The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-[beta]1/Smads signaling pathway
Gentianella acuta (G. acuta) is one of the most commonly used herbs in Chinese Mongolian medicine for the treatment of heart disease. Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlyi...
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| Veröffentlicht in: | International journal of molecular medicine 2020-01, Vol.45 (1), p.223 |
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| container_title | International journal of molecular medicine |
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| creator | Yang, Hong-Xia Xu, Geng-Rui Zhang, Chuang Sun, Jia-Huan Zhang, Yue Song, Jun-Na Li, Yun-Feng Liu, Yu Li, Ai-Ying |
| description | Gentianella acuta (G. acuta) is one of the most commonly used herbs in Chinese Mongolian medicine for the treatment of heart disease. Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlying anti-fibrotic mechanism of G. acuta was further elucidated. Histopathological changes in the heart were observed by hematoxylin-eosin, Masson trichrome and wheat germ agglutinin staining. Relevant molecular events were investigated using immunohistochemistry and western blotting. The results revealed that G. acuta caused improvements in myocardial injury and fibrosis. G. acuta also inhibited collagens I and III and [alpha]-smooth muscle actin production in heart tissue. G. acuta downregulated the expression of transforming growth factor [beta]1 (TGF-[beta]1) and notably inhibited the levels of phosphorylation of TGF-[beta] receptors I and II. Furthermore, G. acuta caused downregulation of the intracellular mothers against decapentaplegic homolog (Smads)2 and 4 expression and inhibited Smads2 and 3 phosphorylation. The results further demonstrated that the mechanism underlying anti-myocardial fibrosis effects of G. acuta was based upon the suppression of the TGF-[beta]1/Smads signaling pathway. Therefore, G. acuta may be a potential therapeutic agent for ameliorating myocardial fibrosis. Key words: Gentianella acuta, isoproterenol, myocardial fibrosis, transforming growth factor-[beta]1, transforming growth factor-[beta]1/mothers against decapentaplegic homolog signaling pathway |
| doi_str_mv | 10.3892/ijmm.2019.4410 |
| format | Article |
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Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlying anti-fibrotic mechanism of G. acuta was further elucidated. Histopathological changes in the heart were observed by hematoxylin-eosin, Masson trichrome and wheat germ agglutinin staining. Relevant molecular events were investigated using immunohistochemistry and western blotting. The results revealed that G. acuta caused improvements in myocardial injury and fibrosis. G. acuta also inhibited collagens I and III and [alpha]-smooth muscle actin production in heart tissue. G. acuta downregulated the expression of transforming growth factor [beta]1 (TGF-[beta]1) and notably inhibited the levels of phosphorylation of TGF-[beta] receptors I and II. Furthermore, G. acuta caused downregulation of the intracellular mothers against decapentaplegic homolog (Smads)2 and 4 expression and inhibited Smads2 and 3 phosphorylation. The results further demonstrated that the mechanism underlying anti-myocardial fibrosis effects of G. acuta was based upon the suppression of the TGF-[beta]1/Smads signaling pathway. Therefore, G. acuta may be a potential therapeutic agent for ameliorating myocardial fibrosis. Key words: Gentianella acuta, isoproterenol, myocardial fibrosis, transforming growth factor-[beta]1, transforming growth factor-[beta]1/mothers against decapentaplegic homolog signaling pathway</description><identifier>ISSN: 1107-3756</identifier><identifier>DOI: 10.3892/ijmm.2019.4410</identifier><language>eng</language><publisher>Spandidos