Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme
Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose...
Gespeichert in:
| Veröffentlicht in: | Analytical and bioanalytical chemistry 2021-02, Vol.413 (5), p.1279-1291 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1291 |
|---|---|
| container_issue | 5 |
| container_start_page | 1279 |
| container_title | Analytical and bioanalytical chemistry |
| container_volume | 413 |
| creator | Naveen Prasad, Sanjana Weerathunge, Pabudi Karim, Md. Nurul Anderson, Samuel Hashmi, Sabeen Mariathomas, Pyria D. Bansal, Vipul Ramanathan, Rajesh |
| description | Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose oxidase (GOx)/horseradish peroxidase (HRP) assay remains the gold standard for pathological detection of glucose. A key restriction is poor stability of HRP and its suicidal inactivation by hydrogen peroxide, a key intermediate of the GOx-driven reaction. An alternative is to replace HRP with a robust inorganic enzyme-mimic or NanoZyme. While colloidal NanoZymes show promise in glucose sensing, they detect low concentrations of glucose, while urine has high (mM) glucose concentration. In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5–15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at a single pH without the need to use different pH conditions as used during the gold standard assay. These outcomes outline the high robustness of the NanoZyme sensing system for direct detection of glucose in human urine.
Graphical abstract |
| doi_str_mv | 10.1007/s00216-020-03090-w |
| format | Article |
| fullrecord | <record><control><sourceid>gale_webof</sourceid><recordid>TN_cdi_gale_infotracmisc_A652303329</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A652303329</galeid><sourcerecordid>A652303329</sourcerecordid><originalsourceid>FETCH-LOGICAL-c479t-249a68a4fb53fe9b36e19e40421ac53d559eb9bec8e1b8faf39cab38590a361d3</originalsourceid><addsrcrecordid>eNqNkl9rFDEUxYMotq5-AR9kwBdBpt5MJtnksSz1D5T6og_6EjKZmzVlJlmTmS799madukURkTzkcvmdwyEnhDyncEYB1m8yQENFDQ3UwEBBvX9ATqmgsm4Eh4fHuW1OyJOcrwEol1Q8JieMMaWkhFPy5SqG2ocbk_0NVj1OaCcfQxVdtR1mGzNWPlTf5tGEak4-YDVnH7aVqWwcYqq3GDCZ6bCycbfDVF2ZEL_ejviUPHJmyPjs7l6Rz28vPm3e15cf333YnF_Wtl2rqW5aZYQ0res4c6g6JpAqbKFtqLGc9Zwr7FSHViLtpDOOKWs6JrkCwwTt2Yq8Wnx3KX6fMU969NniMJiAcc66adecKVn8CvryD_Q6zimUdIVSIAVfA7-ntmZA7YOLUzL2YKrPBW8YMNaoQp39hSqnx9HbGND5sv9N0CwCm2LOCZ3eJT-adKsp6EOfeulTlz71zz71vohe3CWeuxH7o-RXgQV4vQB77KLL1mOweMQAQACnrWBlKjFWRP4_vfGTOfyFTZzDVKRskeaChy2m-7f7R_4fq5TKng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2490865705</pqid></control><display><type>article</type><title>Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme</title><source>SpringerNature Journals</source><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Naveen Prasad, Sanjana ; Weerathunge, Pabudi ; Karim, Md. Nurul ; Anderson, Samuel ; Hashmi, Sabeen ; Mariathomas, Pyria D. ; Bansal, Vipul ; Ramanathan, Rajesh</creator><creatorcontrib>Naveen Prasad, Sanjana ; Weerathunge, Pabudi ; Karim, Md. Nurul ; Anderson, Samuel ; Hashmi, Sabeen ; Mariathomas, Pyria D. ; Bansal, Vipul ; Ramanathan, Rajesh</creatorcontrib><description>Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose oxidase (GOx)/horseradish peroxidase (HRP) assay remains the gold standard for pathological detection of glucose. A key restriction is poor stability of HRP and its suicidal inactivation by hydrogen peroxide, a key intermediate of the GOx-driven reaction. An alternative is to replace HRP with a robust inorganic enzyme-mimic or NanoZyme. While colloidal NanoZymes show promise in glucose sensing, they detect low concentrations of glucose, while urine has high (mM) glucose concentration. In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5–15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at a single pH without the need to use different pH conditions as used during the gold standard assay. These outcomes outline the high robustness of the NanoZyme sensing system for direct detection of glucose in human urine.
