Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels
Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tu...
Gespeichert in:
| Veröffentlicht in: | The Eurasian journal of medicine 2018-10, Vol.50 (3), p.156 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | 156 |
| container_title | The Eurasian journal of medicine |
| container_volume | 50 |
| creator | Kilic, Fatma Sultan Kaygisiz, Bilgin Aydin, Sule Yildirim, Cafer Karimkhani, Hadi Oner, Setenay |
| description | Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor necrosis factor-[alpha] (TNF-[alpha]) and interleukine-1 [beta] (IL-1[beta]) acting as acute phase cytokines in inflammation, and anti-inflammatory cytokine IL-10, in rats.Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control group were injected once intraperitoneally prior to administration of 100 [micro]l of 1% CAR into the right hind paw of the rats. The paw thickness was measured using gauge calipers (Vernier calipers) before (0 hour) and every hour afterwards for 6 hours following the inflammation induction. The cytokine levels in the blood serum obtained intracardiacally were determined after 6 hours using the enzyme-linked immunosorbent assay method. p |
| doi_str_mv | 10.5152/eurasianjmed.201.1261 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A564465369</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A564465369</galeid><sourcerecordid>A564465369</sourcerecordid><originalsourceid>FETCH-LOGICAL-g679-6156c38f8a0a1fbb30ae0d4c0137073169b62d9d111337485648331da62d2f013</originalsourceid><addsrcrecordid>eNptjk1LxDAQhnNQcFn3JwgBz61J06btsRQ_FhZcZO_LNJnWrG0KSSr03xtQwYMzh4GH5x1eQu44SwteZA-4OPAG7GVCnWaMpzyT_IpsuGBVUpUivyE77y8sTl4LkckNcUeHA3QwGkubENAuENDTFpyDAdGCTfZWLwo1bdQSkO5tP8I0QTCzpTH0BsHTbo383XQmGDvQo5vNrzW7lbZrmD-MRXrATxz9LbnuYfS4-7lbcnp6PLUvyeH1ed82h2SQZZ1IXkglqr4CBrzvOsEAmc4V46JkpeCy7mSma805F6LMq0LmlRBcQ6RZH60tuf9-O8CI51hoDg7UZLw6N1HOZSFkHa30Hyuuxsmo2WJvIv8T-AKpG24u</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels</title><source>PubMed Central</source><source>Directory of Open Access Journals</source><source>Alma/SFX Local Collection</source><creator>Kilic, Fatma Sultan ; Kaygisiz, Bilgin ; Aydin, Sule ; Yildirim, Cafer ; Karimkhani, Hadi ; Oner, Setenay</creator><creatorcontrib>Kilic, Fatma Sultan ; Kaygisiz, Bilgin ; Aydin, Sule ; Yildirim, Cafer ; Karimkhani, Hadi ; Oner, Setenay</creatorcontrib><description>Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor necrosis factor-[alpha] (TNF-[alpha]) and interleukine-1 [beta] (IL-1[beta]) acting as acute phase cytokines in inflammation, and anti-inflammatory cytokine IL-10, in rats.Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control group were injected once intraperitoneally prior to administration of 100 [micro]l of 1% CAR into the right hind paw of the rats. The paw thickness was measured using gauge calipers (Vernier calipers) before (0 hour) and every hour afterwards for 6 hours following the inflammation induction. The cytokine levels in the blood serum obtained intracardiacally were determined after 6 hours using the enzyme-linked immunosorbent assay method. p<0.05 was considered statistically significant.Results: There was no significant difference between the 0 and 6th hour considering the paw thickness in all groups, except in the CAR group. CAR significantly increased the paw thickness at 6 hours compared to the 0 hour. All doses of PGB and INDO significantly reduced the paw thickness after 6 hours compared to the CAR group. The TNF-[alpha] and IL-1[beta] levels in the PGB and INDO groups were comparable to the control group, whereas in the CAR group, these levels were increased. The IL-10 level was enhanced in the PGB 50 mg/kg and INDO groups.Conclusion: It was observed that all doses of PGB exerted anti-inflammatory-like effects comparable to INDO, supported by their effects on the levels of cytokines.Keywords: Pregabalin, inflammation, anti-inflammatory effect, cytokines, rat</description><identifier>ISSN: 1308-8734</identifier><identifier>DOI: 10.5152/eurasianjmed.201.1261</identifier><language>eng</language><publisher>AVES</publisher><subject>Additives ; Anti-inflammatory agents ; Biological products ; Carrageenan ; Causes of ; Complications and side effects ; Cytokines ; Dosage and administration ; Drug therapy ; Edema ; Enzyme-linked immunosorbent assay ; Enzymes ; Epilepsy ; Health aspects ; Indomethacin ; Inflammation ; Necrosis ; Pain