Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia
Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. Neuron-glial antigen 2 (NG2) is not expressed by normal hematopoietic stem cells but e...
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Veröffentlicht in: | The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology 2021-04, Vol.46 (2), p.75-82 |
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container_title | The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology |
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creator | Gawaly, Amr Elkholy, Rasha Hagag, Rasha Abd El-Lateef, Amal Allam, Alzahraa |
description | Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. Neuron-glial antigen 2 (NG2) is not expressed by normal hematopoietic stem cells but expressed on blast cells in adult AML. NG2 has been incorporated in diagnostic panels for immunophenotyping of leukemic patients because of its positive predictive value for Mixed Lineage Leukemia (MLL) rearrangements.
Aims To assess NG2 expression in adult patients with AML and its correlation with disease-free survival.
Patients and methods A total of 60 patients were divided into two groups: 40 newly diagnosed patients with AML and 20 patients diagnosed as having hypersplenism used as a control group. Leukemic patients were diagnosed on the basis of clinical presentation, morphological and cytochemical examination of peripheral blood and bone marrow smears, as well as immunophenotyping criteria for diagnosis of AML. NG2 expression was evaluated in the two groups using flow cytometry.
Results No significant differences were found in both age and sex in different patient groups. NG2 expression in the patient group versus control group showed a statistically significant difference. There was no statistically significant difference regarding complete blood count, lactate dehydrogenase, and erythrocyte sedimentation rate, as well as blast percentage in the peripheral blood and in the bone marrow on comparing NG2-positive group and NG2-negative group. There is a significant increase in disease-free survival and overall survival in the negative NG2 expression than in the positive NG2 expression group (P=0.043).
Conclusion NG2 expression has a major role in the outcome of patients with AML. NG2 expression analysis can be used as a prognostic marker in newly diagnosed patients with AML. NG2 could be a target for therapy by using anti-NG2 antibody in a subset of patients with AML who do not respond to conventional therapy. |
doi_str_mv | 10.4103/ejh.ejh_61_20 |
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Aims To assess NG2 expression in adult patients with AML and its correlation with disease-free survival.
Patients and methods A total of 60 patients were divided into two groups: 40 newly diagnosed patients with AML and 20 patients diagnosed as having hypersplenism used as a control group. Leukemic patients were diagnosed on the basis of clinical presentation, morphological and cytochemical examination of peripheral blood and bone marrow smears, as well as immunophenotyping criteria for diagnosis of AML. NG2 expression was evaluated in the two groups using flow cytometry.
Results No significant differences were found in both age and sex in different patient groups. NG2 expression in the patient group versus control group showed a statistically significant difference. There was no statistically significant difference regarding complete blood count, lactate dehydrogenase, and erythrocyte sedimentation rate, as well as blast percentage in the peripheral blood and in the bone marrow on comparing NG2-positive group and NG2-negative group. There is a significant increase in disease-free survival and overall survival in the negative NG2 expression than in the positive NG2 expression group (P=0.043).
Conclusion NG2 expression has a major role in the outcome of patients with AML. NG2 expression analysis can be used as a prognostic marker in newly diagnosed patients with AML. NG2 could be a target for therapy by using anti-NG2 antibody in a subset of patients with AML who do not respond to conventional therapy.</description><identifier>ISSN: 1110-1067</identifier><identifier>EISSN: 2090-9268</identifier><identifier>DOI: 10.4103/ejh.ejh_61_20</identifier><language>eng</language><publisher>Wolters Kluwer India Pvt. Ltd</publisher><subject>Antigens ; Hematopoietic stem cells ; Medical equipment and supplies industry ; Medical research ; Medical test kit industry ; Medicine, Experimental ; Neurons</subject><ispartof>The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology, 2021-04, Vol.46 (2), p.75-82</ispartof><rights>COPYRIGHT 2021 Medknow Publications and Media Pvt. Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c276t-7126ea1054d0662f6b61ca898aebb68a5a8c5154e60b5d5d2a63b98bb497784a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,27465,27931,27932</link.rule.ids></links><search><creatorcontrib>Gawaly, Amr</creatorcontrib><creatorcontrib>Elkholy, Rasha</creatorcontrib><creatorcontrib>Hagag, Rasha</creatorcontrib><creatorcontrib>Abd El-Lateef, Amal</creatorcontrib><creatorcontrib>Allam, Alzahraa</creatorcontrib><title>Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia</title><title>The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology</title><description>Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. Neuron-glial antigen 2 (NG2) is not expressed by normal hematopoietic stem cells but expressed on blast cells in adult AML. NG2 has been incorporated in diagnostic panels for immunophenotyping of leukemic patients because of its positive predictive value for Mixed Lineage Leukemia (MLL) rearrangements.
Aims To assess NG2 expression in adult patients with AML and its correlation with disease-free survival.
Patients and methods A total of 60 patients were divided into two groups: 40 newly diagnosed patients with AML and 20 patients diagnosed as having hypersplenism used as a control group. Leukemic patients were diagnosed on the basis of clinical presentation, morphological and cytochemical examination of peripheral blood and bone marrow smears, as well as immunophenotyping criteria for diagnosis of AML. NG2 expression was evaluated in the two groups using flow cytometry.
Results No significant differences were found in both age and sex in different patient groups. NG2 expression in the patient group versus control group showed a statistically significant difference. There was no statistically significant difference regarding complete blood count, lactate dehydrogenase, and erythrocyte sedimentation rate, as well as blast percentage in the peripheral blood and in the bone marrow on comparing NG2-positive group and NG2-negative group. There is a significant increase in disease-free survival and overall survival in the negative NG2 expression than in the positive NG2 expression group (P=0.043).