Publications</publisher><subject>Actin ; Bone morphogenetic proteins ; Cardiology ; Cardiovascular diseases ; Fibrosis ; Heart ; Heart diseases ; Immunohistochemistry ; Muscle proteins ; Smooth muscle ; Transforming growth factors</subject><ispartof>International journal of molecular medicine, 2020-01, Vol.45 (1), p.223</ispartof><rights>COPYRIGHT 2020 Spandidos Publications</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Yang, Hong-Xia</creatorcontrib><creatorcontrib>Xu, Geng-Rui</creatorcontrib><creatorcontrib>Zhang, Chuang</creatorcontrib><creatorcontrib>Sun, Jia-Huan</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Song, Jun-Na</creatorcontrib><creatorcontrib>Li, Yun-Feng</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>Li, Ai-Ying</creatorcontrib><title>The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-[beta]1/Smads signaling pathway</title><title>International journal of molecular medicine</title><description>Gentianella acuta (G. acuta) is one of the most commonly used herbs in Chinese Mongolian medicine for the treatment of heart disease. Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlying anti-fibrotic mechanism of G. acuta was further elucidated. Histopathological changes in the heart were observed by hematoxylin-eosin, Masson trichrome and wheat germ agglutinin staining. Relevant molecular events were investigated using immunohistochemistry and western blotting. The results revealed that G. acuta caused improvements in myocardial injury and fibrosis. G. acuta also inhibited collagens I and III and [alpha]-smooth muscle actin production in heart tissue. G. acuta downregulated the expression of transforming growth factor [beta]1 (TGF-[beta]1) and notably inhibited the levels of phosphorylation of TGF-[beta] receptors I and II. Furthermore, G. acuta caused downregulation of the intracellular mothers against decapentaplegic homolog (Smads)2 and 4 expression and inhibited Smads2 and 3 phosphorylation. The results further demonstrated that the mechanism underlying anti-myocardial fibrosis effects of G. acuta was based upon the suppression of the TGF-[beta]1/Smads signaling pathway. Therefore, G. acuta may be a potential therapeutic agent for ameliorating myocardial fibrosis. Key words: Gentianella acuta, isoproterenol, myocardial fibrosis, transforming growth factor-[beta]1, transforming growth factor-[beta]1/mothers against decapentaplegic homolog signaling pathway</description><subject>Actin</subject><subject>Bone morphogenetic proteins</subject><subject>Cardiology</subject><subject>Cardiovascular diseases</subject><subject>Fibrosis</subject><subject>Heart</subject><subject>Heart diseases</subject><subject>Immunohistochemistry</subject><subject>Muscle proteins</subject><subject>Smooth muscle</subject><subject>Transforming growth factors</subject><issn>1107-3756</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjz9PwzAQxTOARCmszJaYk8b577GqaEGqxEA2hKqLfU6vSuwSu4V-EL4vqWBgQDe809PvvdMFwR2Po7QSyYx2fR8lMRdRlvH4IphwHpdhWubFVXDt3C6OkzwT1ST4qrfI4P2A9uAYfvoBpGdWsxUaT2Cw64CBPHhg1O8He0THyNlx8zigsV1IRh0kKtafrIRBEXRMUzNYR44daYyZLTXkyZpzrR-v1atl-Nqghzc-e-lBOeaoNdCRadke_PYDTjfBpYbO4e2vToN6-VAvHsP18-ppMV-HbVGKUKYoBeR5VcqcVymigkKKpGp4o0qR66ZQVZEI1FokIpOghFYJ11KCLLKyEek0uP-pbaHDDRltz__35ORmXsQir_IsO1PRP9Q4CnuS1qCm0f8T-AYHkHpj</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Yang, Hong-Xia</creator><creator>Xu, Geng-Rui</creator><creator>Zhang, Chuang</creator><creator>Sun, Jia-Huan</creator><creator>Zhang, Yue</creator><creator>Song, Jun-Na</creator><creator>Li, Yun-Feng</creator><creator>Liu, Yu</creator><creator>Li, Ai-Ying</creator><general>Spandidos Publications</general><scope/></search><sort><creationdate>20200101</creationdate><title>The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-[beta]1/Smads signaling