ChrA.sub.SO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction
The chromate efflux pump encoding gene chrA.sub.SO was identified on the chromosome of Shewanella oneidensis MR1. Although chrA.sub.SO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant [DELTA]chrA.sub.SO exhibits a chromate sensiti...
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| Veröffentlicht in: | PloS one 2017-11, Vol.12 (11), p.e0188516 |
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| creator | Baaziz, Hiba Gambari, Cyril Boyeldieu, Anne Ali Chaouche, Amine Alatou, Radia Méjean, Vincent Jourlin-Castelli, Cécile Fons, Michel |
| description | The chromate efflux pump encoding gene chrA.sub.SO was identified on the chromosome of Shewanella oneidensis MR1. Although chrA.sub.SO is expressed without chromate, its expression level increases when Cr(VI) is added. When deleted, the resulting mutant [DELTA]chrA.sub.SO exhibits a chromate sensitive phenotype compared to that of the wild-type strain. Interestingly, heterologous expression of chrA.sub.SO in E. coli confers resistance to high chromate concentration. Moreover, expression of chrA.sub.SO in S. oneidensis and E. coli significantly improves Cr(VI) reduction. This effect could result either from extracytoplasmic chromate reduction or from a better cell survival leading to enhanced Cr(VI) reduction. |
| doi_str_mv | 10.1371/journal.pone.0188516 |
| format | Article |
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| subjects | Chromium (Metal) Genetic aspects Physiological aspects Shewanella |
| title | ChrA.sub.SO, the chromate efflux pump of Shewanella oneidensis, improves chromate survival and reduction |
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