Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?
OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured...
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| Veröffentlicht in: | Gynecology, obstetrics & reproductive medicine : GORM obstetrics & reproductive medicine : GORM, 2021-12, Vol.27 (3), p.234-7 |
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| container_title | Gynecology, obstetrics & reproductive medicine : GORM |
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| creator | Irez, Tulay Ercan, Sinem Ciraci, Enver Aksoyer, Saadet Busra Toprak, M. Sait Dayioglu, Nurten Guralp, Onur |
| description | OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter. |
| doi_str_mv | 10.21613/GORM.2020.1087 |
| format | Article |
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Sait ; Dayioglu, Nurten ; Guralp, Onur</creator><creatorcontrib>Irez, Tulay ; Ercan, Sinem ; Ciraci, Enver ; Aksoyer, Saadet Busra ; Toprak, M. Sait ; Dayioglu, Nurten ; Guralp, Onur</creatorcontrib><description>OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.</description><identifier>ISSN: 1300-4751</identifier><identifier>EISSN: 2602-4918</identifier><identifier>DOI: 10.21613/GORM.2020.1087</identifier><language>eng</language><publisher>Medical Network</publisher><subject>Apoptosis ; Comparative analysis ; Embryonic development ; Glycoproteins ; Luteinizing hormone ; Pituitary hormones</subject><ispartof>Gynecology, obstetrics & reproductive medicine : GORM, 2021-12, Vol.27 (3), p.234-7</ispartof><rights>COPYRIGHT 2021 Medical Network</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-3517-3046 ; 0000-0001-8272-4931 ; 0000-0002-5102-6599 ; 0000-0003-4103-6045 ; 0000-0001-5726-4734 ; 0000-0001-7904-4556 ; 0000-0002-8338-9546</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids></links><search><creatorcontrib>Irez, Tulay</creatorcontrib><creatorcontrib>Ercan, Sinem</creatorcontrib><creatorcontrib>Ciraci, Enver</creatorcontrib><creatorcontrib>Aksoyer, Saadet Busra</creatorcontrib><creatorcontrib>Toprak, M. Sait</creatorcontrib><creatorcontrib>Dayioglu, Nurten</creatorcontrib><creatorcontrib>Guralp, Onur</creatorcontrib><title>Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?</title><title>Gynecology, obstetrics & reproductive medicine : GORM</title><description>OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.</description><subject>Apoptosis</subject><subject>Comparative analysis</subject><subject>Embryonic development</subject><subject>Glycoproteins</subject><subject>Luteinizing hormone</subject><subject>Pituitary hormones</subject><issn>1300-4751</issn><issn>2602-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNptkMFLwzAUxoMoOObOXgOe270kbdOeZFSdwsZkjF08lDR91UjajKY97L-327wI8g4ffPy-d_gRcs8g5CxhYr7cbNchBw4hg1RekQlPgAdRxtJrMmECIIhkzG7JzPtvAOCMRzyKJ-TjyaGn_RfSxcEdeueNp3tlB6SupvnQDHbwNEdrPX236kgV3TqL1LR0i16P2MbpY38u9qbvHF2rfuhUb1z7eEduamU9zn5zSnYvz7v8NVhtlm_5YhVoBjIJ0kShgDLRsSql1BViUmklIUorIVmFJdcos4rLWjJe1kpxAFlmSnMZiRTFlDxc3n4qi4Vpa9d3SjfG62KRZAAiFjwZqfAfarwKG6Ndi7UZ-z-D-WWgO-d9h3Vx6EyjumPBoDhLL07Si5P04iRd_AAtZnMn</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Irez, Tulay</creator><creator>Ercan, Sinem</creator><creator>Ciraci, Enver</creator><creator>Aksoyer, Saadet Busra</creator><creator>Toprak, M. Sait</creator><creator>Dayioglu, Nurten</creator><creator>Guralp, Onur</creator><general>Medical Network</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3517-3046</orcidid><orcidid>https://orcid.org/0000-0001-8272-4931</orcidid><orcidid>https://orcid.org/0000-0002-5102-6599</orcidid><orcidid>https://orcid.org/0000-0003-4103-6045</orcidid><orcidid>https://orcid.org/0000-0001-5726-4734</orcidid><orcidid>https://orcid.org/0000-0001-7904-4556</orcidid><orcidid>https://orcid.org/0000-0002-8338-9546</orcidid></search><sort><creationdate>20211201</creationdate><title>Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?