Dexamethasone effect on the expression of transforming growth factor-[beta]1/smads signaling pathway in benign biliary stricture fibroblasts in rodent
Objective: To investigate the effects of dexamethasone on transforming growth factor (TGF)-[beta]1/Smads signaling pathway in benign biliary stricture (BBS) fibroblasts. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from Januar...
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| Veröffentlicht in: | Journal of the College of Physicians and Surgeons--Pakistan 2017-03, Vol.27 (3), p.131 |
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| container_issue | 3 |
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| container_title | Journal of the College of Physicians and Surgeons--Pakistan |
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| creator | Li, Ke-Yue Shi, Cheng-Xian Huang, Jian-Zhao Tang, Ke-li |
| description | Objective: To investigate the effects of dexamethasone on transforming growth factor (TGF)-[beta]1/Smads signaling pathway in benign biliary stricture (BBS) fibroblasts. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from January to August 2016. Methodology: Fibroblasts derived from rabbit BBS model were cultured and identified, then treated by different concentration of dexamethasone (0.02, 0.1 and 0.5 mg/ml). Dexamethasone-treated cells and non-treated control groups were incubated respectively for 48 hours. Cell proliferation was assessed using cell counting kit-8. Relative mRNA expression of TGF-[beta]1, Smad4 and Smad7 were assessed by quantitative RT-PCR. Protein expression of TGF-[beta]1 and Smad4 were investigated by Western blotting. Results: Treatment with dexamethasone significantly inhibited the proliferation of BBS fibroblasts, significantly attenuated both the mRNA and protein expression of TGF-[beta]1 and Smad4, and significantly up-regulated the mRNA expression of Smad7 in BBS fibroblasts (all p |
| format | Article |
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Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from January to August 2016. Methodology: Fibroblasts derived from rabbit BBS model were cultured and identified, then treated by different concentration of dexamethasone (0.02, 0.1 and 0.5 mg/ml). Dexamethasone-treated cells and non-treated control groups were incubated respectively for 48 hours. Cell proliferation was assessed using cell counting kit-8. Relative mRNA expression of TGF-[beta]1, Smad4 and Smad7 were assessed by quantitative RT-PCR. Protein expression of TGF-[beta]1 and Smad4 were investigated by Western blotting. Results: Treatment with dexamethasone significantly inhibited the proliferation of BBS fibroblasts, significantly attenuated both the mRNA and protein expression of TGF-[beta]1 and Smad4, and significantly up-regulated the mRNA expression of Smad7 in BBS fibroblasts (all p<0.05, 0.1-0.5 mg/ml), and exhibited in a dose-dependent manner. Conclusion: TGF-[beta]1/Smads signaling pathway may play an important role in BBS progression; dexamethasone significantly altered the expression of TGF-[beta]1/Smads signaling pathway and significantly inhibited cell proliferation in rabbit BBS fibroblasts. Therefore, dexamethasone may be a therapeutic option for the prevention of BBS. Key Words: Benign biliary stricture. Fibroblasts. TGF-[beta]1/Smads. Dexamethasone. Rabbit.</description><identifier>ISSN: 1022-386X</identifier><language>eng</language><publisher>College of Physicians and Surgeons Pakistan</publisher><subject>Analysis ; Bone morphogenetic proteins ; Central nervous system depressants ; Dexamethasone ; Dosage and administration ; Health aspects ; Physiological aspects ; Smad proteins ; Transforming growth factors</subject><ispartof>Journal of the College of Physicians and Surgeons--Pakistan, 2017-03, Vol.27 (3), p.131</ispartof><rights>COPYRIGHT 2017 College of Physicians and Surgeons Pakistan</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Li, Ke-Yue</creatorcontrib><creatorcontrib>Shi, Cheng-Xian</creatorcontrib><creatorcontrib>Huang, Jian-Zhao</creatorcontrib><creatorcontrib>Tang, Ke-li</creatorcontrib><title>Dexamethasone effect on the expression of transforming growth factor-[beta]1/smads signaling pathway in benign biliary stricture fibroblasts in rodent</title><title>Journal of the College of Physicians and Surgeons--Pakistan</title><description>Objective: To investigate the effects of dexamethasone on transforming growth factor (TGF)-[beta]1/Smads signaling pathway in benign biliary stricture (BBS) fibroblasts. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from January to August 2016. Methodology: Fibroblasts derived from rabbit BBS model were cultured and identified, then treated by different concentration of dexamethasone (0.02, 0.1 and 0.5 mg/ml). Dexamethasone-treated cells and non-treated control groups were incubated respectively for 48 hours. Cell proliferation was assessed using cell counting kit-8. Relative mRNA expression of TGF-[beta]1, Smad4 and Smad7 were assessed by quantitative RT-PCR. Protein expression of TGF-[beta]1 and Smad4 were investigated by Western blotting. Results: Treatment with dexamethasone significantly inhibited the proliferation of BBS fibroblasts, significantly attenuated both the mRNA and protein expression of TGF-[beta]1 and Smad4, and significantly up-regulated the mRNA expression of Smad7 in BBS fibroblasts (all p<0.05, 0.1-0.5 mg/ml), and exhibited in a dose-dependent manner. Conclusion: TGF-[beta]1/Smads signaling pathway may play an important role in BBS progression; dexamethasone significantly altered the expression of TGF-[beta]1/Smads signaling pathway and significantly inhibited cell proliferation in rabbit BBS fibroblasts. Therefore, dexamethasone may be a therapeutic option for the prevention of BBS. Key Words: Benign biliary stricture. Fibroblasts. TGF-[beta]1/Smads. Dexamethasone. Rabbit.