Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer
The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisens...
Gespeichert in:
| Veröffentlicht in: | Cancer gene therapy 2016-09, Vol.23 (9), p.315 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 9 |
| container_start_page | 315 |
| container_title | Cancer gene therapy |
| container_volume | 23 |
| creator | Zaimy, M A Jebali, A Bazrafshan, B Mehrtashfar, S Shabani, S Tavakoli, A Hekmatimoghaddam, S H Sarli, A Azizi, H Izadi, P Kazemi, B Shojaei, A Abdalaian, A Tavakkoly-Bazzaz, J |
| description | The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software. Thereafter, naked gold nanoparticles (NGNs) were synthesized by the reaction of 1000 mm HAuCl[sub.4] with 10 [mu]g ml[sup.-1] ascorbic acid. Next, five AOs and one anti-CD33(+)/CD34(+) aptamer were attached to NGNs through serial reactions. Later, 5 ml of heparinized blood samples from five AML-M2 patients were prepared, cancerous cells were isolated and then incubated with three concentrations (75, 150 and 300 [mu]g ml[sup.-1]) each of FGNs, NGNs, gold nanoparticles functionalized with scrambled oligonucleotides (GNFSONs) and doxorubicin. Finally, cell death percentage and gene expressions were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real-time PCR, respectively. This study showed that FGNs and doxorubicin led to more cell death compared with NGNs and GNFSONs (P [less than] 0.05). Interestingly, all concentrations of FGNs led to a decrease in gene expression. As an important finding, although all concentrations of doxorubicin could also inhibit the expression of genes, FGNs had more effect (P [less than] 0.05). Moreover, both NGNs and GNFSONs could silence all genes only at a concentration of 300 [mu]g ml[sup.-1]. For BCL2 and XIAP, a dose-dependent pattern was observed, but there was no similar pattern for others. Cancer Gene Therapy (2016) 23, 315-320; doi: 10.1038/cgt.2016.33; published online 12 August 2016 |
| doi_str_mv | 10.1038/cgt.2016.33 |
| format | Article |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracmisc_A464112939</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A464112939</galeid><sourcerecordid>A464112939</sourcerecordid><originalsourceid>FETCH-LOGICAL-g679-accaae09418d0f4b40243657f1a18745c9a52c859aecab6012d80cead5c95dac3</originalsourceid><addsrcrecordid>eNptkMtOxDAMRbsAieeKH7DEukPS9JUlGp4SiM3skZu4xdBJRk0KDN_ERxIECxbIC0v2uUeWs-xEioUUqj0zQ1wUQtYLpXayfaELnUst1F52EMKzELJsGrWffS49uyfuOLJ34HvwrzTR-2aiEMjCQI4CsAM0cyRYb2n0bGGk-YXWjBDmLm43BPcFdFsY_GjBofMbnCKbMUX72ZlvNY78kXxvHJ-g51cCdJEDuUDgRx68mxPuI9uUQWfBux8kX14oBbiJuKbpKNvtcQx0_NsPs9XV5Wp5k989XN8uz-_yoW50jsYgktClbK3oy64URanqquklyrYpK6OxKkxbaSSDXS1kYVthCG3aVBaNOsxOf7QDjvTIrvdxQrPmYB7Py7qUstBKJ2rxD5XKps-YdH_Paf4n8AU654F0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer</title><source>Alma/SFX Local Collection</source><creator>Zaimy, M A ; Jebali, A ; Bazrafshan, B ; Mehrtashfar, S ; Shabani, S ; Tavakoli, A ; Hekmatimoghaddam, S H ; Sarli, A ; Azizi, H ; Izadi, P ; Kazemi, B ; Shojaei, A ; Abdalaian, A ; Tavakkoly-Bazzaz, J</creator><creatorcontrib>Zaimy, M A ; Jebali, A ; Bazrafshan, B ; Mehrtashfar, S ; Shabani, S ; Tavakoli, A ; Hekmatimoghaddam, S H ; Sarli, A ; Azizi, H ; Izadi, P ; Kazemi, B ; Shojaei, A ; Abdalaian, A ; Tavakkoly-Bazzaz, J</creatorcontrib><description>The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software. Thereafter, naked gold nanoparticles (NGNs) were synthesized by the reaction of 1000 mm HAuCl[sub.4] with 10 [mu]g ml[sup.