</title><author>Irez, Tulay ; Ercan, Sinem ; Ciraci, Enver ; Aksoyer, Saadet Busra ; Toprak, M. Sait ; Dayioglu, Nurten ; Guralp, Onur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1076-86ae30b6c5ab77cdee6dca7048d371deb2ce79d27f712bfaa2007b9ac27438e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Apoptosis</topic><topic>Comparative analysis</topic><topic>Embryonic development</topic><topic>Glycoproteins</topic><topic>Luteinizing hormone</topic><topic>Pituitary hormones</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Irez, Tulay</creatorcontrib><creatorcontrib>Ercan, Sinem</creatorcontrib><creatorcontrib>Ciraci, Enver</creatorcontrib><creatorcontrib>Aksoyer, Saadet Busra</creatorcontrib><creatorcontrib>Toprak, M. Sait</creatorcontrib><creatorcontrib>Dayioglu, Nurten</creatorcontrib><creatorcontrib>Guralp, Onur</creatorcontrib><collection>CrossRef</collection><jtitle>Gynecology, obstetrics & reproductive medicine : GORM</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Irez, Tulay</au><au>Ercan, Sinem</au><au>Ciraci, Enver</au><au>Aksoyer, Saadet Busra</au><au>Toprak, M. Sait</au><au>Dayioglu, Nurten</au><au>Guralp, Onur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation?</atitle><jtitle>Gynecology, obstetrics & reproductive medicine : GORM</jtitle><date>2021-12-01</date><risdate>2021</risdate><volume>27</volume><issue>3</issue><spage>234</spage><epage>7</epage><pages>234-7</pages><issn>1300-4751</issn><eissn>2602-4918</eissn><abstract>OBJECTIVE: In this study, we aimed to investigate the role of the cumulus cell’s apoptosis parameter in the maturation of immature rescue oocytes. STUDY DESIGN: In this experimental study, donated immature germinal vesicle oocytes were cultured for, in vitro maturation, embryo development in matured germinal vesicle oocytes were compared with apoptotic properties of cumulus cells. RESULTS: In all of the immature oocytes after oocyte in vitro maturation, the maturation rate has been observed as 56.1% and 2PN rate as 63.0%. Afterin vitro maturation of germinal vesicle oocytes, there was no difference in apoptosis rates of the cumulus cells between mature and immature oocytes (p> 0.05). The ratio of 2PN in matured germinal vesicle oocytes showing embryo development was 35.4%. A positive correlation was found between luteinizing hormone values on day 3 and E2 values during HCG days during oocyte maturation and embryo development (p=0.021, p=0.020). In addition, it has been observed that the germinal vesicle oocytes, which have completed their maturation and developed into embryos, have high E2 values during HCG days (p=0.020).CONCLUSION: In our study, it has been demonstrated that in vitro maturation in rescue oocytes from stimulated cycles, embryo development potential could not be explained by the apoptosis parameter.</abstract><pub>Medical Network</pub><doi>10.21613/GORM.2020.1087</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3517-3046</orcidid><orcidid>https://orcid.org/0000-0001-8272-4931</orcidid><orcidid>https://orcid.org/0000-0002-5102-6599</orcidid><orcidid>https://orcid.org/0000-0003-4103-6045</orcidid><orcidid>https://orcid.org/0000-0001-5726-4734</orcidid><orcidid>https://orcid.org/0000-0001-7904-4556</orcidid><orcidid>https://orcid.org/0000-0002-8338-9546</orcidid></addata></record> |
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| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Apoptosis Comparative analysis Embryonic development Glycoproteins Luteinizing hormone Pituitary hormones |
| title | Does the Apoptosis Value of Cumulus Cells Play a Role in Rescue Oocyte in Vitro Maturation? |
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