</description><subject>Analysis</subject><subject>Bone morphogenetic proteins</subject><subject>Central nervous system depressants</subject><subject>Dexamethasone</subject><subject>Dosage and administration</subject><subject>Health aspects</subject><subject>Physiological aspects</subject><subject>Smad proteins</subject><subject>Transforming growth factors</subject><issn>1022-386X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptkM9KxDAQxntQcF19h4Dnats0TXpc1n8LC172IIgsk3TSRtpkSSK7-yI-r1n04EHmMHzf_OaDmbNsVhZVlVPRvF5klyF8FAVlpRCz7OseDzBhHCA4iwS1RhWJsyQOSR12HkMwSTpNogcbtPOTsT3pvdvHgWhQ0fn8TWKE9_IuTNAFEkxvYTxRO4jDHo7EWCLRJptIMxrwRxKiNyp-eiTaSO_kCCGGE-ddhzZeZecaxoDXv32ebR4fNsvnfP3ytFou1nnfcJHXkjEsWsZLChS4wEI1vK2aClsqNe8a1pSirlkadB1Xdcla3VUgOVWC87ag8-zmJ7aHEbfGapeOVJMJaruo24rRRlCRqNt_qFQdTkalt2mT_D8L34YAdDo</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Li, Ke-Yue</creator><creator>Shi, Cheng-Xian</creator><creator>Huang, Jian-Zhao</creator><creator>Tang, Ke-li</creator><general>College of Physicians and Surgeons Pakistan</general><scope/></search><sort><creationdate>20170301</creationdate><title>Dexamethasone effect on the expression of transforming growth factor-[beta]1/smads signaling pathway in benign biliary stricture fibroblasts in rodent</title><author>Li, Ke-Yue ; Shi, Cheng-Xian ; Huang, Jian-Zhao ; Tang, Ke-li</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g678-4b55e095713a3a78e0c679262e93bf7d656184458e0dd7c4159fd2ab73c877903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Bone morphogenetic proteins</topic><topic>Central nervous system depressants</topic><topic>Dexamethasone</topic><topic>Dosage and administration</topic><topic>Health aspects</topic><topic>Physiological aspects</topic><topic>Smad proteins</topic><topic>Transforming growth factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ke-Yue</creatorcontrib><creatorcontrib>Shi, Cheng-Xian</creatorcontrib><creatorcontrib>Huang, Jian-Zhao</creatorcontrib><creatorcontrib>Tang, Ke-li</creatorcontrib><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ke-Yue</au><au>Shi, Cheng-Xian</au><au>Huang, Jian-Zhao</au><au>Tang, Ke-li</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dexamethasone effect on the expression of transforming growth factor-[beta]1/smads signaling pathway in benign biliary stricture fibroblasts in rodent</atitle><jtitle>Journal of the College of Physicians and Surgeons--Pakistan</jtitle><date>2017-03-01</date><risdate>2017</risdate><volume>27</volume><issue>3</issue><spage>131</spage><pages>131-</pages><issn>1022-386X</issn><abstract>Objective: To investigate the effects of dexamethasone on transforming growth factor (TGF)-[beta]1/Smads signaling pathway in benign biliary stricture (BBS) fibroblasts. Study Design: An experimental study. Place and Duration of Study: Guizhou Medical University, Guiyang, Guizhou, China, from January to August 2016. Methodology: Fibroblasts derived from rabbit BBS model were cultured and identified, then treated by different concentration of dexamethasone (0.02, 0.1 and 0.5 mg/ml). Dexamethasone-treated cells and non-treated control groups were incubated respectively for 48 hours. Cell proliferation was assessed using cell counting kit-8. Relative mRNA expression of TGF-[beta]1, Smad4 and Smad7 were assessed by quantitative RT-PCR. Protein expression of TGF-[beta]1 and Smad4 were investigated by Western blotting. Results: Treatment with dexamethasone significantly inhibited the proliferation of BBS fibroblasts, significantly attenuated both the mRNA and protein expression of TGF-[beta]1 and Smad4, and significantly up-regulated the mRNA expression of Smad7 in BBS fibroblasts (all p<0.05, 0.1-0.5 mg/ml), and exhibited in a dose-dependent manner. Conclusion: TGF-[beta]1/Smads signaling pathway may play an important role in BBS progression; dexamethasone significantly altered the expression of TGF-[beta]1/Smads signaling pathway and significantly inhibited cell proliferation in rabbit BBS fibroblasts. Therefore, dexamethasone may be a therapeutic option for the prevention of BBS. Key Words: Benign biliary stricture. Fibroblasts. TGF-[beta]1/Smads. Dexamethasone. Rabbit.</abstract><pub>College of Physicians and Surgeons Pakistan</pub></addata></record> |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Analysis Bone morphogenetic proteins Central nervous system depressants Dexamethasone Dosage and administration Health aspects Physiological aspects Smad proteins Transforming growth factors |
| title | Dexamethasone effect on the expression of transforming growth factor-[beta]1/smads signaling pathway in benign biliary stricture fibroblasts in rodent |
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