Simultaneous quantification of the major flavonoids from wild spinach by UHPLC-HRMS and their neuroprotective effects in a model of H[sub.2]O[sub.2]-induced oxidative stress on SH-SY5Y cells
A modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach (Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin...
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Veröffentlicht in: | Farmacija 2021-09 (3), p.657 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A modified UHPLC-HRMS method for simultaneous quantification of eight flavonoids from the aerial parts of the wild spinach (Chenopodium bonus-henricus L.) was re-validated for specificity, the limit of detection and quantitation limit, linearity, accuracy, and precision. The glycosides of spinacetin (Chbhnf-04, Chbhnf-06, and Chbhnf-08) and patuletin (Chbhnf-01) were the predominant compounds. The total amount of assayed flavonoids from the aerial parts of a title plant was estimated to be 1.82% and 1.4% in two different populations from Vitosha Mountain (Bulgaria). The neuroprotective properties of compounds Chbhnf-02, Chbhnf-04, Chbhnf-06, Chbhnf-07, Chbhnf-08 were further assessed using a model of H[sub.2]O[sub.2]-induced oxidative stress on human neuroblastoma SH-SY5Y cells. All tested flavonoids demonstrated statistically significant neuroprotective activity close to that of silibinin. Patuletin (Chbhnf-07) and spinacetin (Chbhnf-08) triglycosides showed the most protective effects at the lowest concentration of 50 µM. Keywords: Chenopodium bonus-henricus , flavonoids, neuroprotection, oxidative stress, quantification |
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ISSN: | 0428-0296 |
DOI: | 10.3897/pharmacia.68.e71030 |