Antimicrobial Effectiveness of Bioactive Phenazine Against Clinical Isolates

The bioactive phenazine extracted from Pseudomonas aeruginosa were obtained from the clinical isolates collected from different pathological laboratories of Karachi, Pakistan and its antimicrobial activity was assayed against pathogenic microorganisms. It was found that various gram negative and gram positive bacteria and some species of Candida shown to be sensitive against pyocyanin. Extraction was carried out by using chloroform and agar well diffusion protocol was performed for its antimicrobial effectiveness. The maximum average zone of inhibition observed by 20 p.L of purified pyocyanin was 16 mm that was against Proteus sp. in case of bacteria and 17 mm against Candida sp. Keywords: phenazine, Pseudomonas aeruginosa, antimicrobial activity, pyocyanin