Graphical abstract</description><identifier>ISSN: 1618-2642</identifier><identifier>EISSN: 1618-2650</identifier><identifier>DOI: 10.1007/s00216-020-03090-w</identifier><identifier>PMID: 33399880</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Analysis ; Analytical Chemistry ; Biochemical Research Methods ; Biochemistry ; Biochemistry & Molecular Biology ; Characterization and Evaluation of Materials ; Charge transfer ; Chemical properties ; Chemistry ; Chemistry and Materials Science ; Chemistry, Analytical ; Chemoreception ; Colorimetry ; Copper ; Copper compounds ; Dextrose ; Diabetes mellitus ; Diagnosis, Noninvasive ; Dilution ; Food Science ; Glucose ; Glucose oxidase ; Horseradish peroxidase ; Human wastes ; Hydrogen peroxide ; Inactivation ; Laboratory Medicine ; Life Sciences & Biomedicine ; Low concentrations ; Mathematical analysis ; Matrix methods ; Methods ; Monitoring/Environmental Analysis ; Nanotechnology ; Oxidation ; Peroxidase ; pH effects ; Physical Sciences ; Research Paper ; Science & Technology ; Substrates ; Urine</subject><ispartof>Analytical and bioanalytical chemistry, 2021-02, Vol.413 (5), p.1279-1291</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>50</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000605146300003</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c479t-249a68a4fb53fe9b36e19e40421ac53d559eb9bec8e1b8faf39cab38590a361d3</citedby><cites>FETCH-LOGICAL-c479t-249a68a4fb53fe9b36e19e40421ac53d559eb9bec8e1b8faf39cab38590a361d3</cites><orcidid>0000-0002-9331-9269 ; 0000-0002-3354-4317</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00216-020-03090-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00216-020-03090-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,39263,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33399880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naveen Prasad, Sanjana</creatorcontrib><creatorcontrib>Weerathunge, Pabudi</creatorcontrib><creatorcontrib>Karim, Md. Nurul</creatorcontrib><creatorcontrib>Anderson, Samuel</creatorcontrib><creatorcontrib>Hashmi, Sabeen</creatorcontrib><creatorcontrib>Mariathomas, Pyria D.</creatorcontrib><creatorcontrib>Bansal, Vipul</creatorcontrib><creatorcontrib>Ramanathan, Rajesh</creatorcontrib><title>Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme</title><title>Analytical and bioanalytical chemistry</title><addtitle>Anal Bioanal Chem</addtitle><addtitle>ANAL BIOANAL CHEM</addtitle><addtitle>Anal Bioanal Chem</addtitle><description>Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose oxidase (GOx)/horseradish peroxidase (HRP) assay remains the gold standard for pathological detection of glucose. A key restriction is poor stability of HRP and its suicidal inactivation by hydrogen peroxide, a key intermediate of the GOx-driven reaction. An alternative is to replace HRP with a robust inorganic enzyme-mimic or NanoZyme. While colloidal NanoZymes show promise in glucose sensing, they detect low concentrations of glucose, while urine has high (mM) glucose concentration. In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5–15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at a single pH without the need to use different pH conditions as used during the gold standard assay. These outcomes outline the high robustness of the NanoZyme sensing system for direct detection of glucose in human urine.
