoG6wkR2Fr01SVNbLAUkzJze_ZVne4dcH2adDGu2i2cwVcAitBjtTsH2qsBr0z49PWjfmR8A27gG4m</recordid><startdate>20220803</startdate><enddate>20220803</enddate><creator>Eghtedari, Amir Reza</creator><creator>Vaezi, Mohammad Amin</creator><creator>Safizadeh, Banafsheh</creator><creator>Ghasempour, Ghasem</creator><creator>Babaheidarian, Pegah</creator><creator>Salimi, Vahid</creator><creator>Tavakoli-Yaraki, Masoumeh</creator><general>BioMed Central Ltd</general><scope/></search><sort><creationdate>20220803</creationdate><title>Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors</title><author>Eghtedari, Amir Reza ; Vaezi, Mohammad Amin ; Safizadeh, Banafsheh ; Ghasempour, Ghasem ; Babaheidarian, Pegah ; Salimi, Vahid ; Tavakoli-Yaraki, Masoumeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g675-b191f2625f2543c08ba4e45f1035346f127f3d2f79e3bde952e3fbcd99fc56e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Bone tumors</topic><topic>Diagnosis</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Patient outcomes</topic><topic>Peroxisomes</topic><topic>Physiological aspects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eghtedari, Amir Reza</creatorcontrib><creatorcontrib>Vaezi, Mohammad Amin</creatorcontrib><creatorcontrib>Safizadeh, Banafsheh</creatorcontrib><creatorcontrib>Ghasempour, Ghasem</creatorcontrib><creatorcontrib>Babaheidarian, Pegah</creatorcontrib><creatorcontrib>Salimi, Vahid</creatorcontrib><creatorcontrib>Tavakoli-Yaraki, Masoumeh</creatorcontrib><jtitle>BMC musculoskeletal disorders</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eghtedari, Amir Reza</au><au>Vaezi, Mohammad Amin</au><au>Safizadeh, Banafsheh</au><au>Ghasempour, Ghasem</au><au>Babaheidarian, Pegah</au><au>Salimi, Vahid</au><au>Tavakoli-Yaraki, Masoumeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors</atitle><jtitle>BMC musculoskeletal disorders</jtitle><date>2022-08-03</date><risdate>2022</risdate><volume>23</volume><issue>1</issue><issn>1471-2474</issn><eissn>1471-2474</eissn><abstract>Purpose The quantifiable description of PPAR[gamma] expression pattern beside mechanistic in-vitro evidence will provide insights into the involvement of this mediator in tumor pathogenesis. This study is focused on illuminating the PPAR[gamma] gene and protein expression pattern, its association with tumor deterioration and its diagnostic value in different types of primary bone tumors. Methods The expression pattern of PPAR[gamma] was investigated in the 180 bone tissues including 90 bone tumor tissues and 90 non-cancerous bone tissues. The local PPAR[gamma] expression level was assessed using real-time qRT-PCR and the PPAR[gamma] protein expression pattern was measured using immunohistochemistry. The correlation of PPAR[gamma] expression level with patients' clinic-pathological features, also the value of the variables in predicting PPAR[gamma] expression level in tumors and the value of PPAR[gamma] to discriminate tumor subtypes were assessed. Results The mean PPAR[gamma] mRNA expression was significantly higher in bone tumors compared to healthy bone tissues, also the malignant tumors including osteosarcoma and Ewing sarcoma had the elevated level of PPAR[gamma] mRNA compared to GCT tumors. Consistently, the protein expression of PPAR[gamma] in the tumor site was significantly higher in the bone tumors and malignant tumors compared to non-cancerous and benign tumors, respectively. The PPAR[gamma] protein could predict malignant tumor features including tumor grade, metastasis and recurrence significantly. Moreover, PPAR[gamma] could potentially discriminate the patients from the controls also malignant tumors from benign tumors with significant sensitivity and specificity. Conclusions PPAR[gamma] might be involved in primary bone tumor pathogenesis and determining its molecular mechanism regarding bone cancer pathogenesis is of grave importance. Keywords: PPAR[gamma], Bone tumor, Osteosarcoma, Ewing sarcoma, GCT</abstract><pub>BioMed Central Ltd</pub><doi>10.1186/s12891-022-05681-3</doi></addata></record> |
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| subjects | Bone tumors Diagnosis Genetic aspects Health aspects Patient outcomes Peroxisomes Physiological aspects |
| title | Evaluation of the expression pattern and diagnostic value of PPAR[gamma] in malignant and benign primary bone tumors |
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