HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway
MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-[beta]1 signaling. The present study was designed to investigate the role of TGF-[beta]1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co-transporting polypeptide (NTCP)-transfected Hu...
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| Veröffentlicht in: | Experimental and therapeutic medicine 2021-02, Vol.21 (2) |
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| creator | Li, Wenting Yu, Xiaolan Chen, Xiliu Wang, Zheng Yin, Ming Zhao, Zonghao Zhu, Chuanwu |
| description | MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-[beta]1 signaling. The present study was designed to investigate the role of TGF-[beta]1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co-transporting polypeptide (NTCP)-transfected Huh7.5.1 cells were co-cultured with LX2 cells to simulate HBV infection in the present study. A total of 29 patients with chronic HBV infection were enrolled. Cells were transfected with miR-21-5p mimic or inhibitor with or without TGF-[beta]1 stimulation. The demographic, biochemical and virological data from the 29 patients were analyzed and liver tissues were collected. miR-21-5p levels and the mRNA and protein expression of a-smooth muscle actin (SMA), collagen type 1 [alpha] 1 (CoL1A1), tissue inhibitor of metalloproteinase (TIMP)-1 and Smad from liver cells or tissues were detected by quantitative PCR analysis and western blotting, respectively. Cell viability was observed, and the liver fibrosis score was evaluated. The association between miR-21-5p and liver fibrosis was evaluated by correlation analysis. HBV infection upregulated TGF-[beta]1/miR-21-5p mRNA expression in NTCP-Huh7.5.1 cells compared with mock infection (P |
| doi_str_mv | 10.3892/etm.2020.9600 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A649552312</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A649552312</galeid><sourcerecordid>A649552312</sourcerecordid><originalsourceid>FETCH-LOGICAL-g672-b7db0d028f3042c27731559dfac3c244039434462dcc4abecb475632852f497f3</originalsourceid><addsrcrecordid>eNptjMFLwzAYxXNQcMwdvQc8p0u-JE1ycw63CQNBiheRkaTJFlnb0dSJ_70FPXjwvcODx-89hG4YLbg2MA9DUwAFWpiS0gs0YcoAoUazKzTL-Z2OkiXTWk7Q3eb-Bae2_vAh42M6hx7H5Poup4zPyeLhEHC1XpFXFwb7xuZNeibAiDzhkx0On_brGl1Ge8xh9ptTVK0equWGbJ_Wj8vFluxLBcSp2tGago6cCvCgFGdSmjpazz0IQbkRXIgSau-FdcE7oWTJQUuIwqjIp-j253Zvj2GX2tgNvfVNyn63KIWREjiDkSr-oUbXoUm-a0NMY_9n8A2mkFZF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway</title><source>PubMed Central</source><creator>Li, Wenting ; Yu, Xiaolan ; Chen, Xiliu ; Wang, Zheng ; Yin, Ming ; Zhao, Zonghao ; Zhu, Chuanwu</creator><creatorcontrib>Li, Wenting ; Yu, Xiaolan ; Chen, Xiliu ; Wang, Zheng ; Yin, Ming ; Zhao, Zonghao ; Zhu, Chuanwu</creatorcontrib><description>MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-[beta]1 signaling. The present study was designed to investigate the role of TGF-[beta]1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co-transporting polypeptide (NTCP)-transfected Huh7.5.1 cells were co-cultured with LX2 cells to simulate HBV infection in the present study. A total of 29 patients with chronic HBV infection were enrolled. Cells were transfected with miR-21-5p mimic or inhibitor with or without TGF-[beta]1 stimulation. The demographic, biochemical and virological data from the 29 patients were analyzed and liver tissues were collected. miR-21-5p levels and the mRNA and protein expression of a-smooth muscle actin (SMA), collagen type 1 [alpha] 1 (CoL1A1), tissue inhibitor of metalloproteinase (TIMP)-1 and Smad from liver cells or tissues were detected by quantitative PCR analysis and western blotting, respectively. Cell viability was observed, and the liver fibrosis score was evaluated. The association between miR-21-5p and liver fibrosis was evaluated by correlation analysis. HBV infection upregulated TGF-[beta]1/miR-21-5p mRNA expression in NTCP-Huh7.5.1 cells compared with mock infection (P <0.05). TGF-[beta]1 incubation significantly increased miR-21-5p levels, as well as the mRNA and protein expression of [alpha]-SMA, CoL1A1 and TIMP-1, and reduced Smad7 expression in LX2 cells compared with the normal group, and these effects were counteracted by miR-21-5p inhibitor (P <0.05). miR-21-5p overexpression also contributed to TGF-[beta]1-induced a-SMA, CoL1A1 and TIMP-1 expression in LX2 cells (P <0.05). Co-culture with HBV-infected NTCP-Huh7.5.1 