Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study
Abstract Background: Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction. Aims and Objectives: The objective of the present study was to eval...
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| Veröffentlicht in: | European journal of general dentistry 2020-05, Vol.9 (2), p.79-83 |
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| container_title | European journal of general dentistry |
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| creator | Silva, Paulo Goberlânio Barros Terto, Caroline Nágila Do Nascimento Andrade, Rebeca Da Cunha Fernandes, Argus Ramom Rolim Rolim, Juliana Paiva Marques Lima Neri, Jiovanne Rabelo |
| description | Abstract
Background:
Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction.
Aims and Objectives:
The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively.
Materials and Methods:
Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an
in vitro
cariogenic challenge, inoculated with
Streptococcus mutans
for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (
n
= 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (
P
|
| doi_str_mv | 10.4103/ejgd.ejgd_137_19 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_cross</sourceid><recordid>TN_cdi_gale_infotracmisc_A623565063</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A623565063</galeid><sourcerecordid>A623565063</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354r-b854be75bb71772bddca72ae77b4f5cbe00c9912f3d8dc9a125bdcec37f2aaba3</originalsourceid><addsrcrecordid>eNp1UU1r3DAUNKWFhjT3HgWFnuKNLNnWurcl9CMQ6KU9C308r7WxpUWSd9lf07_a524CCTQIJL1hZiRmiuJjRVd1RfkN7LZ2tWyy4kJW3ZvignFGy1o0_C3emViXXcva98VVSjtKKYr4uqMXxZ-Nz25yJgbt1EjgoMZZZRf8NbGwjQAk9CSFcdZudPl0TZS35KgyRETjfmEujNm7A8SEDhZ8xkPZARJixHmDvBBRgkKXBwJ7t1XjGAxCZnC-5OUy4_SFbDy58-XB5RhIyrM9fSje9WpMcPV4Xha_v339dfujvP_5_e52c18a3tSx1Oum1iAarUUlBNPWGiWYAiF03TdGA6Wm6yrWc7u2plMVa7Q1YLjomVJa8cvi09kXvwLS-T7kqMzkkpGblvGmbWjLkbX6DwuXBcwweOgd4i8En58JBlBjHpY0l9zSSyI9E7GJlCL0ch_dpOJJVlQuHct_9T7rGCWbs-QYRuwjPYzzEaKcwD74cHxVJ0Unn0pHj5uzRx4cTCB3YY4ec3791b-ob8bt</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Thieme Connect Journals Open Access</source><creator>Silva, Paulo Goberlânio Barros ; Terto, Caroline Nágila Do Nascimento ; Andrade, Rebeca Da Cunha ; Fernandes, Argus Ramom Rolim ; Rolim, Juliana Paiva Marques Lima ; Neri, Jiovanne Rabelo</creator><creatorcontrib>Silva, Paulo Goberlânio Barros ; Terto, Caroline Nágila Do Nascimento ; Andrade, Rebeca Da Cunha ; Fernandes, Argus Ramom Rolim ; Rolim, Juliana Paiva Marques Lima ; Neri, Jiovanne Rabelo</creatorcontrib><description>Abstract
Background:
Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction.
Aims and Objectives:
The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively.
Materials and Methods:
Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an
in vitro
cariogenic challenge, inoculated with
Streptococcus mutans
for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (
n
= 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (
P
< 0.05). ANOVA was used to evaluate WS and solubility, and comparisons were made by
post hoc
analysis by the Student–Newman–Keuls method (
P
< 0.05).
Results:
A statistical difference was observed regarding the antimicrobial potential between the groups without and with EGCG at 0.5% (
P
= 0.03). EGCG 0.5% presented the highest values of solubility and WS (
P
< 0.01 and
P
= 0.009, respectively).
