MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase [alpha]
Choline kinase (ChK) catalyzes the first step in the CDP-choline pathway for the synthesis of phosphatidylcholine. The a isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attentio...
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Veröffentlicht in: | Oncology letters 2021-03, Vol.21 (3), p.1 |
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description | Choline kinase (ChK) catalyzes the first step in the CDP-choline pathway for the synthesis of phosphatidylcholine. The a isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attention being paid to ChK expression, as well as its activity and inhibition in cancer, there are only limited studies available on the regulation of ChK, including its regulation by microRNAs (miRNAs/miRs). The dysregulation of gene expression by miRNAs is a common cause for carcinogenesis. In the present study, miR-367-3p was predicted to target the 3'-untranslated region (UTR) of the ChK [alpha] (chka) mRNA transcript. The binding of miR-367-3p to the 3'-UTR of chka was validated by a luciferase assay. The effects of the miR-367-3p mimic on chka gene and protein expression levels were determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. miR-367-3p significantly downregulated the expression of chka to ~60% of the negative control. Cells transfected with miR-367-3p exhibited higher levels of apoptosis and a lower cell migration compared with the control. To the best of our knowledge, the present study provided the first experimental evidence of the regulation of chka expression by miR-367-3p. The pro-apoptotic and suppressive effects of miR-367-3p on cell migration were similar to the anticancer effects resulting from the inhibition of ChK enzyme activity or the knockdown of chka gene expression by small interfering RNA. Therefore, these findings may potentially lead to the use of miR-367-3p in anticancer strategies that target ChK. Key words: choline kinase, microRNA, gene expression, cancer, apoptosis |
doi_str_mv | 10.3892/ol.2021.12444 |
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The a isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attention being paid to ChK expression, as well as its activity and inhibition in cancer, there are only limited studies available on the regulation of ChK, including its regulation by microRNAs (miRNAs/miRs). The dysregulation of gene expression by miRNAs is a common cause for carcinogenesis. In the present study, miR-367-3p was predicted to target the 3'-untranslated region (UTR) of the ChK [alpha] (chka) mRNA transcript. The binding of miR-367-3p to the 3'-UTR of chka was validated by a luciferase assay. The effects of the miR-367-3p mimic on chka gene and protein expression levels were determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. miR-367-3p significantly downregulated the expression of chka to ~60% of the negative control. Cells transfected with miR-367-3p exhibited higher levels of apoptosis and a lower cell migration compared with the control. To the best of our knowledge, the present study provided the first experimental evidence of the regulation of chka expression by miR-367-3p. The pro-apoptotic and suppressive effects of miR-367-3p on cell migration were similar to the anticancer effects resulting from the inhibition of ChK enzyme activity or the knockdown of chka gene expression by small interfering RNA. Therefore, these findings may potentially lead to the use of miR-367-3p in anticancer strategies that target ChK. Key words: choline kinase, microRNA, gene expression, cancer, apoptosis</description><identifier>ISSN: 1792-1074</identifier><identifier>DOI: 10.3892/ol.2021.12444</identifier><language>eng</language><publisher>Spandidos Publications</publisher><subject>Analysis ; Apoptosis ; Cancer ; Care and treatment ; Choline ; Cytidine diphosphate choline ; Enzymes ; Genes ; Genetic research ; Genetic transcription ; MicroRNA ; Pharmaceutical industry ; Prevention ; Scientific equipment and supplies industry</subject><ispartof>Oncology letters, 2021-03, Vol.21 (3), p.1</ispartof><rights>COPYRIGHT 2021 Spandidos Publications</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Raikundalia, Sweta</creatorcontrib><creatorcontrib>Sa'dom, Siti Aisyah Faten Mohamed</creatorcontrib><creatorcontrib>Few, Ling Ling</creatorcontrib><creatorcontrib>Too, Wei Cun See</creatorcontrib><title>MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase [alpha]</title><title>Oncology letters</title><description>Choline kinase (ChK) catalyzes the first step in the CDP-choline pathway for the synthesis of phosphatidylcholine. The a isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attention being paid to ChK expression, as well as its activity and inhibition in cancer, there are only limited studies available on the regulation of ChK, including its regulation by microRNAs (miRNAs/miRs). The dysregulation of gene expression by miRNAs is a common cause for carcinogenesis. In the present study, miR-367-3p was predicted to target the 3'-untranslated region (UTR) of the ChK [alpha] (chka) mRNA transcript. The binding of miR-367-3p to the 3'-UTR of chka was validated by a luciferase assay. The effects of the miR-367-3p mimic on chka gene and protein expression levels were determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. miR-367-3p significantly downregulated the expression of chka to ~60% of the negative control. Cells transfected with miR-367-3p exhibited higher levels of apoptosis and a lower cell migration compared with the control. To the best of our knowledge, the present study