Crocin, a dietary additive protects platelets from oxidative stress-induced apoptosis and inhibits platelet aggregation
Platelets are the key players in the development of cardiovascular diseases as the microparticles generated by apoptotic platelets and platelet aggregation contribute actively towards the disease propagation. Thus, the aim of this study was to demonstrate the effect of a phytochemical which can prev...
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| Veröffentlicht in: | Molecular and cellular biochemistry 2013-01, Vol.373 (1-2), p.73-83 |
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| creator | Thushara, R. M. Hemshekhar, M. Santhosh, M. Sebastin Jnaneshwari, S. Nayaka, S. C. Naveen, S. Kemparaju, K. Girish, K. S. |
| description | Platelets are the key players in the development of cardiovascular diseases as the microparticles generated by apoptotic platelets and platelet aggregation contribute actively towards the disease propagation. Thus, the aim of this study was to demonstrate the effect of a phytochemical which can prevent these two processes and thereby project it as a cardio-protective compound. Crocin, a natural carotenoid exhibits a wide spectrum of therapeutic potentials through its antioxidant property. The study demonstrated its effects on cytoplasmic apoptotic events of mitochondrial pathway in platelets. Collagen/calcium ionophore-A23187 stimulated platelets were treated with crocin and endogenous generation of reactive oxygen species (ROS) and hydrogen peroxide (H
2
O
2
) were measured. H
2
O
2
-induced changes in crocin-pretreated platelets such as intracellular calcium, mitochondrial membrane potential (ΔΨ
m
), caspase activity, phosphatidylserine exposure and cytochrome c translocation were determined. Crocin dose-dependently ameliorated collagen- and A23187-induced endogenous generation of ROS and H
2
O
2
. It also abolished the H
2
O
2
-induced events of intrinsic pathway of apoptosis. Further, it hindered collagen-induced platelet aggregation and adhesion. The current piece of work clearly suggests its anti-apoptotic effect as well as inhibitory effects on platelet aggregation. Thus, crocin can be deemed as a prospective candidate in the treatment regime of platelet-associated diseases.
Graphical Abstract |
| doi_str_mv | 10.1007/s11010-012-1476-7 |
| format | Article |
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2
O
2
) were measured. H
2
O
2
-induced changes in crocin-pretreated platelets such as intracellular calcium, mitochondrial membrane potential (ΔΨ
m
), caspase activity, phosphatidylserine exposure and cytochrome c translocation were determined. Crocin dose-dependently ameliorated collagen- and A23187-induced endogenous generation of ROS and H
2
O
2
. It also abolished the H
2
O
2
-induced events of intrinsic pathway of apoptosis. Further, it hindered collagen-induced platelet aggregation and adhesion. The current piece of work clearly suggests its anti-apoptotic effect as well as inhibitory effects on platelet aggregation. Thus, crocin can be deemed as a prospective candidate in the treatment regime of platelet-associated diseases.
Graphical Abstract</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/s11010-012-1476-7</identifier><identifier>PMID: 23065381</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Antioxidants ; Antioxidants - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Biochemistry ; Biomedical and Life Sciences ; Blood Platelets - drug effects ; Blood Platelets - metabolism ; Blood Platelets - physiology ; Calcium Signaling ; Cardiology ; Cardiovascular diseases ; Carotenoids - pharmacology ; Caspase 3 - metabolism ; Caspase 9 - metabolism ; Cell Adhesion - drug effects ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Collagen ; Cytochrome c ; Cytochromes c - metabolism ; Enzyme Activation - drug effects ; Food Additives - pharmacology ; Health aspects ; Humans ; Hydrogen Peroxide - metabolism ; Life Sciences ; Medical Biochemistry ; Membrane Potential, Mitochondrial - drug effects ; Oncology ; Oxidative stress ; Oxidative Stress - drug effects ; Phosphatidylserines - metabolism ; Platelet Aggregation - drug effects ; Platelet Aggregation Inhibitors - pharmacology</subject><ispartof>Molecular and cellular biochemistry, 2013-01, Vol.373 (1-2), p.73-83</ispartof><rights>Springer Science+Business Media New York 2012</rights><rights>COPYRIGHT 2013 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-fc42d63f3697e898f48cd7c7c82d008717c1995a4076ab76e2e876016120a7003</citedby><cites>FETCH-LOGICAL-c477t-fc42d63f3697e898f48cd7c7c82d008717c1995a4076ab76e2e876016120a7003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11010-012-1476-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11010-012-1476-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23065381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thushara, R. M.