Conclusion:
The addition of 0.5% EGCG was capable of inhibiting biofilm formation; however, it caused significant alteration of the solubility and sorption of the adhesive.</description><identifier>ISSN: 2278-9626</identifier><identifier>EISSN: 2320-4753</identifier><identifier>DOI: 10.4103/ejgd.ejgd_137_19</identifier><language>eng</language><publisher>A-12, 2nd Floor, Sector 2, Noida-201301 UP, India: Thieme Medical and Scientific Publishers Pvt. Ltd</publisher><subject>Catechin ; Collagen ; Metronidazole ; Original Article ; Water</subject><ispartof>European journal of general dentistry, 2020-05, Vol.9 (2), p.79-83</ispartof><rights>European Journal of General Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon.</rights><rights>COPYRIGHT 2020 Medknow Publications and Media Pvt. Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354r-b854be75bb71772bddca72ae77b4f5cbe00c9912f3d8dc9a125bdcec37f2aaba3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.4103/ejgd.ejgd_137_19.pdf$$EPDF$$P50$$Gthieme$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.4103/ejgd.ejgd_137_19$$EHTML$$P50$$Gthieme$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,20871,27903,27904,54566,54594</link.rule.ids></links><search><creatorcontrib>Silva, Paulo Goberlânio Barros</creatorcontrib><creatorcontrib>Terto, Caroline Nágila Do Nascimento</creatorcontrib><creatorcontrib>Andrade, Rebeca Da Cunha</creatorcontrib><creatorcontrib>Fernandes, Argus Ramom Rolim</creatorcontrib><creatorcontrib>Rolim, Juliana Paiva Marques Lima</creatorcontrib><creatorcontrib>Neri, Jiovanne Rabelo</creatorcontrib><title>Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study</title><title>European journal of general dentistry</title><description>Abstract
Background:
Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction.
Aims and Objectives:
The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively.
Materials and Methods:
Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an
in vitro
cariogenic challenge, inoculated with
Streptococcus mutans
for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (
n
= 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (
P
< 0.05). ANOVA was used to evaluate WS and solubility, and comparisons were made by
post hoc
analysis by the Student–Newman–Keuls method (
P
< 0.05).
Results:
A statistical difference was observed regarding the antimicrobial potential between the groups without and with EGCG at 0.5% (
P
= 0.03). EGCG 0.5% presented the highest values of solubility and WS (
P
< 0.01 and
P
= 0.009, respectively).
Conclusion:
The addition of 0.5% EGCG was capable of inhibiting biofilm formation; however, it caused significant alteration of the solubility and sorption of the adhesive.</description><subject>Catechin</subject><subject>Collagen</subject><subject>Metronidazole</subject><subject>Original Article</subject><subject>Water</subject><issn>2278-9626</issn><issn>2320-4753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>0U6</sourceid><recordid>eNp1UU1r3DAUNKWFhjT3HgWFnuKNLNnWurcl9CMQ6KU9C308r7WxpUWSd9lf07_a524CCTQIJL1hZiRmiuJjRVd1RfkN7LZ2tWyy4kJW3ZvignFGy1o0_C3emViXXcva98VVSjtKKYr4uqMXxZ-Nz25yJgbt1EjgoMZZZRf8NbGwjQAk9CSFcdZudPl0TZS35KgyRETjfmEujNm7A8SEDhZ8xkPZARJixHmDvBBRgkKXBwJ7t1XjGAxCZnC-5OUy4_SFbDy58-XB5RhIyrM9fSje9WpMcPV4Xha_v339dfujvP_5_e52c18a3tSx1Oum1iAarUUlBNPWGiWYAiF03TdGA6Wm6yrWc7u2plMVa7Q1YLjomVJa8cvi09kXvwLS-T7kqMzkkpGblvGmbWjLkbX6DwuXBcwweOgd4i8En58JBlBjHpY0l9zSSyI9E7GJlCL0ch_dpOJJVlQuHct_9T7rGCWbs-QYRuwjPYzzEaKcwD74cHxVJ0Unn0pHj5uzRx4cTCB3YY4ec3791b-ob8bt</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Silva, Paulo Goberlânio Barros</creator><creator>Terto, Caroline Nágila Do Nascimento</creator><creator>Andrade, Rebeca Da Cunha</creator><creator>Fernandes, Argus Ramom Rolim</creator><creator>Rolim, Juliana Paiva Marques Lima</creator><creator>Neri, Jiovanne Rabelo</creator><general>Thieme Medical and Scientific Publishers Pvt. Ltd</general><general>Wolters Kluwer India Pvt. Ltd</general><general>Medknow Publications and Media Pvt. Ltd</general><scope>0U6</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20200501</creationdate><title>Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study</title><author>Silva, Paulo