cells upregu lated TGF-[beta]1/miR-21-5p activity and CoL1A1 expression in LX2 cells compared with normal control, which were significantly reduced by miR-21-5p inhibitor (P <0.05). miR-21-5p levels were significantly correlated with the liver fibrosis score (r=0.888; P <0.05). These data demonstrated that HBV induced liver fibrosis via the TGF-[beta]1/miR-21-5p pathway and suggested that miR-21-5p may be an effective anti-fibrosis target. Key words: hepatitis B virus, liver fibrosis, microRNA-21-5p, TGF-[beta]1</description><identifier>ISSN: 1792-0981</identifier><identifier>DOI: 10.3892/etm.2020.9600</identifier><language>eng</language><publisher>Spandidos Publications</publisher><subject>Cellular signal transduction ; Complications and side effects ; Development and progression ; Fibrosis ; Genetic aspects ; Health aspects ; Hepatitis B ; Liver diseases ; MicroRNA ; Transforming growth factors</subject><ispartof>Experimental and therapeutic medicine, 2021-02, Vol.21 (2)</ispartof><rights>COPYRIGHT 2021 Spandidos Publications</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Li, Wenting</creatorcontrib><creatorcontrib>Yu, Xiaolan</creatorcontrib><creatorcontrib>Chen, Xiliu</creatorcontrib><creatorcontrib>Wang, Zheng</creatorcontrib><creatorcontrib>Yin, Ming</creatorcontrib><creatorcontrib>Zhao, Zonghao</creatorcontrib><creatorcontrib>Zhu, Chuanwu</creatorcontrib><title>HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway</title><title>Experimental and therapeutic medicine</title><description>MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-[beta]1 signaling. The present study was designed to investigate the role of TGF-[beta]1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co-transporting polypeptide (NTCP)-transfected Huh7.5.1 cells were co-cultured with LX2 cells to simulate HBV infection in the present study. A total of 29 patients with chronic HBV infection were enrolled. Cells were transfected with miR-21-5p mimic or inhibitor with or without TGF-[beta]1 stimulation. The demographic, biochemical and virological data from the 29 patients were analyzed and liver tissues were collected. miR-21-5p levels and the mRNA and protein expression of a-smooth muscle actin (SMA), collagen type 1 [alpha] 1 (CoL1A1), tissue inhibitor of metalloproteinase (TIMP)-1 and Smad from liver cells or tissues were detected by quantitative PCR analysis and western blotting, respectively. Cell viability was observed, and the liver fibrosis score was evaluated. The association between miR-21-5p and liver fibrosis was evaluated by correlation analysis. HBV infection upregulated TGF-[beta]1/miR-21-5p mRNA expression in NTCP-Huh7.5.1 cells compared with mock infection (P <0.05). TGF-[beta]1 incubation significantly increased miR-21-5p levels, as well as the mRNA and protein expression of [alpha]-SMA, CoL1A1 and TIMP-1, and reduced Smad7 expression in LX2 cells compared with the normal group, and these effects were counteracted by miR-21-5p inhibitor (P <0.05). miR-21-5p overexpression also contributed to TGF-[beta]1-induced a-SMA, CoL1A1 and TIMP-1 expression in LX2 cells (P <0.05). Co-culture with HBV-infected NTCP-Huh7.5.1 cells upregu lated TGF-[beta]1/miR-21-5p activity and CoL1A1 expression in LX2 cells compared with normal control, which were significantly reduced by miR-21-5p inhibitor (P <0.05). miR-21-5p levels were significantly correlated with the liver fibrosis score (r=0.888; P <0.05). These data demonstrated that HBV induced liver fibrosis via the TGF-[beta]1/miR-21-5p pathway and suggested that miR-21-5p may be an effective anti-fibrosis target. Key words: hepatitis B virus, liver fibrosis, microRNA-21-5p, TGF-[beta]1</description><subject>Cellular signal transduction</subject><subject>Complications and side effects</subject><subject>Development and progression</subject><subject>Fibrosis</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Hepatitis B</subject><subject>Liver diseases</subject><subject>MicroRNA</subject><subject>Transforming growth factors</subject><issn>1792-0981</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjMFLwzAYxXNQcMwdvQc8p0u-JE1ycw63CQNBiheRkaTJFlnb0dSJ_70FPXjwvcODx-89hG4YLbg2MA9DUwAFWpiS0gs0YcoAoUazKzTL-Z2OkiXTWk7Q3eb-Bae2_vAh42M6hx7H5Poup4zPyeLhEHC1XpFXFwb7xuZNeibAiDzhkx0On_brGl1Ge8xh9ptTVK0equWGbJ_Wj8vFluxLBcSp2tGago6cCvCgFGdSmjpazz0IQbkRXIgSau-FdcE7oWTJQUuIwqjIp-j253Zvj2GX2tgNvfVNyn63KIWREjiDkSr-oUbXoUm-a0NMY_9n8A2mkFZF</recordid><startdate>20210201</startdate><enddate>20210201</enddate><creator>Li, Wenting</creator><creator>Yu, Xiaolan</creator><creator>Chen, Xiliu</creator><creator>Wang, Zheng</creator><creator>Yin, Ming</creator><creator>Zhao, Zonghao</creator><creator>Zhu, Chuanwu</creator><general>Spandidos