</creatorcontrib><creatorcontrib>Hemshekhar, M.</creatorcontrib><creatorcontrib>Santhosh, M. Sebastin</creatorcontrib><creatorcontrib>Jnaneshwari, S.</creatorcontrib><creatorcontrib>Nayaka, S. C.</creatorcontrib><creatorcontrib>Naveen, S.</creatorcontrib><creatorcontrib>Kemparaju, K.</creatorcontrib><creatorcontrib>Girish, K. S.</creatorcontrib><title>Crocin, a dietary additive protects platelets from oxidative stress-induced apoptosis and inhibits platelet aggregation</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><addtitle>Mol Cell Biochem</addtitle><description>Platelets are the key players in the development of cardiovascular diseases as the microparticles generated by apoptotic platelets and platelet aggregation contribute actively towards the disease propagation. Thus, the aim of this study was to demonstrate the effect of a phytochemical which can prevent these two processes and thereby project it as a cardio-protective compound. Crocin, a natural carotenoid exhibits a wide spectrum of therapeutic potentials through its antioxidant property. The study demonstrated its effects on cytoplasmic apoptotic events of mitochondrial pathway in platelets. Collagen/calcium ionophore-A23187 stimulated platelets were treated with crocin and endogenous generation of reactive oxygen species (ROS) and hydrogen peroxide (H
2
O
2
) were measured. H
2
O
2
-induced changes in crocin-pretreated platelets such as intracellular calcium, mitochondrial membrane potential (ΔΨ
m
), caspase activity, phosphatidylserine exposure and cytochrome c translocation were determined. Crocin dose-dependently ameliorated collagen- and A23187-induced endogenous generation of ROS and H
2
O
2
. It also abolished the H
2
O
2
-induced events of intrinsic pathway of apoptosis. Further, it hindered collagen-induced platelet aggregation and adhesion. The current piece of work clearly suggests its anti-apoptotic effect as well as inhibitory effects on platelet aggregation. Thus, crocin can be deemed as a prospective candidate in the treatment regime of platelet-associated diseases.
Graphical Abstract</description><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - metabolism</subject><subject>Blood Platelets - physiology</subject><subject>Calcium Signaling</subject><subject>Cardiology</subject><subject>Cardiovascular diseases</subject><subject>Carotenoids - pharmacology</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 9 - metabolism</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Collagen</subject><subject>Cytochrome c</subject><subject>Cytochromes c - metabolism</subject><subject>Enzyme Activation - drug effects</subject><subject>Food Additives - pharmacology</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Life Sciences</subject><subject>Medical Biochemistry</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Oncology</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Phosphatidylserines - metabolism</subject><subject>Platelet Aggregation - drug effects</subject><subject>Platelet Aggregation Inhibitors - pharmacology</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2LFDEQhoO4uLMfP8CLBLya3apOT6r7uAx-LCx40XPIJOk2y3SnSTJ-_HsztoqCSA4Vqp6nDvUy9hzhBgHoNiMCggBsBLakBD1hG9ySFG2P_VO2AQkgOiQ6Zxc5P0KFAfEZO28kqK3scMO-7FK0YX7FDXfBF5O-ceNcKOGz50uKxduS-XIwxR98_Q0pTjx-Dc78IHJJPmcRZne03nGzxKXEHDI3s-Nh_hT24Q-dm3FMfqxqnK_Y2WAO2V__rJfs45vXH3bvxMP7t_e7uwdhW6IiBts2TslBqp5813dD21lHlmzXOICOkCz2_da0QMrsSfnGd6QAFTZgCEBespfr3tEcvA7zEEsydgrZ6jsptz1J1VClbv5B1ef8FGyc_RBq_y8BV8GmmHPyg15SmOr1NII-ZaPXbHTNRp-y0Sfnxeosx_3k3W_jVxgVaFYg19E8-qQf4zHN9Tz_2fodrqCZ3Q</recordid><startdate>20130101</startdate><enddate>20130101</enddate><creator>Thushara, R. M.</creator><creator>Hemshekhar, M.</creator><creator>Santhosh, M. Sebastin</creator><creator>Jnaneshwari, S.</creator><creator>Nayaka, S. C.</creator><creator>Naveen, S.</creator><creator>Kemparaju, K.</creator><creator>Girish, K. S.</creator><general>Springer US</general><general>Springer</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20130101</creationdate><title>Crocin, a dietary additive protects platelets from oxidative stress-induced apoptosis and inhibits platelet aggregation</title><author>Thushara, R. M. ; Hemshekhar, M. ; Santhosh, M. Sebastin ; Jnaneshwari, S. ; Nayaka, S. C. ; Naveen, S. ; Kemparaju, K. ; Girish, K. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-fc42d63f3697e898f48cd7c7c82d008717c1995a4076ab76e2e876016120a7003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - metabolism</topic><topic>Blood Platelets - physiology</topic><topic>Calcium Signaling</topic><topic>Cardiology</topic><topic>Cardiovascular diseases</topic><topic>Carotenoids - pharmacology</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 9 - metabolism</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Collagen</topic><topic>Cytochrome c</topic><topic>Cytochromes c - metabolism</topic><topic>Enzyme Activation - drug effects</topic><topic>Food Additives - pharmacology</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Life Sciences</topic><topic>Medical Biochemistry</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Oncology</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Phosphatidylserines - metabolism</topic><topic>Platelet Aggregation - drug effects</topic><topic>Platelet Aggregation Inhibitors - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thushara, R. M.</creatorcontrib><creatorcontrib>Hemshekhar, M.</creatorcontrib><creatorcontrib>Santhosh, M. Sebastin</creatorcontrib><creatorcontrib>Jnaneshwari, S.</creatorcontrib><creatorcontrib>Nayaka, S. C.</creatorcontrib><creatorcontrib>Naveen, S.</creatorcontrib><creatorcontrib>Kemparaju, K.</creatorcontrib><creatorcontrib>Girish, K. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thushara, R. M.</au><au>Hemshekhar, M.</au><au>Santhosh, M. Sebastin</au><au>Jnaneshwari, S.</au><au>Nayaka, S. C.</au><au>Naveen, S.</au><au>Kemparaju, K.</au><au>Girish, K. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crocin, a dietary additive protects platelets from oxidative stress-induced apoptosis and inhibits platelet aggregation</atitle><jtitle>Molecular and cellular biochemistry</jtitle><stitle>Mol Cell Biochem</stitle><addtitle>Mol Cell Biochem</addtitle><date>2013-01-01</date><risdate>2013</risdate><volume>373</volume><issue>1-2</issue><spage>73</spage><epage>83</epage><pages>73-83</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Platelets are the key players in the development of cardiovascular diseases as the microparticles generated by apoptotic platelets and platelet aggregation contribute actively towards the disease propagation. Thus, the aim of this study was to demonstrate the effect of a phytochemical which can prevent these two processes and thereby project it as a cardio-protective compound. Crocin, a natural carotenoid exhibits a wide spectrum of therapeutic potentials through its antioxidant property. The study demonstrated its effects on cytoplasmic apoptotic events of mitochondrial pathway in platelets. Collagen/calcium ionophore-A23187 stimulated platelets were treated with crocin and endogenous generation of reactive oxygen species (ROS) and hydrogen peroxide (H
2
O
2
) were measured. H
2
O
2
-induced changes in crocin-pretreated platelets such as intracellular calcium, mitochondrial membrane potential (ΔΨ
m
), caspase activity, phosphatidylserine exposure and cytochrome c translocation were determined. Crocin dose-dependently ameliorated collagen- and A23187-induced endogenous generation of ROS and H
2
O
2
. It also abolished the H
2
O
2
-induced events of intrinsic pathway of apoptosis. Further, it hindered collagen-induced platelet aggregation and adhesion. The current piece of work clearly suggests its anti-apoptotic effect as well as inhibitory effects on platelet aggregation. Thus, crocin can be deemed as a prospective candidate in the treatment regime of platelet-associated diseases.
Graphical Abstract</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>23065381</pmid><doi>10.1007/s11010-012-1476-7</doi><tpages>11</tpages></addata></record> |
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| ispartof | Molecular and cellular biochemistry, 2013-01, Vol.373 (1-2), p.73-83 |
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| subjects | Antioxidants Antioxidants - pharmacology Apoptosis Apoptosis - drug effects Biochemistry Biomedical and Life Sciences Blood Platelets - drug effects Blood Platelets - metabolism Blood Platelets - physiology Calcium Signaling Cardiology Cardiovascular diseases Carotenoids - pharmacology Caspase 3 - metabolism Caspase 9 - metabolism Cell Adhesion - drug effects Cell Membrane - drug effects Cell Membrane - metabolism Collagen Cytochrome c Cytochromes c - metabolism Enzyme Activation - drug effects Food Additives - pharmacology Health aspects Humans Hydrogen Peroxide - metabolism Life Sciences Medical Biochemistry Membrane Potential, Mitochondrial - drug effects Oncology Oxidative stress Oxidative Stress - drug effects Phosphatidylserines - metabolism Platelet Aggregation - drug effects Platelet Aggregation Inhibitors - pharmacology |
| title | Crocin, a dietary additive protects platelets from oxidative stress-induced apoptosis and inhibits platelet aggregation |
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