Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro
Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote l...
Gespeichert in:
Veröffentlicht in: | PloS one 2012-06, Vol.7 (6) |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | |
---|---|
container_issue | 6 |
container_start_page | |
container_title | PloS one |
container_volume | 7 |
creator | Yeh, Jonathan R Zhang, Xiangfan Nagano, Makoto C |
description | Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote long-term SSC self-renewal. Using a SSC culture system, we have recently reported that Wnt5a promotes SSC self-renewal through a [beta]-catenin-independent Wnt mechanism whereas the [beta]-catenin-dependent Wnt pathway is not active in SSCs. In contrast, another study has reported that Wnt3a promotes SSC self-renewal through the [beta]-catenin-dependent pathway, as it can stimulate the proliferation of a spermatogonia cell line. To reconcile these two contradictory reports, we assessed Wnt3a effects on SSCs and progenitor cells, rather than a cell line, in vitro. We observed that Wnt3a induced [beta]-catenin-dependent signalling in a large subset of germ cells and increased SSC numbers. However, further investigation revealed that cell populations with greater [beta]-catenin-signalling activity contained fewer SSCs. The increased maintenance of SSCs by Wnt3a coincided with more active cell cycling and the formation of germ cell aggregates, or communities, under feeder-free conditions. Therefore, the results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities, which indirectly support SSCs and act as an in vitro niche. |
doi_str_mv | 10.1371/journal.pone.0040002 |
format | Article |
fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A477008675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A477008675</galeid><sourcerecordid>A477008675</sourcerecordid><originalsourceid>FETCH-LOGICAL-g1665-cdf8061f9c1d1fe52b0f91bfcb1fbc1ddab05bc5522d0db9c86227443660c3fe3</originalsourceid><addsrcrecordid>eNqNkF9LwzAUxYsoOKffwIeAIPjQLmnatH0cxT-FycDqfBAZSZp0GWlSmhT8-Bb0YQMf5D6cy-F37oEbBNcIRghnaLG342CojnprRARhAiGMT4IZKnAckhji04P9PLhwbg9hinNCZkFTmUYNgntwL-UkDlgJ3o3HdPHBhKefYUm9MMqAWrVTh1amBfXY93bw4NmOToC6F0NHvW2tUVSD2osOlEJrByoDNsoP9jI4k1Q7cfWr8-Dt4f61fApX68eqXK7CFhGShryROSRIFhw1SIo0ZlAWiEnOkGST11AGU8bTNI4b2LCC5ySOsyTBhECOpcDz4Obnbku12CojrR8o75Tj22WSZRDmJEsnKvqDmqYRneLTC6Wa_KPA3VFgYrz48i0dndtW9cv_2fXmmL09YHeCar9zVo9eWeMOwW_0uZOJ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Yeh, Jonathan R ; Zhang, Xiangfan ; Nagano, Makoto C</creator><creatorcontrib>Yeh, Jonathan R ; Zhang, Xiangfan ; Nagano, Makoto C</creatorcontrib><description>Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote long-term SSC self-renewal. Using a SSC culture system, we have recently reported that Wnt5a promotes SSC self-renewal through a [beta]-catenin-independent Wnt mechanism whereas the [beta]-catenin-dependent Wnt pathway is not active in SSCs. In contrast, another study has reported that Wnt3a promotes SSC self-renewal through the [beta]-catenin-dependent pathway, as it can stimulate the proliferation of a spermatogonia cell line. To reconcile these two contradictory reports, we assessed Wnt3a effects on SSCs and progenitor cells, rather than a cell line, in vitro. We observed that Wnt3a induced [beta]-catenin-dependent signalling in a large subset of germ cells and increased SSC numbers. However, further investigation revealed that cell populations with greater [beta]-catenin-signalling activity contained fewer SSCs. The increased maintenance of SSCs by Wnt3a coincided with more active cell cycling and the formation of germ cell aggregates, or communities, under feeder-free conditions. Therefore, the results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities, which indirectly support SSCs and act as an in vitro niche.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0040002</identifier><language>eng</language><publisher>Public Library of Science</publisher><subject>Analysis ; Fibroblast growth factors ; Stem cells</subject><ispartof>PloS one, 2012-06, Vol.7 (6)</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Yeh, Jonathan R</creatorcontrib><creatorcontrib>Zhang, Xiangfan</creatorcontrib><creatorcontrib>Nagano, Makoto C</creatorcontrib><title>Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro</title><title>PloS one</title><description>Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote long-term SSC self-renewal. Using a SSC culture system, we have recently reported that Wnt5a promotes SSC self-renewal through a [beta]-catenin-independent Wnt mechanism whereas the [beta]-catenin-dependent Wnt pathway is not active in SSCs. In contrast, another study has reported that Wnt3a promotes SSC self-renewal through the [beta]-catenin-dependent pathway, as it can stimulate the proliferation of a spermatogonia cell line. To reconcile these two contradictory reports, we assessed Wnt3a effects on SSCs and progenitor cells, rather than a cell line, in vitro. We observed that Wnt3a induced [beta]-catenin-dependent signalling in a large subset of germ cells and increased SSC numbers. However, further investigation revealed that cell populations with greater [beta]-catenin-signalling activity contained fewer SSCs. The increased maintenance of SSCs by Wnt3a coincided with more active cell cycling and the formation of germ cell aggregates, or communities, under feeder-free conditions. Therefore, the results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities, which indirectly support SSCs and act as an in vitro niche.