HPLC of anthocyans with an amperometric detector: Evaluation of the antioxidant activity

Anthocyan complexes of a series of grape varieties with the composition traditional for Vitis vinifera, containing 3-glucosides of five anthocyanidins, delphinidin (Dp3G), cyanidin (Cy3G), petunidin (Pt3G), peonidin (Pn3G), and malvidin (Mv3G) as the major components have been studied by reversed-phase HPLC with diode-array, mass spectrometric, and amperometric detectors. A procedure has been developed for the determination of the antioxidant activity of individual anthocyans using an amperometric method; the peculiarities of using a Tsvet Yauza-01-AA system have been evaluated. It has been shown that the sensitivity of amperometric detection depends not only on the structure of anthocyans, but also on the anode voltage. A scheme of the calculation of the antioxidant activity of anthocyan complexes by the results of amperometric detection of a mixture of individual anthocyans has been proposed.