Conclusion NG2 expression has a major role in the outcome of patients with AML. NG2 expression analysis can be used as a prognostic marker in newly diagnosed patients with AML. NG2 could be a target for therapy by using anti-NG2 antibody in a subset of patients with AML who do not respond to conventional therapy.</description><subject>Antigens</subject><subject>Hematopoietic stem cells</subject><subject>Medical equipment and supplies industry</subject><subject>Medical research</subject><subject>Medical test kit industry</subject><subject>Medicine, Experimental</subject><subject>Neurons</subject><issn>1110-1067</issn><issn>2090-9268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNptkdFrFDEQxoMoeNQ--h4QfNszyW6y2RehVKtCwRd9DrPJ7F162eRIsp79791yViyUYRhm-H3DDB8hbznbdpy1H_Buv13TKG4Ee0E2gg2sGYTSL8mGc84azlT_mlyWcscY40IOnMsNcZ887GIq1VsK0dFjTo_tLwgL0jTRiEtOsdkFD2GFqt9hpILi72PGUnyK1EcKbgmVgl0q0vkeQ_KOBlwOOHt4Q15NEApe_q0X5OfN5x_XX5vb71--XV_dNlb0qjY9FwqBM9k5ppSY1Ki4BT1owHFUGiRoK7nsULFROukEqHYc9Dh2Q9_rDtoL8u68dwcBjY9Tqhns7Is1V0ozLTvRtSu1fYZaw6232hRx8uv8ieD9f4I9Qqj7ksJS19fLU7A5gzanUjJO5pj9DPnecGYeXDIPDv1zaeU_nvlTChVzOYTlhNnM6A4xnZ4XmV6aR8_aPwxCm8A</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Gawaly, Amr</creator><creator>Elkholy, Rasha</creator><creator>Hagag, Rasha</creator><creator>Abd El-Lateef, Amal</creator><creator>Allam, Alzahraa</creator><general>Wolters Kluwer India Pvt. Ltd</general><general>Medknow Publications and Media Pvt. Ltd</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20210401</creationdate><title>Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia</title><author>Gawaly, Amr ; Elkholy, Rasha ; Hagag, Rasha ; Abd El-Lateef, Amal ; Allam, Alzahraa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c276t-7126ea1054d0662f6b61ca898aebb68a5a8c5154e60b5d5d2a63b98bb497784a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antigens</topic><topic>Hematopoietic stem cells</topic><topic>Medical equipment and supplies industry</topic><topic>Medical research</topic><topic>Medical test kit industry</topic><topic>Medicine, Experimental</topic><topic>Neurons</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gawaly, Amr</creatorcontrib><creatorcontrib>Elkholy, Rasha</creatorcontrib><creatorcontrib>Hagag, Rasha</creatorcontrib><creatorcontrib>Abd El-Lateef, Amal</creatorcontrib><creatorcontrib>Allam, Alzahraa</creatorcontrib><collection>CrossRef</collection><jtitle>The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gawaly, Amr</au><au>Elkholy, Rasha</au><au>Hagag, Rasha</au><au>Abd El-Lateef, Amal</au><au>Allam, Alzahraa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia</atitle><jtitle>The Egyptian journal of haematology : the official journal of the Egyptian Society of Haematology</jtitle><date>2021-04-01</date><risdate>2021</risdate><volume>46</volume><issue>2</issue><spage>75</spage><epage>82</epage><pages>75-82</pages><issn>1110-1067</issn><eissn>2090-9268</eissn><abstract>Background Acute myeloid leukemia (AML) is a hematopoietic stem cell disorder characterized by a block in differentiation of hematopoiesis, resulting in growth of a clonal population of neoplastic cells or blasts. Neuron-glial antigen 2 (NG2) is not expressed by normal hematopoietic stem cells but expressed on blast cells in adult AML. NG2 has been incorporated in diagnostic panels for immunophenotyping of leukemic patients because of its positive predictive value for Mixed Lineage Leukemia (MLL) rearrangements.
Aims To assess NG2 expression in adult patients with AML and its correlation with disease-free survival.
Patients and methods A total of 60 patients were divided into two groups: 40 newly diagnosed patients with AML and 20 patients diagnosed as having hypersplenism used as a control group. Leukemic patients were diagnosed on the basis of clinical presentation, morphological and cytochemical examination of peripheral blood and bone marrow smears, as well as immunophenotyping criteria for diagnosis of AML. NG2 expression was evaluated in the two groups using flow cytometry.
Results No significant differences were found in both age and sex in different patient groups. NG2 expression in the patient group versus control group showed a statistically significant difference. There was no statistically significant difference regarding complete blood count, lactate dehydrogenase, and erythrocyte sedimentation rate, as well as blast percentage in the peripheral blood and in the bone marrow on comparing NG2-positive group and NG2-negative group. There is a significant increase in disease-free survival and overall survival in the negative NG2 expression than in the positive NG2 expression group (P=0.043).
Conclusion NG2 expression has a major role in the outcome of patients with AML. NG2 expression analysis can be used as a prognostic marker in newly diagnosed patients with AML. NG2 could be a target for therapy by using anti-NG2 antibody in a subset of patients with AML who do not respond to conventional therapy.</abstract><pub>Wolters Kluwer India Pvt. Ltd</pub><doi>10.4103/ejh.ejh_61_20</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Antigens Hematopoietic stem cells Medical equipment and supplies industry Medical research Medical test kit industry Medicine, Experimental Neurons |
title | Diagnostic and prognostic value of neuron-glial antigen 2 expression in adult acute myeloid leukemia |
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