lipoic acid can greatly alleviate the toxic effect of AGES on SH-SY5Y cells

The aim of the study was to explore the influence of [alpha]-lipoic acid ([alpha]-LA) on the cytotoxicity of advanced glycation end-products (AGEs) against SH-SY5Y cells. AGE-bovine serum albumin (BSA) was incubated in vitro using SH-SY5Y cells as a target model, and the control group was set. Cells were exposed to AGE-BSA, and [alpha]-LA was selectively added to the cells. Cell growth and death was determined by the MTT assay, which measures cellular metabolic rate, lactate dehydrogenase (LDH) leakage rate and cellular axonal length. Immunocytochemistry was employed to detect the expression of [beta]-amyloid (A[beta]) protein in cells, and mRNA expression of amyloid precursor protein (APP) and the receptor for AGE (RAGE) were assayed by PT-PCR. The metabolism of MTT was clearly increased, the rate of LDH leakage was significantly decreased, and axonal length was significantly increased in cells treated with [alpha]-LA (0.1 g/l) as compared to untreated cells. Furthermore, the expression levels of A[beta] protein were also decreased. In addition, [alpha]-LA (0.1 g/l) markedly inhibited the expression of RAGE mRNA, and did not influence APP mRNA expression as compared the control group. [alpha]-LA (0.1 g/l) was effective at dampening the cytotoxicity of AGE-BSA, a preliminary observation that confirms the ability of [alpha]-LA to significantly alleviate the cytotoxicity of AGEs against SH-SY5Y cells. Key words: [alpha]-lipoic acid, advanced glycation end-products; Alzheimer's disease, [beta]-amyloid protein, amyloid precursor protein