Correlation between chronic inflamation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum/Correlacao entre inflamacao cronica, imunomarcacao e carga parasitaria no sistema genital de cadelas naturalmente infectadas com Leishmania infantum

The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum. Animals (n = 10) used in this study were from the Department of Vector Control and...

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Hauptverfasser: Gomes de Oliveira, Vinicius Vasconcelos, Barros de Macedo, Simone Regina, de Araujo, Flabio Ribeiro, do Nascimento Ramos, Carlos Alberto, Nascimento Ramos, Rafael Antonio, Lyra Maia, Frederico Celso, Alves, Leucio Camara, da Silva, Jr., Valdemiro Amaro
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creator Gomes de Oliveira, Vinicius Vasconcelos
Barros de Macedo, Simone Regina
de Araujo, Flabio Ribeiro
do Nascimento Ramos, Carlos Alberto
Nascimento Ramos, Rafael Antonio
Lyra Maia, Frederico Celso
Alves, Leucio Camara
da Silva, Jr., Valdemiro Amaro
description The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum. Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated. Key words: canine visceral leishmaniasis, genital pathology, immunohistochemistry, qPCR. Considerando a falta de estudos sobre lesoes nos orgaos genitais de cadelas naturalmente infectadas por Leishmania infantum, o objetivo do presente estudo foi investigar a correlacao entre reacao inflamatoria crocanica, imunomarcacao e carga parasitaria, no sistema genital. Dez animais foram fornecidos pelo Departamento de Controle de Vetores e Vigilancia Animal do municipio de Caruaru, Estado de Pernambuco, Brasil. Fragmentos de vulva, vagina, cervix, corpo do utero, corno do utero e ovarios foram avaliados por descricao histopatologica, imuno-histoquimica (IHQ) e deteccao de DNA de formas amastigotas por qPCR. A relacao entre IHQ e infiltrado inflamatorio cronico relacionado com L. infantum foi observada apenas na vulva e vagina, enquanto as mesmas reacoes sem imunomarcacao foram observadas em todos os orgaos, exceto nos ovarios. DNA de L. infantum foi detectado em todos os orgaos do sistema genital, porem, sem diferenca de carga parasitaria entre eles. Nao houve correlacao entre a carga parasitaria e
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Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated. Key words: canine visceral leishmaniasis, genital pathology, immunohistochemistry, qPCR. Considerando a falta de estudos sobre lesoes nos orgaos genitais de cadelas naturalmente infectadas por Leishmania infantum, o objetivo do presente estudo foi investigar a correlacao entre reacao inflamatoria crocanica, imunomarcacao e carga parasitaria, no sistema genital. Dez animais foram fornecidos pelo Departamento de Controle de Vetores e Vigilancia Animal do municipio de Caruaru, Estado de Pernambuco, Brasil. Fragmentos de vulva, vagina, cervix, corpo do utero, corno do utero e ovarios foram avaliados por descricao histopatologica, imuno-histoquimica (IHQ) e deteccao de DNA de formas amastigotas por qPCR. A relacao entre IHQ e infiltrado inflamatorio cronico relacionado com L. infantum foi observada apenas na vulva e vagina, enquanto as mesmas reacoes sem imunomarcacao foram observadas em todos os orgaos, exceto nos ovarios. DNA de L. infantum foi detectado em todos os orgaos do sistema genital, porem, sem diferenca de carga parasitaria entre eles. Nao houve correlacao entre a carga parasitaria e lesoes inflamatorias nos orgaos avaliados, com excecao do corpo do utero, em que foi encontrada uma correlacao inversa. Em conclusao, a vulva e a vagina foram os principais locais de lesoes e imunomarcacao para formas amastigotas L. infantum no sistema genital de cadelas. A carga parasitaria nao influenciou a intensidade das lesoes nos orgas avaliados. Palavras-chave: leishmaniose visceral canina, patologia genital, imunohistoquimica, qPCR.</description><identifier>ISSN: 0103-8478</identifier><identifier>DOI: 10.1590/0103-8478cr20160137</identifier><language>spa</language><publisher>Universidade Federal de Santa Maria</publisher><ispartof>Ciência rural, 2016-11, p.2029</ispartof><rights>COPYRIGHT 2016 Universidade Federal de Santa Maria</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Gomes de Oliveira, Vinicius Vasconcelos</creatorcontrib><creatorcontrib>Barros de Macedo, Simone Regina</creatorcontrib><creatorcontrib>de Araujo, Flabio Ribeiro</creatorcontrib><creatorcontrib>do Nascimento Ramos, Carlos Alberto</creatorcontrib><creatorcontrib>Nascimento Ramos, Rafael Antonio</creatorcontrib><creatorcontrib>Lyra Maia, Frederico Celso</creatorcontrib><creatorcontrib>Alves, Leucio Camara</creatorcontrib><creatorcontrib>da Silva, Jr., Valdemiro Amaro</creatorcontrib><title>Correlation between chronic inflamation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum/Correlacao entre inflamacao cronica, imunomarcacao e carga parasitaria no sistema genital de cadelas naturalmente infectadas com Leishmania infantum</title><title>Ciência rural</title><description>The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum. Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated. Key words: canine visceral leishmaniasis, genital pathology, immunohistochemistry, qPCR. Considerando a falta de estudos sobre lesoes nos orgaos genitais de cadelas naturalmente infectadas por Leishmania infantum, o objetivo do presente estudo foi investigar a correlacao entre reacao inflamatoria crocanica, imunomarcacao e carga parasitaria, no sistema genital. Dez animais foram fornecidos pelo Departamento de Controle de Vetores e Vigilancia Animal do municipio de Caruaru, Estado de Pernambuco, Brasil. Fragmentos de vulva, vagina, cervix, corpo do utero, corno do utero e ovarios foram avaliados por descricao histopatologica, imuno-histoquimica (IHQ) e deteccao de DNA de formas amastigotas por qPCR. A relacao entre IHQ e infiltrado inflamatorio cronico relacionado com L. infantum foi observada apenas na vulva e vagina, enquanto as mesmas reacoes sem imunomarcacao foram observadas em todos os orgaos, exceto nos ovarios. DNA de L. infantum foi detectado em todos os orgaos do sistema genital, porem, sem diferenca de carga parasitaria entre eles. Nao houve correlacao entre a carga parasitaria e lesoes inflamatorias nos orgaos avaliados, com excecao do corpo do utero, em que foi encontrada uma correlacao inversa. Em conclusao, a vulva e a vagina foram os principais locais de lesoes e imunomarcacao para formas amastigotas L. infantum no sistema genital de cadelas. A carga parasitaria nao influenciou a intensidade das lesoes nos orgas avaliados. 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Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions without immunostaining were observed in all organs, except the ovaries. L. Infantum DNA was detected in all organs of genital system, with no difference in parasite load observed among the different organs. No correlation was reported between parasite load and inflammatory lesions in the organs evaluated, except for the uterine body, in which an inverse correlation was detected. In conclusion, the vulva and vagina were the major sites of lesions and immunostaining for L. Infantum amastigotes in the genital system of female dogs. Moreover, parasite load exerted no influence on the intensity of the lesions in the organs evaluated. Key words: canine visceral leishmaniasis, genital pathology, immunohistochemistry, qPCR. Considerando a falta de estudos sobre lesoes nos orgaos genitais de cadelas naturalmente infectadas por Leishmania infantum, o objetivo do presente estudo foi investigar a correlacao entre reacao inflamatoria crocanica, imunomarcacao e carga parasitaria, no sistema genital. Dez animais foram fornecidos pelo Departamento de Controle de Vetores e Vigilancia Animal do municipio de Caruaru, Estado de Pernambuco, Brasil. Fragmentos de vulva, vagina, cervix, corpo do utero, corno do utero e ovarios foram avaliados por descricao histopatologica, imuno-histoquimica (IHQ) e deteccao de DNA de formas amastigotas por qPCR. A relacao entre IHQ e infiltrado inflamatorio cronico relacionado com L. infantum foi observada apenas na vulva e vagina, enquanto as mesmas reacoes sem imunomarcacao foram observadas em todos os orgaos, exceto nos ovarios. DNA de L. infantum foi detectado em todos os orgaos do sistema genital, porem, sem diferenca de carga parasitaria entre eles. Nao houve correlacao entre a carga parasitaria e lesoes inflamatorias nos orgaos avaliados, com excecao do corpo do utero, em que foi encontrada uma correlacao inversa. Em conclusao, a vulva e a vagina foram os principais locais de lesoes e imunomarcacao para formas amastigotas L. infantum no sistema genital de cadelas. A carga parasitaria nao influenciou a intensidade das lesoes nos orgas avaliados. Palavras-chave: leishmaniose visceral canina, patologia genital, imunohistoquimica, qPCR.</abstract><pub>Universidade Federal de Santa Maria</pub><doi>10.1590/0103-8478cr20160137</doi></addata></record>
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title Correlation between chronic inflamation, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum/Correlacao entre inflamacao cronica, imunomarcacao e carga parasitaria no sistema genital de cadelas naturalmente infectadas com Leishmania infantum
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