Stable physically adsorbed coating of poly-vinyl alcohol for the separation of basic proteins
In aqueous capillary electrophoresis, the electroosmotic flow can be strongly suppressed by coating the inner surface of the capillary. In the present work hydrophilic coating of 4% polyvinyl alcohol ( PVA ) has been used for the analysis of basic proteins. The coating is simple and easy to obtain....
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| Veröffentlicht in: | Journal of analytical chemistry (New York, N.Y.) N.Y.), 2012-03, Vol.67 (3), p.278-283 |
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| container_title | Journal of analytical chemistry (New York, N.Y.) |
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| creator | Baderia, Vishal Kumar Gowri, V. Sorna Sanghi, Sunil Kumar Shukla, Ankita Singh, Deepesh Kumar Sanghi, Shail Bala |
| description | In aqueous capillary electrophoresis, the electroosmotic flow can be strongly suppressed by coating the inner surface of the capillary. In the present work hydrophilic coating of 4% polyvinyl alcohol (
PVA
) has been used for the analysis of basic proteins. The coating is simple and easy to obtain. The separation of ribonuclease and α-chymotrypsin has been uniquely done with other three basic proteins (lysozyme, cytochrome-c and trypsin) using a buffer 11.60 mM sodium acetate and 18.40 mM acetic acid at pH 4.5 in addition to positive power supply of 20 kV at 25°C. Detection was performed using UV detector at 230 nm. The proposed PVA coated capillary provides reproducible separation of five basic proteins within 10 min with RSD values for mobility bellow 1.4% (
n
= 6) for all the five basic proteins. The stability of coated capillary has been checked up to 40 runs. The viscosity measurement for 4% PVA have been studied and scanning electron microscope (
SEM
) images obtained to make it compatible with future micro-chip applications. |
| doi_str_mv | 10.1134/S1061934812030112 |
| format | Article |
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PVA
) has been used for the analysis of basic proteins. The coating is simple and easy to obtain. The separation of ribonuclease and α-chymotrypsin has been uniquely done with other three basic proteins (lysozyme, cytochrome-c and trypsin) using a buffer 11.60 mM sodium acetate and 18.40 mM acetic acid at pH 4.5 in addition to positive power supply of 20 kV at 25°C. Detection was performed using UV detector at 230 nm. The proposed PVA coated capillary provides reproducible separation of five basic proteins within 10 min with RSD values for mobility bellow 1.4% (
n
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SEM
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PVA
) has been used for the analysis of basic proteins. The coating is simple and easy to obtain. The separation of ribonuclease and α-chymotrypsin has been uniquely done with other three basic proteins (lysozyme, cytochrome-c and trypsin) using a buffer 11.60 mM sodium acetate and 18.40 mM acetic acid at pH 4.5 in addition to positive power supply of 20 kV at 25°C. Detection was performed using UV detector at 230 nm. The proposed PVA coated capillary provides reproducible separation of five basic proteins within 10 min with RSD values for mobility bellow 1.4% (
n
= 6) for all the five basic proteins. The stability of coated capillary has been checked up to 40 runs. The viscosity measurement for 4% PVA have been studied and scanning electron microscope (
SEM