management ; Physiological aspects ; Pregabalin ; Tumor necrosis factor ; Tumors</subject><ispartof>The Eurasian journal of medicine, 2018-10, Vol.50 (3), p.156</ispartof><rights>COPYRIGHT 2018 AVES</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,865,27929,27930</link.rule.ids></links><search><creatorcontrib>Kilic, Fatma Sultan</creatorcontrib><creatorcontrib>Kaygisiz, Bilgin</creatorcontrib><creatorcontrib>Aydin, Sule</creatorcontrib><creatorcontrib>Yildirim, Cafer</creatorcontrib><creatorcontrib>Karimkhani, Hadi</creatorcontrib><creatorcontrib>Oner, Setenay</creatorcontrib><title>Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels</title><title>The Eurasian journal of medicine</title><description>Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor necrosis factor-[alpha] (TNF-[alpha]) and interleukine-1 [beta] (IL-1[beta]) acting as acute phase cytokines in inflammation, and anti-inflammatory cytokine IL-10, in rats.Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control group were injected once intraperitoneally prior to administration of 100 [micro]l of 1% CAR into the right hind paw of the rats. The paw thickness was measured using gauge calipers (Vernier calipers) before (0 hour) and every hour afterwards for 6 hours following the inflammation induction. The cytokine levels in the blood serum obtained intracardiacally were determined after 6 hours using the enzyme-linked immunosorbent assay method. p<0.05 was considered statistically significant.Results: There was no significant difference between the 0 and 6th hour considering the paw thickness in all groups, except in the CAR group. CAR significantly increased the paw thickness at 6 hours compared to the 0 hour. All doses of PGB and INDO significantly reduced the paw thickness after 6 hours compared to the CAR group. The TNF-[alpha] and IL-1[beta] levels in the PGB and INDO groups were comparable to the control group, whereas in the CAR group, these levels were increased. The IL-10 level was enhanced in the PGB 50 mg/kg and INDO groups.Conclusion: It was observed that all doses of PGB exerted anti-inflammatory-like effects comparable to INDO, supported by their effects on the levels of cytokines.Keywords: Pregabalin, inflammation, anti-inflammatory effect, cytokines, rat</description><subject>Additives</subject><subject>Anti-inflammatory agents</subject><subject>Biological products</subject><subject>Carrageenan</subject><subject>Causes of</subject><subject>Complications and side effects</subject><subject>Cytokines</subject><subject>Dosage and administration</subject><subject>Drug therapy</subject><subject>Edema</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzymes</subject><subject>Epilepsy</subject><subject>Health aspects</subject><subject>Indomethacin</subject><subject>Inflammation</subject><subject>Necrosis</subject><subject>Pain management</subject><subject>Physiological aspects</subject><subject>Pregabalin</subject><subject>Tumor necrosis factor</subject><subject>Tumors</subject><issn>1308-8734</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptjk1LxDAQhnNQcFn3JwgBz61J06btsRQ_FhZcZO_LNJnWrG0KSSr03xtQwYMzh4GH5x1eQu44SwteZA-4OPAG7GVCnWaMpzyT_IpsuGBVUpUivyE77y8sTl4LkckNcUeHA3QwGkubENAuENDTFpyDAdGCTfZWLwo1bdQSkO5tP8I0QTCzpTH0BsHTbo383XQmGDvQo5vNrzW7lbZrmD-MRXrATxz9LbnuYfS4-7lbcnp6PLUvyeH1ed82h2SQZZ1IXkglqr4CBrzvOsEAmc4V46JkpeCy7mSma805F6LMq0LmlRBcQ6RZH60tuf9-O8CI51hoDg7UZLw6N1HOZSFkHa30Hyuuxsmo2WJvIv8T-AKpG24u</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Kilic, Fatma Sultan</creator><creator>Kaygisiz, Bilgin</creator><creator>Aydin, Sule</creator><creator>Yildirim, Cafer</creator><creator>Karimkhani, Hadi</creator><creator>Oner, Setenay</creator><general>AVES</general><scope/></search><sort><creationdate>20181001</creationdate><title>Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels</title><author>Kilic, Fatma Sultan ; Kaygisiz, Bilgin ; Aydin, Sule ; Yildirim, Cafer ; Karimkhani, Hadi ; Oner, Setenay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g679-6156c38f8a0a1fbb30ae0d4c0137073169b62d9d111337485648331da62d2f013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Additives</topic><topic>Anti-inflammatory agents</topic><topic>Biological products</topic><topic>Carrageenan</topic><topic>Causes of</topic><topic>Complications and side effects</topic><topic>Cytokines</topic><topic>Dosage and administration</topic><topic>Drug