-1] ascorbic acid. Next, five AOs and one anti-CD33(+)/CD34(+) aptamer were attached to NGNs through serial reactions. Later, 5 ml of heparinized blood samples from five AML-M2 patients were prepared, cancerous cells were isolated and then incubated with three concentrations (75, 150 and 300 [mu]g ml[sup.-1]) each of FGNs, NGNs, gold nanoparticles functionalized with scrambled oligonucleotides (GNFSONs) and doxorubicin. Finally, cell death percentage and gene expressions were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real-time PCR, respectively. This study showed that FGNs and doxorubicin led to more cell death compared with NGNs and GNFSONs (P [less than] 0.05). Interestingly, all concentrations of FGNs led to a decrease in gene expression. As an important finding, although all concentrations of doxorubicin could also inhibit the expression of genes, FGNs had more effect (P [less than] 0.05). Moreover, both NGNs and GNFSONs could silence all genes only at a concentration of 300 [mu]g ml[sup.-1]. For BCL2 and XIAP, a dose-dependent pattern was observed, but there was no similar pattern for others. Cancer Gene Therapy (2016) 23, 315-320; doi: 10.1038/cgt.2016.33; published online 12 August 2016</description><identifier>ISSN: 0929-1903</identifier><identifier>DOI: 10.1038/cgt.2016.33</identifier><language>eng</language><publisher>Nature Publishing Group</publisher><subject>Acute myelocytic leukemia ; Antisense drugs ; Care and treatment ; Dosage and administration ; Gene expression ; Genetic aspects ; Nanoparticles</subject><ispartof>Cancer gene therapy, 2016-09, Vol.23 (9), p.315</ispartof><rights>COPYRIGHT 2016 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids></links><search><creatorcontrib>Zaimy, M A</creatorcontrib><creatorcontrib>Jebali, A</creatorcontrib><creatorcontrib>Bazrafshan, B</creatorcontrib><creatorcontrib>Mehrtashfar, S</creatorcontrib><creatorcontrib>Shabani, S</creatorcontrib><creatorcontrib>Tavakoli, A</creatorcontrib><creatorcontrib>Hekmatimoghaddam, S H</creatorcontrib><creatorcontrib>Sarli, A</creatorcontrib><creatorcontrib>Azizi, H</creatorcontrib><creatorcontrib>Izadi, P</creatorcontrib><creatorcontrib>Kazemi, B</creatorcontrib><creatorcontrib>Shojaei, A</creatorcontrib><creatorcontrib>Abdalaian, A</creatorcontrib><creatorcontrib>Tavakkoly-Bazzaz, J</creatorcontrib><title>Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer</title><title>Cancer gene therapy</title><description>The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software. Thereafter, naked gold nanoparticles (NGNs) were synthesized by the reaction of 1000 mm HAuCl[sub.4] with 10 [mu]g ml[sup.-1] ascorbic acid. Next, five AOs and one anti-CD33(+)/CD34(+) aptamer were attached to NGNs through serial reactions. Later, 5 ml of heparinized blood samples from five AML-M2 patients were prepared, cancerous cells were isolated and then incubated with three concentrations (75, 150 and 300 [mu]g ml[sup.-1]) each of FGNs, NGNs, gold nanoparticles functionalized with scrambled oligonucleotides (GNFSONs) and doxorubicin. Finally, cell death percentage and gene expressions were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real-time PCR, respectively. This study showed that FGNs and doxorubicin led to more cell death compared with NGNs and GNFSONs (P [less than] 0.05). Interestingly, all concentrations of FGNs led to a decrease in gene expression. As an important finding, although all concentrations of doxorubicin could also inhibit the expression of genes, FGNs had more effect (P [less than] 0.05). Moreover, both NGNs and GNFSONs could silence all genes only at a concentration of 300 [mu]g ml[sup.