Conventional and Regulatory CD4.sup.+ T Cells That Share Identical TCRs Are Derived from Common Clones

Results from studies comparing the diversity and specificity of the TCR repertoires expressed by conventional (Tconv) and regulatory (Treg) CD4.sup.+ T cell have varied depending on the experimental system employed. We developed a new model in which T cells express a single fixed TCR[alpha] chain, randomly rearranged endogenous TCR[beta] chains, and a Foxp3-GFP reporter. We purified CD4.sup.+ Foxp3.sup.- and CD4.sup.+ Foxp3.sup.+ cells, then performed biased controlled multiplex PCR and high throughput sequencing of endogenous TCR[beta] chains. We identified >7,000 different TCR[beta] sequences in the periphery of 5 individual mice. On average, ~12% of TCR sequences were expressed by both conventional and regulatory populations within individual mice. The CD4.sup.+ T cells that expressed shared TCR sequences were present at higher frequencies compared to T cells expressing non-shared TCRs. Furthermore, nearly all (>90%) of the TCR sequences that were shared within mice were identical at the DNA sequence level, indicating that conventional and regulatory T cells that express shared TCRs are derived from common clones. Analysis of TCR repertoire overlap in the thymus reveals that a large proportion of Tconv and Treg sharing observed in the periphery is due to clonal expansion in the thymus. Together these data show that there are a limited number of TCR sequences shared between Tconv and Tregs. Also, Tconv and Tregs sharing identical TCRs are found at relatively high frequencies and are derived from common progenitors, of which a large portion are generated in the thymus.