therapy</topic><topic>Edema</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzymes</topic><topic>Epilepsy</topic><topic>Health aspects</topic><topic>Indomethacin</topic><topic>Inflammation</topic><topic>Necrosis</topic><topic>Pain management</topic><topic>Physiological aspects</topic><topic>Pregabalin</topic><topic>Tumor necrosis factor</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kilic, Fatma Sultan</creatorcontrib><creatorcontrib>Kaygisiz, Bilgin</creatorcontrib><creatorcontrib>Aydin, Sule</creatorcontrib><creatorcontrib>Yildirim, Cafer</creatorcontrib><creatorcontrib>Karimkhani, Hadi</creatorcontrib><creatorcontrib>Oner, Setenay</creatorcontrib><jtitle>The Eurasian journal of medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kilic, Fatma Sultan</au><au>Kaygisiz, Bilgin</au><au>Aydin, Sule</au><au>Yildirim, Cafer</au><au>Karimkhani, Hadi</au><au>Oner, Setenay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels</atitle><jtitle>The Eurasian journal of medicine</jtitle><date>2018-10-01</date><risdate>2018</risdate><volume>50</volume><issue>3</issue><spage>156</spage><pages>156-</pages><issn>1308-8734</issn><abstract>Objective: Pregabalin (PGB) is a compound used in the treatment of epilepsy, anxiety, and neuropathic pain. Experimental data also indicate that PGB can reduce inflammatory pain. We aimed to investigate the anti-inflammatory effects of PGB on carrageenan (CAR)-induced paw edema and its effects on tumor necrosis factor-[alpha] (TNF-[alpha]) and interleukine-1 [beta] (IL-1[beta]) acting as acute phase cytokines in inflammation, and anti-inflammatory cytokine IL-10, in rats.Materials and Methods: Single doses of PGB 30, 50, and 100 mg/kg and indomethacin (INDO) 5 mg/kg in the treatment groups and saline in the control group were injected once intraperitoneally prior to administration of 100 [micro]l of 1% CAR into the right hind paw of the rats. The paw thickness was measured using gauge calipers (Vernier calipers) before (0 hour) and every hour afterwards for 6 hours following the inflammation induction. The cytokine levels in the blood serum obtained intracardiacally were determined after 6 hours using the enzyme-linked immunosorbent assay method. p<0.05 was considered statistically significant.Results: There was no significant difference between the 0 and 6th hour considering the paw thickness in all groups, except in the CAR group. CAR significantly increased the paw thickness at 6 hours compared to the 0 hour. All doses of PGB and INDO significantly reduced the paw thickness after 6 hours compared to the CAR group. The TNF-[alpha] and IL-1[beta] levels in the PGB and INDO groups were comparable to the control group, whereas in the CAR group, these levels were increased. The IL-10 level was enhanced in the PGB 50 mg/kg and INDO groups.Conclusion: It was observed that all doses of PGB exerted anti-inflammatory-like effects comparable to INDO, supported by their effects on the levels of cytokines.Keywords: Pregabalin, inflammation, anti-inflammatory effect, cytokines, rat</abstract><pub>AVES</pub><doi>10.5152/eurasianjmed.201.1261</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1308-8734 |
| ispartof | The Eurasian journal of medicine, 2018-10, Vol.50 (3), p.156 |
| issn | 1308-8734 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A564465369 |
| source | PubMed Central; Directory of Open Access Journals; Alma/SFX Local Collection |
| subjects | Additives Anti-inflammatory agents Biological products Carrageenan Causes of Complications and side effects Cytokines Dosage and administration Drug therapy Edema Enzyme-linked immunosorbent assay Enzymes Epilepsy Health aspects Indomethacin Inflammation Necrosis Pain management Physiological aspects Pregabalin Tumor necrosis factor Tumors |
| title | Pregabalin Attenuates Carrageenan-Induced Acute Inflammation in Rats by Inhibiting Proinflammatory Cytokine Levels |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T16%3A03%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pregabalin%20Attenuates%20Carrageenan-Induced%20Acute%20Inflammation%20in%20Rats%20by%20Inhibiting%20Proinflammatory%20Cytokine%20Levels&rft.jtitle=The%20Eurasian%20journal%20of%20medicine&rft.au=Kilic,%20Fatma%20Sultan&rft.date=2018-10-01&rft.volume=50&rft.issue=3&rft.spage=156&rft.pages=156-&rft.issn=1308-8734&rft_id=info:doi/10.5152/eurasianjmed.201.1261&rft_dat=%3Cgale%3EA564465369%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A564465369&rfr_iscdi=true |