Quantitative PCR Assays for Detecting Loach Minnow in the Southwestern United States

Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distribution...

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Veröffentlicht in:	PloS one 2016-09, Vol.11 (9)
Hauptverfasser:	Dysthe, Joseph C, Carim, Kellie J, Paroz, Yvette M, McKelvey, Kevin S, Young, Michael K, Schwartz, Michael K
Format:	Artikel
Sprache:	eng
Schlagworte:	Endangered species Genetic aspects Identification and classification Minnows Polymerase chain reaction Protection and preservation
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creator	Dysthe, Joseph C Carim, Kellie J Paroz, Yvette M McKelvey, Kevin S Young, Michael K Schwartz, Michael K
description	Loach minnow (Rhinichthys cobitis) and spikedace (Meda fulgida) are legally protected with the status of Endangered under the U.S. Endangered Species Act and are endemic to the Gila River basin of Arizona and New Mexico. Efficient and sensitive methods for monitoring these species' distributions are critical for prioritizing conservation efforts. We developed quantitative PCR assays for detecting loach minnow and spikedace DNA in environmental samples. Each assay reliably detected low concentrations of target DNA without detection of non-target species, including other cyprinid fishes with which they co-occur.
doi_str_mv	10.1371/journal.pone.0162200
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subjects	Endangered species Genetic aspects Identification and classification Minnows Polymerase chain reaction Protection and preservation
title	Quantitative PCR Assays for Detecting Loach Minnow in the Southwestern United States
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