Graphical abstract</description><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Biochemical Research Methods</subject><subject>Biochemistry</subject><subject>Biochemistry & Molecular Biology</subject><subject>Characterization and Evaluation of Materials</subject><subject>Charge transfer</subject><subject>Chemical properties</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry, Analytical</subject><subject>Chemoreception</subject><subject>Colorimetry</subject><subject>Copper</subject><subject>Copper compounds</subject><subject>Dextrose</subject><subject>Diabetes mellitus</subject><subject>Diagnosis, Noninvasive</subject><subject>Dilution</subject><subject>Food Science</subject><subject>Glucose</subject><subject>Glucose oxidase</subject><subject>Horseradish peroxidase</subject><subject>Human wastes</subject><subject>Hydrogen peroxide</subject><subject>Inactivation</subject><subject>Laboratory Medicine</subject><subject>Life Sciences & Biomedicine</subject><subject>Low concentrations</subject><subject>Mathematical analysis</subject><subject>Matrix methods</subject><subject>Methods</subject><subject>Monitoring/Environmental Analysis</subject><subject>Nanotechnology</subject><subject>Oxidation</subject><subject>Peroxidase</subject><subject>pH effects</subject><subject>Physical Sciences</subject><subject>Research Paper</subject><subject>Science & Technology</subject><subject>Substrates</subject><subject>Urine</subject><issn>1618-2642</issn><issn>1618-2650</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkl9rFDEUxYMotq5-AR9kwBdBpt5MJtnksSz1D5T6og_6EjKZmzVlJlmTmS799madukURkTzkcvmdwyEnhDyncEYB1m8yQENFDQ3UwEBBvX9ATqmgsm4Eh4fHuW1OyJOcrwEol1Q8JieMMaWkhFPy5SqG2ocbk_0NVj1OaCcfQxVdtR1mGzNWPlTf5tGEak4-YDVnH7aVqWwcYqq3GDCZ6bCycbfDVF2ZEL_ejviUPHJmyPjs7l6Rz28vPm3e15cf333YnF_Wtl2rqW5aZYQ0res4c6g6JpAqbKFtqLGc9Zwr7FSHViLtpDOOKWs6JrkCwwTt2Yq8Wnx3KX6fMU969NniMJiAcc66adecKVn8CvryD_Q6zimUdIVSIAVfA7-ntmZA7YOLUzL2YKrPBW8YMNaoQp39hSqnx9HbGND5sv9N0CwCm2LOCZ3eJT-adKsp6EOfeulTlz71zz71vohe3CWeuxH7o-RXgQV4vQB77KLL1mOweMQAQACnrWBlKjFWRP4_vfGTOfyFTZzDVKRskeaChy2m-7f7R_4fq5TKng</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Naveen Prasad, Sanjana</creator><creator>Weerathunge, Pabudi</creator><creator>Karim, Md. Nurul</creator><creator>Anderson, Samuel</creator><creator>Hashmi, Sabeen</creator><creator>Mariathomas, Pyria D.</creator><creator>Bansal, Vipul</creator><creator>Ramanathan, Rajesh</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature</general><general>Springer</general><general>Springer Nature B.V</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>F28</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H8D</scope><scope>H8G</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KB.</scope><scope>KR7</scope><scope>L7M</scope><scope>LK8</scope><scope>L~C</scope><scope>L~D</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9331-9269</orcidid><orcidid>https://orcid.org/0000-0002-3354-4317</orcidid></search><sort><creationdate>20210201</creationdate><title>Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme</title><author>Naveen Prasad, Sanjana ; Weerathunge, Pabudi ; Karim, Md. Nurul ; Anderson, Samuel ; Hashmi, Sabeen ; Mariathomas, Pyria D. ; Bansal, Vipul ; Ramanathan, Rajesh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-249a68a4fb53fe9b36e19e40421ac53d559eb9bec8e1b8faf39cab38590a361d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Biochemical Research Methods</topic><topic>Biochemistry</topic><topic>Biochemistry & Molecular Biology</topic><topic>Characterization and Evaluation of Materials</topic><topic>Charge transfer</topic><topic>Chemical properties</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry, Analytical</topic><topic>Chemoreception</topic><topic>Colorimetry</topic><topic>Copper</topic><topic>Copper compounds</topic><topic>Dextrose</topic><topic>Diabetes mellitus</topic><topic>Diagnosis, Noninvasive</topic><topic>Dilution</topic><topic>Food Science</topic><topic>Glucose</topic><topic>Glucose oxidase</topic><topic>Horseradish peroxidase</topic><topic>Human wastes</topic><topic>Hydrogen peroxide</topic><topic>Inactivation</topic><topic>Laboratory Medicine</topic><topic>Life Sciences & Biomedicine</topic><topic>Low concentrations</topic><topic>Mathematical analysis</topic><topic>Matrix methods</topic><topic>Methods</topic><topic>Monitoring/Environmental Analysis</topic><topic>Nanotechnology</topic><topic>Oxidation</topic><topic>Peroxidase</topic><topic>pH effects</topic><topic>Physical Sciences</topic><topic>Research Paper</topic><topic>Science & Technology</topic><topic>Substrates</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naveen Prasad, Sanjana</creatorcontrib><creatorcontrib>Weerathunge, Pabudi</creatorcontrib><creatorcontrib>Karim, Md. Nurul</creatorcontrib><creatorcontrib>Anderson, Samuel</creatorcontrib><creatorcontrib>Hashmi, Sabeen</creatorcontrib><creatorcontrib>Mariathomas, Pyria D.