Alterations of cytological and karyological profile of human mesenchymal stem cells during in vitro culturing
Transplantation of human bone marrow mesenchymal stem cells is considered as a promising therapeutic approach to the therapy of many diseases. However, the problem of possible alterations of the properties of mesenchymal stem cells during their expansion in in vitro cultures before transplantation i...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2010-12, Vol.150 (1), p.125 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Grigorian, A S Kruglyakov, P V Taminkina, U A Efimova, O A Pendina, A A Voskresenskaya, A V Kuznetsova, T V Polyntsev, D G |
| description | Transplantation of human bone marrow mesenchymal stem cells is considered as a promising therapeutic approach to the therapy of many diseases. However, the problem of possible alterations of the properties of mesenchymal stem cells during their expansion in in vitro cultures before transplantation is not solved. In our study, one of two hundred examined cultures of mesenchymal stem cell cultures derived from donors without bone marrow pathologies and developed under standard culturing conditions demonstrated spontaneous disturbances in morphology, proliferation, and karyotype at early passages. The cells of this abnormal culture retained immunophenotype characteristic of normal mesenchymal stem cells, but some of them (15-25%) had numerous numerical and structural chromosome aberrations. |
| doi_str_mv | 10.1007/s10517-010-1086-x |
| format | Article |
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| subjects | Cell Culture Techniques - methods Cells, Cultured Chromosome Aberrations Chromosomes Health aspects Humans Immunophenotyping Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - metabolism Stem cells |
| title | Alterations of cytological and karyological profile of human mesenchymal stem cells during in vitro culturing |
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