Publications</general><scope/></search><sort><creationdate>20210201</creationdate><title>HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway</title><author>Li, Wenting ; Yu, Xiaolan ; Chen, Xiliu ; Wang, Zheng ; Yin, Ming ; Zhao, Zonghao ; Zhu, Chuanwu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g672-b7db0d028f3042c27731559dfac3c244039434462dcc4abecb475632852f497f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cellular signal transduction</topic><topic>Complications and side effects</topic><topic>Development and progression</topic><topic>Fibrosis</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Hepatitis B</topic><topic>Liver diseases</topic><topic>MicroRNA</topic><topic>Transforming growth factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Li, Wenting</creatorcontrib><creatorcontrib>Yu, Xiaolan</creatorcontrib><creatorcontrib>Chen, Xiliu</creatorcontrib><creatorcontrib>Wang, Zheng</creatorcontrib><creatorcontrib>Yin, Ming</creatorcontrib><creatorcontrib>Zhao, Zonghao</creatorcontrib><creatorcontrib>Zhu, Chuanwu</creatorcontrib><jtitle>Experimental and therapeutic medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Wenting</au><au>Yu, Xiaolan</au><au>Chen, Xiliu</au><au>Wang, Zheng</au><au>Yin, Ming</au><au>Zhao, Zonghao</au><au>Zhu, Chuanwu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway</atitle><jtitle>Experimental and therapeutic medicine</jtitle><date>2021-02-01</date><risdate>2021</risdate><volume>21</volume><issue>2</issue><issn>1792-0981</issn><abstract>MicroRNA (miR)-21-5p is a newly discovered factor that mediates TGF-[beta]1 signaling. The present study was designed to investigate the role of TGF-[beta]1/miR-21-5p in hepatitis B virus (HBV)-induced liver fibrosis. HBV-infected sodium taurocholate co-transporting polypeptide (NTCP)-transfected Huh7.5.1 cells were co-cultured with LX2 cells to simulate HBV infection in the present study. A total of 29 patients with chronic HBV infection were enrolled. Cells were transfected with miR-21-5p mimic or inhibitor with or without TGF-[beta]1 stimulation. The demographic, biochemical and virological data from the 29 patients were analyzed and liver tissues were collected. miR-21-5p levels and the mRNA and protein expression of a-smooth muscle actin (SMA), collagen type 1 [alpha] 1 (CoL1A1), tissue inhibitor of metalloproteinase (TIMP)-1 and Smad from liver cells or tissues were detected by quantitative PCR analysis and western blotting, respectively. Cell viability was observed, and the liver fibrosis score was evaluated. The association between miR-21-5p and liver fibrosis was evaluated by correlation analysis. HBV infection upregulated TGF-[beta]1/miR-21-5p mRNA expression in NTCP-Huh7.5.1 cells compared with mock infection (P <0.05). TGF-[beta]1 incubation significantly increased miR-21-5p levels, as well as the mRNA and protein expression of [alpha]-SMA, CoL1A1 and TIMP-1, and reduced Smad7 expression in LX2 cells compared with the normal group, and these effects were counteracted by miR-21-5p inhibitor (P <0.05). miR-21-5p overexpression also contributed to TGF-[beta]1-induced a-SMA, CoL1A1 and TIMP-1 expression in LX2 cells (P <0.05). Co-culture with HBV-infected NTCP-Huh7.5.1 cells upregu lated TGF-[beta]1/miR-21-5p activity and CoL1A1 expression in LX2 cells compared with normal control, which were significantly reduced by miR-21-5p inhibitor (P <0.05). miR-21-5p levels were significantly correlated with the liver fibrosis score (r=0.888; P <0.05). These data demonstrated that HBV induced liver fibrosis via the TGF-[beta]1/miR-21-5p pathway and suggested that miR-21-5p may be an effective anti-fibrosis target. Key words: hepatitis B virus, liver fibrosis, microRNA-21-5p, TGF-[beta]1</abstract><pub>Spandidos Publications</pub><doi>10.3892/etm.2020.9600</doi></addata></record> |
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| subjects | Cellular signal transduction Complications and side effects Development and progression Fibrosis Genetic aspects Health aspects Hepatitis B Liver diseases MicroRNA Transforming growth factors |
| title | HBV induces liver fibrosis via the TGF-[beta]1/miR-21-5p pathway |
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