</creatorcontrib><creatorcontrib>Bansal, Vipul</creatorcontrib><creatorcontrib>Ramanathan, Rajesh</creatorcontrib><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Web of Science - Science Citation Index Expanded - 2021</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>SciTech Premium Collection</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical and bioanalytical chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naveen Prasad, Sanjana</au><au>Weerathunge, Pabudi</au><au>Karim, Md. Nurul</au><au>Anderson, Samuel</au><au>Hashmi, Sabeen</au><au>Mariathomas, Pyria D.</au><au>Bansal, Vipul</au><au>Ramanathan, Rajesh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme</atitle><jtitle>Analytical and bioanalytical chemistry</jtitle><stitle>Anal Bioanal Chem</stitle><stitle>ANAL BIOANAL CHEM</stitle><addtitle>Anal Bioanal Chem</addtitle><date>2021-02-01</date><risdate>2021</risdate><volume>413</volume><issue>5</issue><spage>1279</spage><epage>1291</epage><pages>1279-1291</pages><issn>1618-2642</issn><eissn>1618-2650</eissn><abstract>Renal complications are long-term effect of diabetes mellitus where glucose is excreted in urine. Therefore, reliable glucose detection in urine is critical. While commercial urine strips offer a simple way to detect urine sugar, poor sensitivity and low reliability limit their use. A hybrid glucose oxidase (GOx)/horseradish peroxidase (HRP) assay remains the gold standard for pathological detection of glucose. A key restriction is poor stability of HRP and its suicidal inactivation by hydrogen peroxide, a key intermediate of the GOx-driven reaction. An alternative is to replace HRP with a robust inorganic enzyme-mimic or NanoZyme. While colloidal NanoZymes show promise in glucose sensing, they detect low concentrations of glucose, while urine has high (mM) glucose concentration. In this study, a free-standing copper NanoZyme is used for the colorimetric detection of glucose in human urine. The sensor could operate in a biologically relevant dynamic linear range of 0.5–15 mM, while showing minimal sample matrix effect such that glucose could be detected in urine without significant sample processing or dilution. This ability could be attributed to the Cu NanoZyme that for the first time showed an ability to promote the oxidation of a TMB substrate to its double oxidation diimine product rather than the charge-transfer complex product commonly observed. Additionally, the sensor could operate at a single pH without the need to use different pH conditions as used during the gold standard assay. These outcomes outline the high robustness of the NanoZyme sensing system for direct detection of glucose in human urine.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33399880</pmid><doi>10.1007/s00216-020-03090-w</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9331-9269</orcidid><orcidid>https://orcid.org/0000-0002-3354-4317</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1618-2642 |
| ispartof | Analytical and bioanalytical chemistry, 2021-02, Vol.413 (5), p.1279-1291 |
| issn | 1618-2642 1618-2650 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A652303329 |
| source | SpringerNature Journals; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Analysis Analytical Chemistry Biochemical Research Methods Biochemistry Biochemistry & Molecular Biology Characterization and Evaluation of Materials Charge transfer Chemical properties Chemistry Chemistry and Materials Science Chemistry, Analytical Chemoreception Colorimetry Copper Copper compounds Dextrose Diabetes mellitus Diagnosis, Noninvasive Dilution Food Science Glucose Glucose oxidase Horseradish peroxidase Human wastes Hydrogen peroxide Inactivation Laboratory Medicine Life Sciences & Biomedicine Low concentrations Mathematical analysis Matrix methods Methods Monitoring/Environmental Analysis Nanotechnology Oxidation Peroxidase pH effects Physical Sciences Research Paper Science & Technology Substrates Urine |
| title | Non-invasive detection of glucose in human urine using a color-generating copper NanoZyme |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T08%3A55%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_webof&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Non-invasive%20detection%20of%20glucose%20in%20human%20urine%20using%20a%20color-generating%20copper%20NanoZyme&rft.jtitle=Analytical%20and%20bioanalytical%20chemistry&rft.au=Naveen%20Prasad,%20Sanjana&rft.date=2021-02-01&rft.volume=413&rft.issue=5&rft.spage=1279&rft.epage=1291&rft.pages=1279-1291&rft.issn=1618-2642&rft.eissn=1618-2650&rft_id=info:doi/10.1007/s00216-020-03090-w&rft_dat=%3Cgale_webof%3EA652303329%3C/gale_webof%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2490865705&rft_id=info:pmid/33399880&rft_galeid=A652303329&rfr_iscdi=true |