Goberlânio Barros ; Terto, Caroline Nágila Do Nascimento ; Andrade, Rebeca Da Cunha ; Fernandes, Argus Ramom Rolim ; Rolim, Juliana Paiva Marques Lima ; Neri, Jiovanne Rabelo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354r-b854be75bb71772bddca72ae77b4f5cbe00c9912f3d8dc9a125bdcec37f2aaba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Catechin</topic><topic>Collagen</topic><topic>Metronidazole</topic><topic>Original Article</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Silva, Paulo Goberlânio Barros</creatorcontrib><creatorcontrib>Terto, Caroline Nágila Do Nascimento</creatorcontrib><creatorcontrib>Andrade, Rebeca Da Cunha</creatorcontrib><creatorcontrib>Fernandes, Argus Ramom Rolim</creatorcontrib><creatorcontrib>Rolim, Juliana Paiva Marques Lima</creatorcontrib><creatorcontrib>Neri, Jiovanne Rabelo</creatorcontrib><collection>Thieme Connect Journals Open Access</collection><collection>CrossRef</collection><jtitle>European journal of general dentistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Silva, Paulo Goberlânio Barros</au><au>Terto, Caroline Nágila Do Nascimento</au><au>Andrade, Rebeca Da Cunha</au><au>Fernandes, Argus Ramom Rolim</au><au>Rolim, Juliana Paiva Marques Lima</au><au>Neri, Jiovanne Rabelo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study</atitle><jtitle>European journal of general dentistry</jtitle><date>2020-05-01</date><risdate>2020</risdate><volume>9</volume><issue>2</issue><spage>79</spage><epage>83</epage><pages>79-83</pages><issn>2278-9626</issn><eissn>2320-4753</eissn><abstract>Abstract
Background:
Several strategies have been developed to reduce collagen degradation in the adhesive interface. Epigallocatechin3gallate (EGCG) has the ability to stabilize collagen, and it is effective in microbial reduction.
Aims and Objectives:
The objective of the present study was to evaluate the antimicrobial potential, water sorption (WS), and solubility of a universal adhesive incorporated with EGCG at concentrations of 0.02%, 0.1%, and 0.5%, respectively.
Materials and Methods:
Atotal of 40 specimens of composite resin disks, to which the adhesives were applied, were divided into four groups: control (without EGCG), 0.02%, 0.1%, and 0.5%. The specimens were submitted to an
in vitro
cariogenic challenge, inoculated with
Streptococcus mutans
for 3 days for biofilm formation. The generated biofilm was collected, and the colonyforming units were established. For the solubility test, the specimens using the adhesive were divided into the same groups mentioned previously (
n
= 10). Adhesive models were made following an ISO standard for sorption and solubility tests. Microbiological data were submitted to the analysis of variance (ANOVA), followed by the Tukey test (
P
< 0.05). ANOVA was used to evaluate WS and solubility, and comparisons were made by
post hoc
analysis by the Student–Newman–Keuls method (
P
< 0.05).
Results:
A statistical difference was observed regarding the antimicrobial potential between the groups without and with EGCG at 0.5% (
P
= 0.03). EGCG 0.5% presented the highest values of solubility and WS (
P
< 0.01 and
P
= 0.009, respectively).
Conclusion:
The addition of 0.5% EGCG was capable of inhibiting biofilm formation; however, it caused significant alteration of the solubility and sorption of the adhesive.</abstract><cop>A-12, 2nd Floor, Sector 2, Noida-201301 UP, India</cop><pub>Thieme Medical and Scientific Publishers Pvt. Ltd</pub><doi>10.4103/ejgd.ejgd_137_19</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 2278-9626 |
| ispartof | European journal of general dentistry, 2020-05, Vol.9 (2), p.79-83 |
| issn | 2278-9626 2320-4753 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A623565063 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Thieme Connect Journals Open Access |
| subjects | Catechin Collagen Metronidazole Original Article Water |
| title | Antimicrobial evaluation, degree of solubility, and water sorption of universal dental adhesive incorporated with epigallocatechin-3-gallate: An In-vitro study |
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