Piperlongumine increases the apoptotic effect of doxorubicin and paclitaxel in a cervical cancer cell line
Objective: Piperlongumine (PL) is an alkaloid derived from the edible pepper (Piper longum L) and it has been described to have various biologic activities including anticancer effects. Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate...
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| Veröffentlicht in: | Nigerian journal of clinical practice 2020-03, Vol.23 (3), p.386-391 |
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| container_title | Nigerian journal of clinical practice |
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| creator | Seber, S Sirin, D Yetisyigit, T Bilgen, T |
| description | Objective: Piperlongumine (PL) is an alkaloid derived from the edible pepper (Piper longum L) and it has been described to have various biologic activities including anticancer effects. Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate the effects of PL/doxorubicin and PL/paclitaxel combination therapies on apoptotic cancer cell death. Material and Methods: The cytotoxicity, IC50 doses by MTT assay confirmed by fluorescent imaging, and apoptotic cell rates by Annexin V staining using flow cytometry were determined for PL, doxorubicin, paclitaxel, and for their combinations. Results: It was shown that the PL by itself induced the apoptosis in HeLa cells. PL in combination with doxorubicin and paclitaxel increased apoptotic cell death compared to either chemotherapeutic agent alone. Conclusion: We conclude that the PL inhibits cancer cell growth by inducing apoptosis and has a potential anticancer activity in cervical cancer, especially when combined with doxorubicin and paclitaxel. |
| doi_str_mv | 10.4103/njcp.njcp_80_19 |
| format | Article |
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Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate the effects of PL/doxorubicin and PL/paclitaxel combination therapies on apoptotic cancer cell death. Material and Methods: The cytotoxicity, IC50 doses by MTT assay confirmed by fluorescent imaging, and apoptotic cell rates by Annexin V staining using flow cytometry were determined for PL, doxorubicin, paclitaxel, and for their combinations. Results: It was shown that the PL by itself induced the apoptosis in HeLa cells. PL in combination with doxorubicin and paclitaxel increased apoptotic cell death compared to either chemotherapeutic agent alone. Conclusion: We conclude that the PL inhibits cancer cell growth by inducing apoptosis and has a potential anticancer activity in cervical cancer, especially when combined with doxorubicin and paclitaxel.</description><identifier>ISSN: 1119-3077</identifier><identifier>DOI: 10.4103/njcp.njcp_80_19</identifier><identifier>PMID: 32134040</identifier><language>eng</language><publisher>India: Wolters Kluwer India Pvt. Ltd</publisher><subject>Alkaloids ; Anthracyclines ; Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Biochemistry ; Cancer treatment ; Cell death ; Cervical cancer ; Dioxolanes - pharmacology ; Doxorubicin - pharmacology ; Drug Synergism ; EDTA ; Female ; HeLa Cells ; Humans ; Paclitaxel - pharmacology ; Uterine Cervical Neoplasms</subject><ispartof>Nigerian journal of clinical practice, 2020-03, Vol.23 (3), p.386-391</ispartof><rights>COPYRIGHT 2020 Medknow Publications and Media Pvt. 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Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate the effects of PL/doxorubicin and PL/paclitaxel combination therapies on apoptotic cancer cell death. Material and Methods: The cytotoxicity, IC50 doses by MTT assay confirmed by fluorescent imaging, and apoptotic cell rates by Annexin V staining using flow cytometry were determined for PL, doxorubicin, paclitaxel, and for their combinations. Results: It was shown that the PL by itself induced the apoptosis in HeLa cells. PL in combination with doxorubicin and paclitaxel increased apoptotic cell death compared to either chemotherapeutic agent alone. Conclusion: We conclude that the PL inhibits cancer cell growth by inducing apoptosis and has a potential anticancer activity in cervical cancer, especially when combined with doxorubicin and paclitaxel.</description><subject>Alkaloids</subject><subject>Anthracyclines</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biochemistry</subject><subject>Cancer treatment</subject><subject>Cell death</subject><subject>Cervical cancer</subject><subject>Dioxolanes - pharmacology</subject><subject>Doxorubicin - pharmacology</subject><subject>Drug Synergism</subject><subject>EDTA</subject><subject>Female</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Paclitaxel - pharmacology</subject><subject>Uterine Cervical Neoplasms</subject><issn>1119-3077</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtPGzEURr0AlUe77g5ZQmKXYMfzXEaIRyUkumjX1h3PNXHw2CPbQ-Df45BSikRlyb6-Ot-3OYR852xecCbO3VqN8-0lGyZ5u0cOOeftTLC6PiBHMa4Zq1rR8C_kQCy4KFjBDsn6pxkxWO_up8E4pMapgBAx0rRCCqMfk09GUdQaVaJe094_-TB1RhlHwfV0BGVNgie0dLuhCsOjUWCpApfn_LeW2tz9lexrsBG__XmPye-ry18XN7Pbu-sfF8vbmSpK4WYIvBZaQwtlhUW1YLXGGqAVohJ12TRNUfK-a3uoF6JbaNRCdUXZMd0J3aICcUxOd733YFEap30KoAYTlVxWvKrLsmybTM0_ofLpcTDKO9Qm7z8Ezv4JrBBsWkVvp2S8ix_B8x2ogo8xoJZjMAOEZ8mZ3JqSr5LeTeXEyS4xTt2A_V_-TVMGljtg423CEB_stMEgM_vg_OZ_vVI0lXzzK14AUDerSA</recordid><startdate>20200301</startdate><enddate>20200301</enddate><creator>Seber, S</creator><creator>Sirin, D</creator><creator>Yetisyigit, T</creator><creator>Bilgen, T</creator><general>Wolters Kluwer India Pvt. 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Our aim in this study was to assess the cytotoxic role of PL on a cervical cancer cell line (HeLa) and to evaluate the effects of PL/doxorubicin and PL/paclitaxel combination therapies on apoptotic cancer cell death. Material and Methods: The cytotoxicity, IC50 doses by MTT assay confirmed by fluorescent imaging, and apoptotic cell rates by Annexin V staining using flow cytometry were determined for PL, doxorubicin, paclitaxel, and for their combinations. Results: It was shown that the PL by itself induced the apoptosis in HeLa cells. PL in combination with doxorubicin and paclitaxel increased apoptotic cell death compared to either chemotherapeutic agent alone. Conclusion: We conclude that the PL inhibits cancer cell growth by inducing apoptosis and has a potential anticancer activity in cervical cancer, especially when combined with doxorubicin and paclitaxel.</abstract><cop>India</cop><pub>Wolters Kluwer India Pvt. 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| ispartof | Nigerian journal of clinical practice, 2020-03, Vol.23 (3), p.386-391 |
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| language | eng |
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| source | African Journals Online (Open Access); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Alkaloids Anthracyclines Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects Biochemistry Cancer treatment Cell death Cervical cancer Dioxolanes - pharmacology Doxorubicin - pharmacology Drug Synergism EDTA Female HeLa Cells Humans Paclitaxel - pharmacology Uterine Cervical Neoplasms |
| title | Piperlongumine increases the apoptotic effect of doxorubicin and paclitaxel in a cervical cancer cell line |
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