The effect of spectral light quality on in vitro culture of sugarcane/ Efeito da qualidade espectral da luz no cultivo in vitro de cana-de-acucar
The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 [micro]M 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (7...
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description | The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 [micro]M 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis. Keywords: in vitro propagation, light wavelength, Saccharum spp. Foram investigados os efeitos de diferentes tratamentos de qualidade da luz sobre o crescimento e multiplicacao in vitro de cana-de-acucar (RB867515). As plantas foram cultivadas em meio MS contendo 1,3 [micro]M de 6-benzilaminopurina (BAP) e expostas a cinco tratamentos de luz: tres combinacoes de LED azul/vermelho (70:30, 50:50, 30:70), LED branco e lampadas fluorescentes, durante 24 dias. Entre os tratamentos de luz de LED, a combinacao azul/vermelho na proporcao 50:50 apresentou os melhores resultados para altura, massa fresca, numero de folhas e multiplicacao de brotos. Maior teor de pigmentos fotossinteticos tambem foi obtido com a utilizacao de LEDs. Os resultados sugerem que a qualidade da luz emitida pelos LEDs foi adequada para o crescimento e desenvolvimento das plantas e pode ser utilizada como fonte de luz alternativa e economica para a micropropagacao da variedade de cana-de-acucar estudada. Palavras-chave: propagacao in vitro, comprimento de onda da luz, Saccharum spp. |
doi_str_mv | 10.4025/actascibiolsci.v38i2.31109 |
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The plantlets were cultivated on MS medium containing 1.3 [micro]M 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis. Keywords: in vitro propagation, light wavelength, Saccharum spp. Foram investigados os efeitos de diferentes tratamentos de qualidade da luz sobre o crescimento e multiplicacao in vitro de cana-de-acucar (RB867515). As plantas foram cultivadas em meio MS contendo 1,3 [micro]M de 6-benzilaminopurina (BAP) e expostas a cinco tratamentos de luz: tres combinacoes de LED azul/vermelho (70:30, 50:50, 30:70), LED branco e lampadas fluorescentes, durante 24 dias. Entre os tratamentos de luz de LED, a combinacao azul/vermelho na proporcao 50:50 apresentou os melhores resultados para altura, massa fresca, numero de folhas e multiplicacao de brotos. Maior teor de pigmentos fotossinteticos tambem foi obtido com a utilizacao de LEDs. Os resultados sugerem que a qualidade da luz emitida pelos LEDs foi adequada para o crescimento e desenvolvimento das plantas e pode ser utilizada como fonte de luz alternativa e economica para a micropropagacao da variedade de cana-de-acucar estudada. Palavras-chave: propagacao in vitro, comprimento de onda da luz, Saccharum spp.</description><identifier>ISSN: 1679-9283</identifier><identifier>DOI: 10.4025/actascibiolsci.v38i2.31109</identifier><language>spa</language><publisher>Universidade Estadual de Maringa</publisher><ispartof>Acta scientiarum. 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Biological sciences</title><description>The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 [micro]M 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis. Keywords: in vitro propagation, light wavelength, Saccharum spp. Foram investigados os efeitos de diferentes tratamentos de qualidade da luz sobre o crescimento e multiplicacao in vitro de cana-de-acucar (RB867515). As plantas foram cultivadas em meio MS contendo 1,3 [micro]M de 6-benzilaminopurina (BAP) e expostas a cinco tratamentos de luz: tres combinacoes de LED azul/vermelho (70:30, 50:50, 30:70), LED branco e lampadas fluorescentes, durante 24 dias. Entre os tratamentos de luz de LED, a combinacao azul/vermelho na proporcao 50:50 apresentou os melhores resultados para altura, massa fresca, numero de folhas e multiplicacao de brotos. Maior teor de pigmentos fotossinteticos tambem foi obtido com a utilizacao de LEDs. Os resultados sugerem que a qualidade da luz emitida pelos LEDs foi adequada para o crescimento e desenvolvimento das plantas e pode ser utilizada como fonte de luz alternativa e economica para a micropropagacao da variedade de cana-de-acucar estudada. 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Biological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Araujo Silva, Marina Medeiros</au><au>de Oliveira, Arquimedes Liberal Barboza</au><au>Oliveira-Filho, Ronaldo Alves</au><au>Camara, Terezinha</au><au>Willadino, Lilia</au><au>Gouveia-Neto, Artur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of spectral light quality on in vitro culture of sugarcane/ Efeito da qualidade espectral da luz no cultivo in vitro de cana-de-acucar</atitle><jtitle>Acta scientiarum. Biological sciences</jtitle><date>2016-04-01</date><risdate>2016</risdate><volume>38</volume><issue>2</issue><spage>157</spage><pages>157-</pages><issn>1679-9283</issn><abstract>The effects of different light quality treatments on in vitro growth and multiplication of sugarcane (RB867515) were investigated. The plantlets were cultivated on MS medium containing 1.3 [micro]M 6-benzylaminopurine (BAP), and exposed to five light treatments: three combinations of blue/red LED (70:30, 50:50, 30:70), white-LED and fluorescent lamps, during 24 days. Among the LED light treatments, blue/red combination in 50:50 proportions proved to have the best results for stem length, fresh mass, leaf number and shoot multiplication. Higher content of photosynthetic pigments was also obtained with LEDs. Results suggested that the light quality emitted by LEDs was suitable for plant growth and development and it may be used as alternative and economic light source for micropropagation of sugarcane variety under analysis. Keywords: in vitro propagation, light wavelength, Saccharum spp. Foram investigados os efeitos de diferentes tratamentos de qualidade da luz sobre o crescimento e multiplicacao in vitro de cana-de-acucar (RB867515). As plantas foram cultivadas em meio MS contendo 1,3 [micro]M de 6-benzilaminopurina (BAP) e expostas a cinco tratamentos de luz: tres combinacoes de LED azul/vermelho (70:30, 50:50, 30:70), LED branco e lampadas fluorescentes, durante 24 dias. Entre os tratamentos de luz de LED, a combinacao azul/vermelho na proporcao 50:50 apresentou os melhores resultados para altura, massa fresca, numero de folhas e multiplicacao de brotos. Maior teor de pigmentos fotossinteticos tambem foi obtido com a utilizacao de LEDs. Os resultados sugerem que a qualidade da luz emitida pelos LEDs foi adequada para o crescimento e desenvolvimento das plantas e pode ser utilizada como fonte de luz alternativa e economica para a micropropagacao da variedade de cana-de-acucar estudada. Palavras-chave: propagacao in vitro, comprimento de onda da luz, Saccharum spp.</abstract><pub>Universidade Estadual de Maringa</pub><doi>10.4025/actascibiolsci.v38i2.31109</doi></addata></record> |
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title | The effect of spectral light quality on in vitro culture of sugarcane/ Efeito da qualidade espectral da luz no cultivo in vitro de cana-de-acucar |
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