-1]. For BCL2 and XIAP, a dose-dependent pattern was observed, but there was no similar pattern for others. Cancer Gene Therapy (2016) 23, 315-320; doi: 10.1038/cgt.2016.33; published online 12 August 2016</description><subject>Acute myelocytic leukemia</subject><subject>Antisense drugs</subject><subject>Care and treatment</subject><subject>Dosage and administration</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Nanoparticles</subject><issn>0929-1903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNptkMtOxDAMRbsAieeKH7DEukPS9JUlGp4SiM3skZu4xdBJRk0KDN_ERxIECxbIC0v2uUeWs-xEioUUqj0zQ1wUQtYLpXayfaELnUst1F52EMKzELJsGrWffS49uyfuOLJ34HvwrzTR-2aiEMjCQI4CsAM0cyRYb2n0bGGk-YXWjBDmLm43BPcFdFsY_GjBofMbnCKbMUX72ZlvNY78kXxvHJ-g51cCdJEDuUDgRx68mxPuI9uUQWfBux8kX14oBbiJuKbpKNvtcQx0_NsPs9XV5Wp5k989XN8uz-_yoW50jsYgktClbK3oy64URanqquklyrYpK6OxKkxbaSSDXS1kYVthCG3aVBaNOsxOf7QDjvTIrvdxQrPmYB7Py7qUstBKJ2rxD5XKps-YdH_Paf4n8AU654F0</recordid><startdate>20160901</startdate><enddate>20160901</enddate><creator>Zaimy, M A</creator><creator>Jebali, A</creator><creator>Bazrafshan, B</creator><creator>Mehrtashfar, S</creator><creator>Shabani, S</creator><creator>Tavakoli, A</creator><creator>Hekmatimoghaddam, S H</creator><creator>Sarli, A</creator><creator>Azizi, H</creator><creator>Izadi, P</creator><creator>Kazemi, B</creator><creator>Shojaei, A</creator><creator>Abdalaian, A</creator><creator>Tavakkoly-Bazzaz, J</creator><general>Nature Publishing Group</general><scope/></search><sort><creationdate>20160901</creationdate><title>Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer</title><author>Zaimy, M A ; Jebali, A ; Bazrafshan, B ; Mehrtashfar, S ; Shabani, S ; Tavakoli, A ; Hekmatimoghaddam, S H ; Sarli, A ; Azizi, H ; Izadi, P ; Kazemi, B ; Shojaei, A ; Abdalaian, A ; Tavakkoly-Bazzaz, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g679-accaae09418d0f4b40243657f1a18745c9a52c859aecab6012d80cead5c95dac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Acute myelocytic leukemia</topic><topic>Antisense drugs</topic><topic>Care and treatment</topic><topic>Dosage and administration</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Nanoparticles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zaimy, M A</creatorcontrib><creatorcontrib>Jebali, A</creatorcontrib><creatorcontrib>Bazrafshan, B</creatorcontrib><creatorcontrib>Mehrtashfar, S</creatorcontrib><creatorcontrib>Shabani, S</creatorcontrib><creatorcontrib>Tavakoli, A</creatorcontrib><creatorcontrib>Hekmatimoghaddam, S H</creatorcontrib><creatorcontrib>Sarli, A</creatorcontrib><creatorcontrib>Azizi, H</creatorcontrib><creatorcontrib>Izadi, P</creatorcontrib><creatorcontrib>Kazemi, B</creatorcontrib><creatorcontrib>Shojaei, A</creatorcontrib><creatorcontrib>Abdalaian, A</creatorcontrib><creatorcontrib>Tavakkoly-Bazzaz, J</creatorcontrib><jtitle>Cancer gene therapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zaimy, M A</au><au>Jebali, A</au><au>Bazrafshan, B</au><au>Mehrtashfar, S</au><au>Shabani, S</au><au>Tavakoli, A</au><au>Hekmatimoghaddam, S