Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition

When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-[kappa]B pathway activation in some neurons (Kurz et al., 2017). Although we showed that...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:eLife 2019-10, Vol.8
Hauptverfasser: Masuzzo, Ambra, Manière, Gérard, Viallat-Lieutaud, Annelise, Avazeri, Émilie, Zugasti, Olivier, Grosjean, Yaël, Kurz, C Léopold, Royet, Julien
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title eLife
container_volume 8
creator Masuzzo, Ambra
Manière, Gérard
Viallat-Lieutaud, Annelise
Avazeri, Émilie
Zugasti, Olivier
Grosjean, Yaël
Kurz, C Léopold
Royet, Julien
description When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-[kappa]B pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-[kappa]B pathway component. We functionally demonstrated that NF-[kappa]B pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-[kappa]B cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.
doi_str_mv 10.7554/eLife.50559
format Article
fullrecord <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A604132875</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A604132875</galeid><sourcerecordid>A604132875</sourcerecordid><originalsourceid>FETCH-LOGICAL-g1015-2863ddeaed1b6ba036fd3c516b07b35c80503caeb5502c2f5c54bc0bd16cadde3</originalsourceid><addsrcrecordid>eNptj01LAzEQhoMoWGpP_oGAJw9bk93N7vZYi9VCUfEDBJGSj8kS3U2WTVoU_PGm6KEFZw4zDO_zMi9Cp5SMS8byC1gaDWNGGJscoEFKGElIlb8c7uzHaOT9O4lV5lVFJwP0fQ9dMMrVzZfkNlHQgVVgA76dJ68fvOv42yXmMpgND8ZZbCzm2Le8abBfCw8BO41Fz-PdyeA63hoLfW0ktrDunfVYOht613isIWKA3cZ0zput2wk60rzxMPqbQ_Q8v3qa3STLu-vFbLpMakooS9KqyJQCDoqKQnCSFVplktFCkFJkTFYxXiY5CMZIKlPNJMuFJELRQvIIZkN09utbxwdWxmoXei5b4-VqWpCcZmlVsqga_6OKraA1MQVoE-97wPkesE0Kn6Hma-9Xi8eHXe0PlAuBKA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition</title><source>PubMed</source><source>Directory of Open Access Journals</source><source>EZB Electronic Journals Library</source><source>PubMed Central Open Access</source><creator>Masuzzo, Ambra ; Manière, Gérard ; Viallat-Lieutaud, Annelise ; Avazeri, Émilie ; Zugasti, Olivier ; Grosjean, Yaël ; Kurz, C Léopold ; Royet, Julien</creator><creatorcontrib>Masuzzo, Ambra ; Manière, Gérard ; Viallat-Lieutaud, Annelise ; Avazeri, Émilie ; Zugasti, Olivier ; Grosjean, Yaël ; Kurz, C Léopold ; Royet, Julien</creatorcontrib><description>When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-[kappa]B pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-[kappa]B pathway component. We functionally demonstrated that NF-[kappa]B pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-[kappa]B cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.</description><identifier>ISSN: 2050-084X</identifier><identifier>EISSN: 2050-084X</identifier><identifier>DOI: 10.7554/eLife.50559</identifier><language>eng</language><publisher>eLife Science Publications, Ltd</publisher><subject>Animal behavior ; Brain ; Brain research ; Drosophila ; Infection ; Microorganisms ; Neurons ; Oviposition ; Peptidoglycans ; Physiological aspects ; Women ; Zoological research</subject><ispartof>eLife, 2019-10, Vol.8</ispartof><rights>COPYRIGHT 2019 eLife Science Publications, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27922,27923</link.rule.ids></links><search><creatorcontrib>Masuzzo, Ambra</creatorcontrib><creatorcontrib>Manière, Gérard</creatorcontrib><creatorcontrib>Viallat-Lieutaud, Annelise</creatorcontrib><creatorcontrib>Avazeri, Émilie</creatorcontrib><creatorcontrib>Zugasti, Olivier</creatorcontrib><creatorcontrib>Grosjean, Yaël</creatorcontrib><creatorcontrib>Kurz, C Léopold</creatorcontrib><creatorcontrib>Royet, Julien</creatorcontrib><title>Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition</title><title>eLife</title><description>When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-[kappa]B pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-[kappa]B pathway component. We functionally demonstrated that NF-[kappa]B pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-[kappa]B cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.