Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer

Background: The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in l...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:OncoTargets and therapy 2019-11, p.9017
Hauptverfasser: Jin, Yuxing, Xie, Huikang, Duan, Liang, Zhao, Deping, Ding, Jiaan, Jiang, Gening
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page 9017
container_title OncoTargets and therapy
container_volume
creator Jin, Yuxing
Xie, Huikang
Duan, Liang
Zhao, Deping
Ding, Jiaan
Jiang, Gening
description Background: The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progression. Methods: Expression of CASC9 was determined in lung cancer tissues and cell lines by real-time PCR. CCK-8, colony formation, wound healing and transwell assays were done to evaluate the cell proliferation, migration and invasion capacities in vitro. Real-time PCR, Western blot and RNA immunoprecipitation (RIP) assays were performed to dissect the mechanisms. Results: CASC9 was overexpressed in lung cancer specimens and cell lines. Knockdown of CASC9 inhibited cell proliferation, migration, invasion and EMT in lung cancer cells. While overexpression of CASC9 in normal lung epithelial cells did the opposite. CASC9 interacted with HIF-1[alpha] and enhanced its protein stability. They formed a positive feedback loop by reciprocally inducing each other expression and regulated cell proliferation and metastasis. Conclusion: Our findings demonstrated a novel regulatory signaling pathway, namely the CASC9/HIF-1[alpha] axis, which was involved in lung cancer progression. These findings can provide valuable insights on the potential therapy application for lung cancer. Keywords: CASC9, HIF-1 [alpha], cell proliferation, metastasis, lung cancer
doi_str_mv 10.2147/OTT.S226078
format Article
fullrecord <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A609010034</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A609010034</galeid><sourcerecordid>A609010034</sourcerecordid><originalsourceid>FETCH-LOGICAL-g984-1b37bc826ad9f807562676a5fe92c35063ae170493c708b0b5777a9f72011173</originalsourceid><addsrcrecordid>eNptUN1KwzAUDqLgnF75AgHBu84kbZPmshTrBnUbrnci4zRNt2jWjKbzFXxt68_FBDkHzsfh--EchK4pmTAaibtFWU5WjHEikhM0olQkAZchOT3C5-jC-1dCOE9YNEIfhWs3eO7aIHO1GeDTPMVZusokTtsaT2d5QJ_B7rfwgnPX7XCKl86b3rxrnGtdV6DecOHcHpcO56CMNT30GmfaWrzsnDWN7qA3rv32e9Q9-KGNx7MWF4chMINW6e4SnTVgvb76nWO0yu_LbBoUi4dZlhbBRiZRQKtQVCphHGrZJETEnHHBIW60ZCqMCQ9BU0EiGSpBkopUsRACZCMYocMHwjG6-XHdgNVr0zau70DtjFfrlBNJKCFhNLAm_7CGqvXOKNfqxgz7P4LbI8FWg-233tnD19n-mPgJ2YR7BQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer</title><source>Dove Press Free</source><source>PubMed Central Open Access</source><source>Access via Taylor &amp; Francis (Open Access Collection)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Jin, Yuxing ; Xie, Huikang ; Duan, Liang ; Zhao, Deping ; Ding, Jiaan ; Jiang, Gening</creator><creatorcontrib>Jin, Yuxing ; Xie, Huikang ; Duan, Liang ; Zhao, Deping ; Ding, Jiaan ; Jiang, Gening</creatorcontrib><description>Background: The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progression. Methods: Expression of CASC9 was determined in lung cancer tissues and cell lines by real-time PCR. CCK-8, colony formation, wound healing and transwell assays were done to evaluate the cell proliferation, migration and invasion capacities in vitro. Real-time PCR, Western blot and RNA immunoprecipitation (RIP) assays were performed to dissect the mechanisms. Results: CASC9 was overexpressed in lung cancer specimens and cell lines. Knockdown of CASC9 inhibited cell proliferation, migration, invasion and EMT in lung cancer cells. While overexpression of CASC9 in normal lung epithelial cells did the opposite. CASC9 interacted with HIF-1[alpha] and enhanced its protein stability. They formed a positive feedback loop by reciprocally inducing each other expression and regulated cell proliferation and metastasis. Conclusion: Our findings demonstrated a novel regulatory signaling