H</au><au>Sarli, A</au><au>Azizi, H</au><au>Izadi, P</au><au>Kazemi, B</au><au>Shojaei, A</au><au>Abdalaian, A</au><au>Tavakkoly-Bazzaz, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer</atitle><jtitle>Cancer gene therapy</jtitle><date>2016-09-01</date><risdate>2016</risdate><volume>23</volume><issue>9</issue><spage>315</spage><pages>315-</pages><issn>0929-1903</issn><abstract>The aim of this study was to evaluate an engineered nanostructure to silence five important oncogenes, including BAG1, MDM2, Bcl-2, BIRC5 (survivin) and XIAP, in acute myeloid leukemia subtype 2 (AML-M2). The smart nanostructures were functionalized gold nanoparticles (FGNs) containing five antisense oligonucleotides (AOs) and one anti-CD33(+)/CD34(+) aptamer. First, the best AO for each gene was selected with the OligoWalk online software, and then different arrangements of AOs were evaluated with the RNAstructure software. Thereafter, naked gold nanoparticles (NGNs) were synthesized by the reaction of 1000 mm HAuCl[sub.4] with 10 [mu]g ml[sup.-1] ascorbic acid. Next, five AOs and one anti-CD33(+)/CD34(+) aptamer were attached to NGNs through serial reactions. Later, 5 ml of heparinized blood samples from five AML-M2 patients were prepared, cancerous cells were isolated and then incubated with three concentrations (75, 150 and 300 [mu]g ml[sup.-1]) each of FGNs, NGNs, gold nanoparticles functionalized with scrambled oligonucleotides (GNFSONs) and doxorubicin. Finally, cell death percentage and gene expressions were measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and real-time PCR, respectively. This study showed that FGNs and doxorubicin led to more cell death compared with NGNs and GNFSONs (P [less than] 0.05). Interestingly, all concentrations of FGNs led to a decrease in gene expression. As an important finding, although all concentrations of doxorubicin could also inhibit the expression of genes, FGNs had more effect (P [less than] 0.05). Moreover, both NGNs and GNFSONs could silence all genes only at a concentration of 300 [mu]g ml[sup.-1]. For BCL2 and XIAP, a dose-dependent pattern was observed, but there was no similar pattern for others. Cancer Gene Therapy (2016) 23, 315-320; doi: 10.1038/cgt.2016.33; published online 12 August 2016</abstract><pub>Nature Publishing Group</pub><doi>10.1038/cgt.2016.33</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0929-1903 |
| ispartof | Cancer gene therapy, 2016-09, Vol.23 (9), p.315 |
| issn | 0929-1903 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A464112939 |
| source | Alma/SFX Local Collection |
| subjects | Acute myelocytic leukemia Antisense drugs Care and treatment Dosage and administration Gene expression Genetic aspects Nanoparticles |
| title | Coinhibition of overexpressed genes in acute myeloid leukemia subtype M2 by gold nanoparticles functionalized with five antisense oligonucleotides and one anti-CD33 aptamer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-12T05%3A13%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Coinhibition%20of%20overexpressed%20genes%20in%20acute%20myeloid%20leukemia%20subtype%20M2%20by%20gold%20nanoparticles%20functionalized%20with%20five%20antisense%20oligonucleotides%20and%20one%20anti-CD33%20aptamer&rft.jtitle=Cancer%20gene%20therapy&rft.au=Zaimy,%20M%20A&rft.date=2016-09-01&rft.volume=23&rft.issue=9&rft.spage=315&rft.pages=315-&rft.issn=0929-1903&rft_id=info:doi/10.1038/cgt.2016.33&rft_dat=%3Cgale%3EA464112939%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A464112939&rfr_iscdi=true |