Decellularization of Human Umbilical Arteries/ Descelularizacion de las Arterias Umbilicales Humanas
Arterial obstruction in small diameter (
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| Veröffentlicht in: | International journal of morphology 2019-03, Vol.37 (1), p.111 |
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| creator | Rodriguez-Rodriguez, Victor Emanuel Quiroga-Garza, Alejandro Rodriguez-Roque, Carlos Saul de Jesus Loera-Arias, Maria Soto-Dominguez, Adolfo Guzman-Lopez, Santos Vilchez-Cavazos, Jose Felix Montes-de-Oca-Luna, Roberto Elizondo- Omana, Rodrigo Enrique |
| description | Arterial obstruction in small diameter ( |
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Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM. KEY WORDS: Decellularization; Umbilical artery; Vascular graft; Extracellular matrix. RESUMEN: la obstruccion arterial en vasos de pequeno diametro (<6 mm) se trata muchas veces con injertos, sin embargo, los autologos no siempre estan disponibles y los sinteticos tienen una alta tasa de complicaciones. La descelularizacion de las arterias umbilicales puede proporcionar una solucion, pero el metodo ideal es discutible. Comparamos la efectividad entre los metodos SDS y Triton X-100. Cordones umbilicales obtenidos a partir de embarazos a termino con evolucion normal y sin complicaciones evidentes del recien nacido, se microdiseccionaron en 12 horas y se almacenaron en solucion salina con fosfato sin congelacion. Las arterias se procesaron luego para la descelularizacion usando los protocolos de SDS al 0,1 % y 1 %, y Triton X-100 al 1 %. Se realizo la evaluacion de material celular y nuclear, fibras de colageno, fibras elasticas y glucosoaminoglicanos de la matriz extracelular (MEC), asi como el analisis morfometrico bajo tecnicas histologicas e inmunohistoquimicas. Triton X-100 fue ineficaz, conservando los restos nucleares identificados por inmunofluorescencia, tuvo el dano mas notable a las fibras elasticas y la disminucion del colageno. SDS efectivamente elimino los nucleos y tuvo una disminucion menor en las fibras elasticas y el colageno. Laminina fue preservado en todos los grupos. No se identificaron diferencias significativas en los diametros luminales; sin embargo, la capa media disminuyo debido a la descelularizacion de las celulas musculares. la descelularizacion con SDS al 0,1 % fue la mas efectiva para eliminar celulas y preservar los principales componentes de la MEC. KEY WORDS: Decellularization; Umbilical artery; vascular graft; extracellular matrix</description><identifier>ISSN: 0717-9367</identifier><language>eng</language><publisher>Universidad de La Frontera, Facultad de Medicina</publisher><ispartof>International journal of morphology, 2019-03, Vol.37 (1), p.111</ispartof><rights>COPYRIGHT 2019 Universidad de La Frontera, Facultad de Medicina</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Rodriguez-Rodriguez, Victor Emanuel</creatorcontrib><creatorcontrib>Quiroga-Garza, Alejandro</creatorcontrib><creatorcontrib>Rodriguez-Roque, Carlos Saul</creatorcontrib><creatorcontrib>de Jesus Loera-Arias, Maria</creatorcontrib><creatorcontrib>Soto-Dominguez, Adolfo</creatorcontrib><creatorcontrib>Guzman-Lopez, Santos</creatorcontrib><creatorcontrib>Vilchez-Cavazos, Jose Felix</creatorcontrib><creatorcontrib>Montes-de-Oca-Luna, Roberto</creatorcontrib><creatorcontrib>Elizondo- Omana, Rodrigo Enrique</creatorcontrib><title>Decellularization of Human Umbilical Arteries/ Descelularizacion de las Arterias Umbilicales Humanas</title><title>International journal of morphology</title><description>Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren't always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM. KEY WORDS: Decellularization; Umbilical artery; Vascular graft; Extracellular matrix. RESUMEN: la obstruccion arterial en vasos de pequeno diametro (<6 mm) se trata muchas veces con injertos, sin embargo, los autologos no siempre estan disponibles y los sinteticos tienen una alta tasa de complicaciones. La descelularizacion de las arterias umbilicales puede proporcionar una solucion, pero el metodo ideal es discutible. Comparamos la efectividad entre los metodos SDS y Triton X-100. Cordones umbilicales obtenidos a partir de embarazos a termino con evolucion normal y sin complicaciones evidentes del recien nacido, se microdiseccionaron en 12 horas y se almacenaron en solucion salina con fosfato sin congelacion. Las arterias se procesaron luego para la descelularizacion usando los protocolos de SDS al 0,1 % y 1 %, y Triton X-100 al 1 %. Se realizo la evaluacion de material celular y nuclear, fibras de colageno, fibras elasticas y glucosoaminoglicanos de la matriz extracelular (MEC), asi como el analisis morfometrico bajo tecnicas histologicas e inmunohistoquimicas. Triton X-100 fue ineficaz, conservando los restos nucleares identificados por inmunofluorescencia, tuvo el dano mas notable a las fibras elasticas y la disminucion del colageno. SDS efectivamente elimino los nucleos y tuvo una disminucion menor en las fibras elasticas y el colageno. Laminina fue preservado en todos los grupos. No se identificaron diferencias significativas en los diametros luminales; sin embargo, la capa media disminuyo debido a la descelularizacion de las celulas musculares. la descelularizacion con SDS al 0,1 % fue la mas efectiva para eliminar celulas y preservar los principales componentes de la MEC. 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Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM. KEY WORDS: Decellularization; Umbilical artery; Vascular graft; Extracellular matrix. RESUMEN: la obstruccion arterial en vasos de pequeno diametro (<6 mm) se trata muchas veces con injertos, sin embargo, los autologos no siempre estan disponibles y los sinteticos tienen una alta tasa de complicaciones. La descelularizacion de las arterias umbilicales puede proporcionar una solucion, pero el metodo ideal es discutible. Comparamos la efectividad entre los metodos SDS y Triton X-100. Cordones umbilicales obtenidos a partir de embarazos a termino con evolucion normal y sin complicaciones evidentes del recien nacido, se microdiseccionaron en 12 horas y se almacenaron en solucion salina con fosfato sin congelacion. Las arterias se procesaron luego para la descelularizacion usando los protocolos de SDS al 0,1 % y 1 %, y Triton X-100 al 1 %. Se realizo la evaluacion de material celular y nuclear, fibras de colageno, fibras elasticas y glucosoaminoglicanos de la matriz extracelular (MEC), asi como el analisis morfometrico bajo tecnicas histologicas e inmunohistoquimicas. Triton X-100 fue ineficaz, conservando los restos nucleares identificados por inmunofluorescencia, tuvo el dano mas notable a las fibras elasticas y la disminucion del colageno. SDS efectivamente elimino los nucleos y tuvo una disminucion menor en las fibras elasticas y el colageno. Laminina fue preservado en todos los grupos. No se identificaron diferencias significativas en los diametros luminales; sin embargo, la capa media disminuyo debido a la descelularizacion de las celulas musculares. la descelularizacion con SDS al 0,1 % fue la mas efectiva para eliminar celulas y preservar los principales componentes de la MEC. KEY WORDS: Decellularization; Umbilical artery; vascular graft; extracellular matrix</abstract><pub>Universidad de La Frontera, Facultad de Medicina</pub></addata></record> |
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| title | Decellularization of Human Umbilical Arteries/ Descelularizacion de las Arterias Umbilicales Humanas |
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