pathway, namely the CASC9/HIF-1[alpha] axis, which was involved in lung cancer progression. These findings can provide valuable insights on the potential therapy application for lung cancer. Keywords: CASC9, HIF-1 [alpha], cell proliferation, metastasis, lung cancer</description><identifier>ISSN: 1178-6930</identifier><identifier>EISSN: 1178-6930</identifier><identifier>DOI: 10.2147/OTT.S226078</identifier><language>eng</language><publisher>Dove Medical Press Limited</publisher><subject>Antisense RNA ; Cancer cells ; Cancer metastasis ; Cancer research ; Development and progression ; Disease susceptibility ; Health aspects ; Lung cancer ; Medical schools ; Novels ; RNA ; Wound care ; Wound healing</subject><ispartof>OncoTargets and therapy, 2019-11, p.9017</ispartof><rights>COPYRIGHT 2019 Dove Medical Press Limited</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Jin, Yuxing</creatorcontrib><creatorcontrib>Xie, Huikang</creatorcontrib><creatorcontrib>Duan, Liang</creatorcontrib><creatorcontrib>Zhao, Deping</creatorcontrib><creatorcontrib>Ding, Jiaan</creatorcontrib><creatorcontrib>Jiang, Gening</creatorcontrib><title>Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer</title><title>OncoTargets and therapy</title><description>Background: The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progression. Methods: Expression of CASC9 was determined in lung cancer tissues and cell lines by real-time PCR. CCK-8, colony formation, wound healing and transwell assays were done to evaluate the cell proliferation, migration and invasion capacities in vitro. Real-time PCR, Western blot and RNA immunoprecipitation (RIP) assays were performed to dissect the mechanisms. Results: CASC9 was overexpressed in lung cancer specimens and cell lines. Knockdown of CASC9 inhibited cell proliferation, migration, invasion and EMT in lung cancer cells. While overexpression of CASC9 in normal lung epithelial cells did the opposite. CASC9 interacted with HIF-1[alpha] and enhanced its protein stability. They formed a positive feedback loop by reciprocally inducing each other expression and regulated cell proliferation and metastasis. Conclusion: Our findings demonstrated a novel regulatory signaling pathway, namely the CASC9/HIF-1[alpha] axis, which was involved in lung cancer progression. These findings can provide valuable insights on the potential therapy application for lung cancer. Keywords: CASC9, HIF-1 [alpha], cell proliferation, metastasis, lung cancer</description><subject>Antisense RNA</subject><subject>Cancer cells</subject><subject>Cancer metastasis</subject><subject>Cancer research</subject><subject>Development and progression</subject><subject>Disease susceptibility</subject><subject>Health aspects</subject><subject>Lung cancer</subject><subject>Medical schools</subject><subject>Novels</subject><subject>RNA</subject><subject>Wound care</subject><subject>Wound healing</subject><issn>1178-6930</issn><issn>1178-6930</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptUN1KwzAUDqLgnF75AgHBu84kbZPmshTrBnUbrnci4zRNt2jWjKbzFXxt68_FBDkHzsfh--EchK4pmTAaibtFWU5WjHEikhM0olQkAZchOT3C5-jC-1dCOE9YNEIfhWs3eO7aIHO1GeDTPMVZusokTtsaT2d5QJ_B7rfwgnPX7XCKl86b3rxrnGtdV6DecOHcHpcO56CMNT30GmfaWrzsnDWN7qA3rv32e9Q9-KGNx7MWF4chMINW6e4SnTVgvb76nWO0yu_LbBoUi4dZlhbBRiZRQKtQVCphHGrZJETEnHHBIW60ZCqMCQ9BU0EiGSpBkopUsRACZCMYocMHwjG6-XHdgNVr0zau70DtjFfrlBNJKCFhNLAm_7CGqvXOKNfqxgz7P4LbI8FWg-233tnD19n-mPgJ2YR7BQ</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Jin, Yuxing</creator><creator>Xie, Huikang</creator><creator>Duan, Liang</creator><creator>Zhao, Deping</creator><creator>Ding, Jiaan</creator><creator>Jiang, Gening</creator><general>Dove Medical Press Limited</general><scope/></search><sort><creationdate>20191101</creationdate><title>Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer</title><author>Jin, Yuxing ; Xie, Huikang ; Duan, Liang ; Zhao, Deping ; Ding, Jiaan ; Jiang, Gening</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g984-1b37bc826ad9f807562676a5fe92c35063ae170493c708b0b5777a9f72011173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antisense RNA</topic><topic>Cancer cells</topic><topic>Cancer metastasis</topic><topic>Cancer research</topic><topic>Development and progression</topic><topic>Disease susceptibility</topic><topic>Health aspects</topic><topic>Lung cancer</topic><topic>Medical schools</topic><topic>Novels</topic><topic>RNA</topic><topic>Wound care</topic><topic>Wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Yuxing</creatorcontrib><creatorcontrib>Xie, Huikang</creatorcontrib><creatorcontrib>Duan, Liang</creatorcontrib><creatorcontrib>Zhao, Deping</creatorcontrib><creatorcontrib>Ding, Jiaan</creatorcontrib><creatorcontrib>Jiang, Gening</creatorcontrib><jtitle>OncoTargets and therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Yuxing</au><au>Xie, Huikang</au><au>Duan, Liang</au><au>Zhao, Deping</au><au>Ding, Jiaan</au><au>Jiang, Gening</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer</atitle><jtitle>OncoTargets and therapy</jtitle><date>2019-11-01</date><risdate>2019</risdate><spage>9017</spage><pages>9017-</pages><issn>1178-6930</issn><eissn>1178-6930</eissn><abstract>Background: The long noncoding RNA cancer susceptibility 9 (CASC9) has been recognized as an important modulator of cell growth and metastasis in many cancers. However, its detailed roles in lung cancer remain unclear. In this study, we aimed to investigate its functions and molecular mechanism in lung cancer progression. Methods: Expression of CASC9 was determined in lung cancer tissues and cell lines by real-time PCR. CCK-8, colony formation, wound healing and transwell assays were done to evaluate the cell proliferation, migration and invasion capacities in vitro. Real-time PCR, Western blot and RNA immunoprecipitation (RIP) assays were performed to dissect the mechanisms. Results: CASC9 was overexpressed in lung cancer specimens and cell lines. Knockdown of CASC9 inhibited cell proliferation, migration, invasion and EMT in lung cancer cells. While overexpression of CASC9 in normal lung epithelial cells did the opposite. CASC9 interacted with HIF-1[alpha] and enhanced its protein stability. They formed a positive feedback loop by reciprocally inducing each other expression and regulated cell proliferation and metastasis. Conclusion: Our findings demonstrated a novel regulatory signaling pathway, namely the CASC9/HIF-1[alpha] axis, which was involved in lung cancer progression. These findings can provide valuable insights on the potential therapy application for lung cancer. Keywords: CASC9, HIF-1 [alpha], cell proliferation, metastasis, lung cancer</abstract><pub>Dove Medical Press Limited</pub><doi>10.2147/OTT.S226078</doi></addata></record>
fulltext fulltext
identifier ISSN: 1178-6930
ispartof OncoTargets and therapy, 2019-11, p.9017
issn 1178-6930
1178-6930
language eng
recordid cdi_gale_infotracmisc_A609010034
source Dove Press Free; PubMed Central Open Access; Access via Taylor & Francis (Open Access Collection); EZB-FREE-00999 freely available EZB journals; PubMed Central
subjects Antisense RNA
Cancer cells
Cancer metastasis
Cancer research
Development and progression
Disease susceptibility
Health aspects
Lung cancer
Medical schools
Novels
RNA
Wound care
Wound healing
title Long Non-Coding RNA CASC9 And HIF-1[alpha] Form A Positive Feedback Loop To Facilitate Cell Proliferation And Metastasis In Lung Cancer
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T01%3A55%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Long%20Non-Coding%20RNA%20CASC9%20And%20HIF-1%5Balpha%5D%20Form%20A%20Positive%20Feedback%20Loop%20To%20Facilitate%20Cell%20Proliferation%20And%20Metastasis%20In%20Lung%20Cancer&rft.jtitle=OncoTargets%20and%20therapy&rft.au=Jin,%20Yuxing&rft.date=2019-11-01&rft.spage=9017&rft.pages=9017-&rft.issn=1178-6930&rft.eissn=1178-6930&rft_id=info:doi/10.2147/OTT.S226078&rft_dat=%3Cgale%3EA609010034%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A609010034&rfr_iscdi=true