pathway</title><author>Yang, Hong-Xia ; Xu, Geng-Rui ; Zhang, Chuang ; Sun, Jia-Huan ; Zhang, Yue ; Song, Jun-Na ; Li, Yun-Feng ; Liu, Yu ; Li, Ai-Ying</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g679-c3ec9a5587c5183eeda6c928b1bd795fb6d8629eff9294cad9fd21fccac647b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Actin</topic><topic>Bone morphogenetic proteins</topic><topic>Cardiology</topic><topic>Cardiovascular diseases</topic><topic>Fibrosis</topic><topic>Heart</topic><topic>Heart diseases</topic><topic>Immunohistochemistry</topic><topic>Muscle proteins</topic><topic>Smooth muscle</topic><topic>Transforming growth factors</topic><toplevel>online_resources</toplevel><creatorcontrib>Yang, Hong-Xia</creatorcontrib><creatorcontrib>Xu, Geng-Rui</creatorcontrib><creatorcontrib>Zhang, Chuang</creatorcontrib><creatorcontrib>Sun, Jia-Huan</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Song, Jun-Na</creatorcontrib><creatorcontrib>Li, Yun-Feng</creatorcontrib><creatorcontrib>Liu, Yu</creatorcontrib><creatorcontrib>Li, Ai-Ying</creatorcontrib><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Hong-Xia</au><au>Xu, Geng-Rui</au><au>Zhang, Chuang</au><au>Sun, Jia-Huan</au><au>Zhang, Yue</au><au>Song, Jun-Na</au><au>Li, Yun-Feng</au><au>Liu, Yu</au><au>Li, Ai-Ying</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-[beta]1/Smads signaling pathway</atitle><jtitle>International journal of molecular medicine</jtitle><date>2020-01-01</date><risdate>2020</risdate><volume>45</volume><issue>1</issue><spage>223</spage><pages>223-</pages><issn>1107-3756</issn><abstract>Gentianella acuta (G. acuta) is one of the most commonly used herbs in Chinese Mongolian medicine for the treatment of heart disease. Previously, it was found that G. acuta ameliorated cardiac function and inhibited isoproterenol (ISO)-induced myocardial fibrosis in rats. In this study, the underlying anti-fibrotic mechanism of G. acuta was further elucidated. Histopathological changes in the heart were observed by hematoxylin-eosin, Masson trichrome and wheat germ agglutinin staining. Relevant molecular events were investigated using immunohistochemistry and western blotting. The results revealed that G. acuta caused improvements in myocardial injury and fibrosis. G. acuta also inhibited collagens I and III and [alpha]-smooth muscle actin production in heart tissue. G. acuta downregulated the expression of transforming growth factor [beta]1 (TGF-[beta]1) and notably inhibited the levels of phosphorylation of TGF-[beta] receptors I and II. Furthermore, G. acuta caused downregulation of the intracellular mothers against decapentaplegic homolog (Smads)2 and 4 expression and inhibited Smads2 and 3 phosphorylation. The results further demonstrated that the mechanism underlying anti-myocardial fibrosis effects of G. acuta was based upon the suppression of the TGF-[beta]1/Smads signaling pathway. Therefore, G. acuta may be a potential therapeutic agent for ameliorating myocardial fibrosis. Key words: Gentianella acuta, isoproterenol, myocardial fibrosis, transforming growth factor-[beta]1, transforming growth factor-[beta]1/mothers against decapentaplegic homolog signaling pathway</abstract><pub>Spandidos Publications</pub><doi>10.3892/ijmm.2019.4410</doi></addata></record> |
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| source | Spandidos Publications Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Actin Bone morphogenetic proteins Cardiology Cardiovascular diseases Fibrosis Heart Heart diseases Immunohistochemistry Muscle proteins Smooth muscle Transforming growth factors |
| title | The aqueous extract of Gentianella acuta improves isoproterenol-induced myocardial fibrosis via inhibition of the TGF-[beta]1/Smads signaling pathway |
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