) images obtained to make it compatible with future micro-chip applications.</description><subject>Acetic acid</subject><subject>Analysis</subject><subject>Analytical Chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Detectors</subject><subject>Enzymes</subject><subject>Hydrolases</subject><subject>Organic acids</subject><subject>Ribonuclease</subject><issn>1061-9348</issn><issn>1608-3199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNp90U1LxDAQBuAiCq4fP8BbwJOHrkmbtulRxC8QBFePUtLJtJslm5Skiv33Zlkvi4vkkJB53jnMJMkFo3PGcn69YLRkdc4Fy2hOGcsOkhkrqUhzVteH8R3L6aZ-nJyEsKKU1oKVs-RjMcrWIBmWU9AgjZmIVMH5FhUBJ0dte-I6MjgzpV_aToZIA27pDOmcJ-MSScBB-gid3cBWxjZk8G5EbcNZctRJE_D89z5N3u_v3m4f0-eXh6fbm-cUeFaNKVRUVRwABRMFcs4zKMpMAuUltKotEDuUSuVFUWcVqBxKUVDeCiYrAVTx_DS53PbtpcFG286NXsJaB2hueFXTqhRVHlW6R_Vo0UvjLHY6fu_4-R4fj8K1hr2Bq51ANCN-j738DKF5WrzuWra14F0IHrtm8Hot_dQw2mxW2vxZacxk20yI1vbom5X79DZO9p_QD1zQoaw</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Baderia, Vishal Kumar</creator><creator>Gowri, V. Sorna</creator><creator>Sanghi, Sunil Kumar</creator><creator>Shukla, Ankita</creator><creator>Singh, Deepesh Kumar</creator><creator>Sanghi, Shail Bala</creator><general>SP MAIK Nauka/Interperiodica</general><general>Springer</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope></search><sort><creationdate>20120301</creationdate><title>Stable physically adsorbed coating of poly-vinyl alcohol for the separation of basic proteins</title><author>Baderia, Vishal Kumar ; Gowri, V. Sorna ; Sanghi, Sunil Kumar ; Shukla, Ankita ; Singh, Deepesh Kumar ; Sanghi, Shail Bala</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-c70d74cce8185e4442c562ac046cbdb5eefeadd355927cd3c68504b81a78c0d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Acetic acid</topic><topic>Analysis</topic><topic>Analytical Chemistry</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Detectors</topic><topic>Enzymes</topic><topic>Hydrolases</topic><topic>Organic acids</topic><topic>Ribonuclease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baderia, Vishal Kumar</creatorcontrib><creatorcontrib>Gowri, V. Sorna</creatorcontrib><creatorcontrib>Sanghi, Sunil Kumar</creatorcontrib><creatorcontrib>Shukla, Ankita</creatorcontrib><creatorcontrib>Singh, Deepesh Kumar</creatorcontrib><creatorcontrib>Sanghi, Shail Bala</creatorcontrib><collection>CrossRef</collection><collection>Gale In Context: Science</collection><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baderia, Vishal Kumar</au><au>Gowri, V. Sorna</au><au>Sanghi, Sunil Kumar</au><au>Shukla, Ankita</au><au>Singh, Deepesh Kumar</au><au>Sanghi, Shail Bala</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable physically adsorbed coating of poly-vinyl alcohol for the separation of basic proteins</atitle><jtitle>Journal of analytical chemistry (New York, N.Y.)</jtitle><stitle>J Anal Chem</stitle><date>2012-03-01</date><risdate>2012</risdate><volume>67</volume><issue>3</issue><spage>278</spage><epage>283</epage><pages>278-283</pages><issn>1061-9348</issn><eissn>1608-3199</eissn><abstract>In aqueous capillary electrophoresis, the electroosmotic flow can be strongly suppressed by coating the inner surface of the capillary. In the present work hydrophilic coating of 4% polyvinyl alcohol (
PVA
) has been used for the analysis of basic proteins. The coating is simple and easy to obtain. The separation of ribonuclease and α-chymotrypsin has been uniquely done with other three basic proteins (lysozyme, cytochrome-c and trypsin) using a buffer 11.60 mM sodium acetate and 18.40 mM acetic acid at pH 4.5 in addition to positive power supply of 20 kV at 25°C. Detection was performed using UV detector at 230 nm. The proposed PVA coated capillary provides reproducible separation of five basic proteins within 10 min with RSD values for mobility bellow 1.4% (
n
= 6) for all the five basic proteins. The stability of coated capillary has been checked up to 40 runs. The viscosity measurement for 4% PVA have been studied and scanning electron microscope (
SEM
) images obtained to make it compatible with future micro-chip applications.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S1061934812030112</doi><tpages>6</tpages></addata></record> |
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| issn | 1061-9348 1608-3199 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A479076873 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Acetic acid Analysis Analytical Chemistry Chemistry Chemistry and Materials Science Detectors Enzymes Hydrolases Organic acids Ribonuclease |
| title | Stable physically adsorbed coating of poly-vinyl alcohol for the separation of basic proteins |
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