provided the first experimental evidence of the regulation of chka expression by miR-367-3p. The pro-apoptotic and suppressive effects of miR-367-3p on cell migration were similar to the anticancer effects resulting from the inhibition of ChK enzyme activity or the knockdown of chka gene expression by small interfering RNA. Therefore, these findings may potentially lead to the use of miR-367-3p in anticancer strategies that target ChK. Key words: choline kinase, microRNA, gene expression, cancer, apoptosis</description><subject>Analysis</subject><subject>Apoptosis</subject><subject>Cancer</subject><subject>Care and treatment</subject><subject>Choline</subject><subject>Cytidine diphosphate choline</subject><subject>Enzymes</subject><subject>Genes</subject><subject>Genetic research</subject><subject>Genetic transcription</subject><subject>MicroRNA</subject><subject>Pharmaceutical industry</subject><subject>Prevention</subject><subject>Scientific equipment and supplies industry</subject><issn>1792-1074</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptkE1LxDAQQHtQcFn36D0geGtt0q_kuCyuCrsKoieRZZpO2mialKZF_Qv-aqvuQcGZwwwz781hguCExlHCBTt3JmIxoxFlaZoeBDNaCBbSuEiPgoX3z_EUWU45z2fBx1bL3t3dLMMkL8KkI9pWo0RPoHPd4LyeOlsRP3Zdj95Pi1bXPQzaWeIU2a7WYUEkGuNJ-U4q92p7rEczAbYmQ4ME377FPd-MLVgiG2e0RfKiLXgkj2C6Bp6Og0MFxuNiX-fBw_rifnUVbm4vr1fLTVjTnA8hiFLEkKlcFEqUSlZlKhnkVCoGDDOoAEXCOM0zXnBUMU8qDklWsBJVxkSZzIPTn7s1GNxpq9zQg2y1l7tlniUx44yJiYr-oaassNXSWVR6mv8Rzn4JDYIZGu_M-PUq_xv8BDTygUU</recordid><startdate>20210301</startdate><enddate>20210301</enddate><creator>Raikundalia, Sweta</creator><creator>Sa'dom, Siti Aisyah Faten Mohamed</creator><creator>Few, Ling Ling</creator><creator>Too, Wei Cun See</creator><general>Spandidos Publications</general><scope/></search><sort><creationdate>20210301</creationdate><title>MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase [alpha]</title><author>Raikundalia, Sweta ; Sa'dom, Siti Aisyah Faten Mohamed ; Few, Ling Ling ; Too, Wei Cun See</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g168t-a9b90a5f697f9bfcdb4c2a61cf2a2e5adae9328165878ef083d8a3572bef529b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analysis</topic><topic>Apoptosis</topic><topic>Cancer</topic><topic>Care and treatment</topic><topic>Choline</topic><topic>Cytidine diphosphate choline</topic><topic>Enzymes</topic><topic>Genes</topic><topic>Genetic research</topic><topic>Genetic transcription</topic><topic>MicroRNA</topic><topic>Pharmaceutical industry</topic><topic>Prevention</topic><topic>Scientific equipment and supplies industry</topic><toplevel>online_resources</toplevel><creatorcontrib>Raikundalia, Sweta</creatorcontrib><creatorcontrib>Sa'dom, Siti Aisyah Faten Mohamed</creatorcontrib><creatorcontrib>Few, Ling Ling</creatorcontrib><creatorcontrib>Too, Wei Cun See</creatorcontrib><jtitle>Oncology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raikundalia, Sweta</au><au>Sa'dom, Siti Aisyah Faten Mohamed</au><au>Few, Ling Ling</au><au>Too, Wei Cun See</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase [alpha]</atitle><jtitle>Oncology letters</jtitle><date>2021-03-01</date><risdate>2021</risdate><volume>21</volume><issue>3</issue><spage>1</spage><pages>1-</pages><issn>1792-1074</issn><abstract>Choline kinase (ChK) catalyzes the first step in the CDP-choline pathway for the synthesis of phosphatidylcholine. The a isoform of this enzyme is overexpressed in various types of cancer and its inhibition or downregulation has been applied as an anticancer strategy. In spite of increasing attention being paid to ChK expression, as well as its activity and inhibition in cancer, there are only limited studies available on the regulation of ChK, including its regulation by microRNAs (miRNAs/miRs). The dysregulation of gene expression by miRNAs is a common cause for carcinogenesis. In the present study, miR-367-3p was predicted to target the 3'-untranslated region (UTR) of the ChK [alpha] (chka) mRNA transcript. The binding of miR-367-3p to the 3'-UTR of chka was validated by a luciferase assay. The effects of the miR-367-3p mimic on chka gene and protein expression levels were determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis, respectively. miR-367-3p significantly downregulated the expression of chka to ~60% of the negative control. Cells transfected with miR-367-3p exhibited higher levels of apoptosis and a lower cell migration compared with the control. To the best of our knowledge, the present study provided the first experimental evidence of the regulation of chka expression by miR-367-3p. The pro-apoptotic and suppressive effects of miR-367-3p on cell migration were similar to the anticancer effects resulting from the inhibition of ChK enzyme activity or the knockdown of chka gene expression by small interfering RNA. Therefore, these findings may potentially lead to the use of miR-367-3p in anticancer strategies that target ChK. Key words: choline kinase, microRNA, gene expression, cancer, apoptosis</abstract><pub>Spandidos Publications</pub><doi>10.3892/ol.2021.12444</doi></addata></record> |
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source | Spandidos Publications Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
subjects | Analysis Apoptosis Cancer Care and treatment Choline Cytidine diphosphate choline Enzymes Genes Genetic research Genetic transcription MicroRNA Pharmaceutical industry Prevention Scientific equipment and supplies industry |
title | MicroRNA-367-3p induces apoptosis and suppresses migration of MCF-7 cells by downregulating the expression of human choline kinase [alpha] |
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