</description><subject>Analysis</subject><subject>Fibroblast growth factors</subject><subject>Stem cells</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNkF9LwzAUxYsoOKffwIeAIPjQLmnatH0cxT-FycDqfBAZSZp0GWlSmhT8-Bb0YQMf5D6cy-F37oEbBNcIRghnaLG342CojnprRARhAiGMT4IZKnAckhji04P9PLhwbg9hinNCZkFTmUYNgntwL-UkDlgJ3o3HdPHBhKefYUm9MMqAWrVTh1amBfXY93bw4NmOToC6F0NHvW2tUVSD2osOlEJrByoDNsoP9jI4k1Q7cfWr8-Dt4f61fApX68eqXK7CFhGShryROSRIFhw1SIo0ZlAWiEnOkGST11AGU8bTNI4b2LCC5ySOsyTBhECOpcDz4Obnbku12CojrR8o75Tj22WSZRDmJEsnKvqDmqYRneLTC6Wa_KPA3VFgYrz48i0dndtW9cv_2fXmmL09YHeCar9zVo9eWeMOwW_0uZOJ</recordid><startdate>20120628</startdate><enddate>20120628</enddate><creator>Yeh, Jonathan R</creator><creator>Zhang, Xiangfan</creator><creator>Nagano, Makoto C</creator><general>Public Library of Science</general><scope>IOV</scope><scope>ISR</scope></search><sort><creationdate>20120628</creationdate><title>Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro</title><author>Yeh, Jonathan R ; Zhang, Xiangfan ; Nagano, Makoto C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g1665-cdf8061f9c1d1fe52b0f91bfcb1fbc1ddab05bc5522d0db9c86227443660c3fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analysis</topic><topic>Fibroblast growth factors</topic><topic>Stem cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yeh, Jonathan R</creatorcontrib><creatorcontrib>Zhang, Xiangfan</creatorcontrib><creatorcontrib>Nagano, Makoto C</creatorcontrib><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yeh, Jonathan R</au><au>Zhang, Xiangfan</au><au>Nagano, Makoto C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro</atitle><jtitle>PloS one</jtitle><date>2012-06-28</date><risdate>2012</risdate><volume>7</volume><issue>6</issue><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Proper regulation of spermatogonial stem cells (SSCs) is crucial for sustaining steady-state spermatogenesis. Previous work has identified several paracrine factors involved in this regulation, in particular, glial cell line-derived neurotrophic factor and fibroblast growth factor 2, which promote long-term SSC self-renewal. Using a SSC culture system, we have recently reported that Wnt5a promotes SSC self-renewal through a [beta]-catenin-independent Wnt mechanism whereas the [beta]-catenin-dependent Wnt pathway is not active in SSCs. In contrast, another study has reported that Wnt3a promotes SSC self-renewal through the [beta]-catenin-dependent pathway, as it can stimulate the proliferation of a spermatogonia cell line. To reconcile these two contradictory reports, we assessed Wnt3a effects on SSCs and progenitor cells, rather than a cell line, in vitro. We observed that Wnt3a induced [beta]-catenin-dependent signalling in a large subset of germ cells and increased SSC numbers. However, further investigation revealed that cell populations with greater [beta]-catenin-signalling activity contained fewer SSCs. The increased maintenance of SSCs by Wnt3a coincided with more active cell cycling and the formation of germ cell aggregates, or communities, under feeder-free conditions. Therefore, the results of this study suggest that Wnt3a selectively stimulates proliferation of progenitors that are committed to differentiation or are in the process of exiting the SSC state, leading to enhanced formation of germ cell communities, which indirectly support SSCs and act as an in vitro niche.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0040002</doi></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2012-06, Vol.7 (6) |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_gale_infotracmisc_A477008675 |
source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
subjects | Analysis Fibroblast growth factors Stem cells |
title | Indirect Effects of Wnt3a/[beta]-Catenin Signalling Support Mouse Spermatogonial Stem Cells In Vitro |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T02%3A51%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Indirect%20Effects%20of%20Wnt3a/%5Bbeta%5D-Catenin%20Signalling%20Support%20Mouse%20Spermatogonial%20Stem%20Cells%20In%20Vitro&rft.jtitle=PloS%20one&rft.au=Yeh,%20Jonathan%20R&rft.date=2012-06-28&rft.volume=7&rft.issue=6&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0040002&rft_dat=%3Cgale%3EA477008675%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A477008675&rfr_iscdi=true |