</description><subject>Animal behavior</subject><subject>Brain</subject><subject>Brain research</subject><subject>Drosophila</subject><subject>Infection</subject><subject>Microorganisms</subject><subject>Neurons</subject><subject>Oviposition</subject><subject>Peptidoglycans</subject><subject>Physiological aspects</subject><subject>Women</subject><subject>Zoological research</subject><issn>2050-084X</issn><issn>2050-084X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNptj01LAzEQhoMoWGpP_oGAJw9bk93N7vZYi9VCUfEDBJGSj8kS3U2WTVoU_PGm6KEFZw4zDO_zMi9Cp5SMS8byC1gaDWNGGJscoEFKGElIlb8c7uzHaOT9O4lV5lVFJwP0fQ9dMMrVzZfkNlHQgVVgA76dJ68fvOv42yXmMpgND8ZZbCzm2Le8abBfCw8BO41Fz-PdyeA63hoLfW0ktrDunfVYOht613isIWKA3cZ0zput2wk60rzxMPqbQ_Q8v3qa3STLu-vFbLpMakooS9KqyJQCDoqKQnCSFVplktFCkFJkTFYxXiY5CMZIKlPNJMuFJELRQvIIZkN09utbxwdWxmoXei5b4-VqWpCcZmlVsqga_6OKraA1MQVoE-97wPkesE0Kn6Hma-9Xi8eHXe0PlAuBKA</recordid><startdate>20191029</startdate><enddate>20191029</enddate><creator>Masuzzo, Ambra</creator><creator>Manière, Gérard</creator><creator>Viallat-Lieutaud, Annelise</creator><creator>Avazeri, Émilie</creator><creator>Zugasti, Olivier</creator><creator>Grosjean, Yaël</creator><creator>Kurz, C Léopold</creator><creator>Royet, Julien</creator><general>eLife Science Publications, Ltd</general><scope>ISR</scope></search><sort><creationdate>20191029</creationdate><title>Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition</title><author>Masuzzo, Ambra ; Manière, Gérard ; Viallat-Lieutaud, Annelise ; Avazeri, Émilie ; Zugasti, Olivier ; Grosjean, Yaël ; Kurz, C Léopold ; Royet, Julien</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1015-2863ddeaed1b6ba036fd3c516b07b35c80503caeb5502c2f5c54bc0bd16cadde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal behavior</topic><topic>Brain</topic><topic>Brain research</topic><topic>Drosophila</topic><topic>Infection</topic><topic>Microorganisms</topic><topic>Neurons</topic><topic>Oviposition</topic><topic>Peptidoglycans</topic><topic>Physiological aspects</topic><topic>Women</topic><topic>Zoological research</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masuzzo, Ambra</creatorcontrib><creatorcontrib>Manière, Gérard</creatorcontrib><creatorcontrib>Viallat-Lieutaud, Annelise</creatorcontrib><creatorcontrib>Avazeri, Émilie</creatorcontrib><creatorcontrib>Zugasti, Olivier</creatorcontrib><creatorcontrib>Grosjean, Yaël</creatorcontrib><creatorcontrib>Kurz, C Léopold</creatorcontrib><creatorcontrib>Royet, Julien</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>eLife</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masuzzo, Ambra</au><au>Manière, Gérard</au><au>Viallat-Lieutaud, Annelise</au><au>Avazeri, Émilie</au><au>Zugasti, Olivier</au><au>Grosjean, Yaël</au><au>Kurz, C Léopold</au><au>Royet, Julien</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition</atitle><jtitle>eLife</jtitle><date>2019-10-29</date><risdate>2019</risdate><volume>8</volume><issn>2050-084X</issn><eissn>2050-084X</eissn><abstract>When facing microbes, animals engage in behaviors that lower the impact of the infection. We previously demonstrated that internal sensing of bacterial peptidoglycan reduces Drosophila female oviposition via NF-[kappa]B pathway activation in some neurons (Kurz et al., 2017). Although we showed that the neuromodulator octopamine is implicated, the identity of the involved neurons, as well as the physiological mechanism blocking egg-laying, remained unknown. In this study, we identified few ventral nerve cord and brain octopaminergic neurons expressing an NF-[kappa]B pathway component. We functionally demonstrated that NF-[kappa]B pathway activation in the brain, but not in the ventral nerve cord octopaminergic neurons, triggers an egg-laying drop in response to infection. Furthermore, we demonstrated via calcium imaging that the activity of these neurons can be directly modulated by peptidoglycan and that these cells do not control other octopamine-dependent behaviors such as female receptivity. This study shows that by sensing peptidoglycan and hence activating NF-[kappa]B cascade, a couple of brain neurons modulate a specific octopamine-dependent behavior to adapt female physiology status to their infectious state.</abstract><pub>eLife Science Publications, Ltd</pub><doi>10.7554/eLife.50559</doi></addata></record>
fulltext fulltext
identifier ISSN: 2050-084X
ispartof eLife, 2019-10, Vol.8
issn 2050-084X
2050-084X
language eng
recordid cdi_gale_infotracmisc_A604132875
source PubMed; Directory of Open Access Journals; EZB Electronic Journals Library; PubMed Central Open Access
subjects Animal behavior
Brain
Brain research
Drosophila
Infection
Microorganisms
Neurons
Oviposition
Peptidoglycans
Physiological aspects
Women
Zoological research
title Peptidoglycan-dependent NF-[kappa]B activation in a small subset of brain octopaminergic neurons controls female oviposition
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T15%3A59%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Peptidoglycan-dependent%20NF-%5Bkappa%5DB%20activation%20in%20a%20small%20subset%20of%20brain%20octopaminergic%20neurons%20controls%20female%20oviposition&rft.jtitle=eLife&rft.au=Masuzzo,%20Ambra&rft.date=2019-10-29&rft.volume=8&rft.issn=2050-084X&rft.eissn=2050-084X&rft_id=info:doi/10.7554/eLife.50559&rft_dat=%3Cgale%3EA604132875%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A604132875&rfr_iscdi=true