Dissociation of Increases in PGC-1[alpha] and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise

Dissociation of Increases in PGC-1[alpha] and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise

Muscle activation as well as changes in peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1[alpha]) following high-intensity interval exercise (HIIE) were examined in young healthy men (n = 8; age, 21.9±2.2 yrs; VO.sub.2 peak, 53.1±6.4 ml/min/kg; peak work rate, 317±23.5 watt...

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Veröffentlicht in:PloS one 2013-08, Vol.8 (8), p.e71623
Hauptverfasser: Edgett, Brittany A, Foster, William S, Hankinson, Paul B, Simpson, Craig A, Little, Jonathan P, Graham, Ryan B, Gurd, Brendon J
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container_title PloS one
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creator Edgett, Brittany A
Foster, William S
Hankinson, Paul B
Simpson, Craig A
Little, Jonathan P
Graham, Ryan B
Gurd, Brendon J
description Muscle activation as well as changes in peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1[alpha]) following high-intensity interval exercise (HIIE) were examined in young healthy men (n = 8; age, 21.9±2.2 yrs; VO.sub.2 peak, 53.1±6.4 ml/min/kg; peak work rate, 317±23.5 watts). On each of 3 visits HIIE was performed on a cycle ergometer at a target intensity of 73, 100, or 133% of peak work rate. Muscle biopsies were taken at rest and three hours after each exercise condition. Total work was not different between conditions (~730 kJ) while average power output (73%, 237±21; 100%, 323±26; 133%, 384±35 watts) and EMG derived muscle activation (73%, 1262±605; 100%, 2089±737; 133%, 3029±1206 total integrated EMG per interval) increased in an intensity dependent fashion. PGC-1[alpha] mRNA was elevated after all three conditions (p
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On each of 3 visits HIIE was performed on a cycle ergometer at a target intensity of 73, 100, or 133% of peak work rate. Muscle biopsies were taken at rest and three hours after each exercise condition. Total work was not different between conditions (~730 kJ) while average power output (73%, 237±21; 100%, 323±26; 133%, 384±35 watts) and EMG derived muscle activation (73%, 1262±605; 100%, 2089±737; 133%, 3029±1206 total integrated EMG per interval) increased in an intensity dependent fashion. PGC-1[alpha] mRNA was elevated after all three conditions (p&lt;0.05), with a greater increase observed following the 100% condition (~9 fold, p&lt;0.05) compared to both the 73 and 133% conditions (~4 fold). When expressed relative to muscle activation, the increase in PGC-1[alpha] mRNA for the 133% condition was less than that for the 73 and 100% conditions (p&lt;0.05). SIRT1 mRNA was also elevated after all three conditions (~1.4 fold, p&lt;0.05), with no difference between conditions. These findings suggest that intensity-dependent increases in PGC-1[alpha] mRNA following submaximal exercise are largely due to increases in muscle recruitment. As well, the blunted response of PGC-1[alpha] mRNA expression following supramaximal exercise may indicate that signalling mediated activation of PGC-1[alpha] may also be blunted. 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On each of 3 visits HIIE was performed on a cycle ergometer at a target intensity of 73, 100, or 133% of peak work rate. Muscle biopsies were taken at rest and three hours after each exercise condition. Total work was not different between conditions (~730 kJ) while average power output (73%, 237±21; 100%, 323±26; 133%, 384±35 watts) and EMG derived muscle activation (73%, 1262±605; 100%, 2089±737; 133%, 3029±1206 total integrated EMG per interval) increased in an intensity dependent fashion. PGC-1[alpha] mRNA was elevated after all three conditions (p&lt;0.05), with a greater increase observed following the 100% condition (~9 fold, p&lt;0.05) compared to both the 73 and 133% conditions (~4 fold). When expressed relative to muscle activation, the increase in PGC-1[alpha] mRNA for the 133% condition was less than that for the 73 and 100% conditions (p&lt;0.05). SIRT1 mRNA was also elevated after all three conditions (~1.4 fold, p&lt;0.05), with no difference between conditions. 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title Dissociation of Increases in PGC-1[alpha] and Its Regulators from Exercise Intensity and Muscle Activation Following Acute Exercise
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