Evaluation of the immunosuppressive effect of cyclophosphamide and dexamethasone in mice with visceral toxocariasis

Visceral toxocariasis is a serious public health problem with a cosmopolitan distribution. Children are susceptible due to their immature immune system and high risks of infection. Nevertheless, the few completed studies about immunosuppression have had controversial results. To evaluate the effect...

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Veröffentlicht in:Parasitology research (1987) 2012, Vol.110 (1), p.443-447
Hauptverfasser: da Costa de Avila, Luciana Farias, da Fonseca, Juliana Santos Vieira, Dutra, Gisele Ferreira, de Lima Telmo, Paula, Silva, Ana Maria Wolkmer Azambuja, Berne, Maria Elisabeth Aires, da Silva, Pedro Eduardo Almeida, Conceição, Fabricio Rochedo, Scaini, Carlos James
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container_issue 1
container_start_page 443
container_title Parasitology research (1987)
container_volume 110
creator da Costa de Avila, Luciana Farias
da Fonseca, Juliana Santos Vieira
Dutra, Gisele Ferreira
de Lima Telmo, Paula
Silva, Ana Maria Wolkmer Azambuja
Berne, Maria Elisabeth Aires
da Silva, Pedro Eduardo Almeida
Conceição, Fabricio Rochedo
Scaini, Carlos James
description Visceral toxocariasis is a serious public health problem with a cosmopolitan distribution. Children are susceptible due to their immature immune system and high risks of infection. Nevertheless, the few completed studies about immunosuppression have had controversial results. To evaluate the effect of two immunosuppressive drugs on the larval burden of Toxocara canis , four groups of ten Swiss strain mice each were inoculated on day 0 with 1,200 embryonated T. canis eggs. Fifteen days before the experimental infection, group 1 (control) was treated via intraperitoneal injection (IP) with sterile distilled water and groups 2 and 3 were treated with dexamethasone (DEX) at 1 and 5 mg/kg/day, respectively. Additionally, group 4 was treated IP with cyclophosphamide (CY) at 50 mg/kg at two times per week for 2 weeks. Sixty days following infection, the mice were euthanised to recover the larvae by means of the tissue digestion technique. The levels of antibodies detected by indirect ELISA were not associated with the larval burden. Administration of CY (50 mg/kg) and DEX (5 mg/kg) resulted in an increase of the larval burden of 162.1% and 50.8%, respectively, in relation to the control group. These two treatments, especially CY (50 mg/kg), promoted immunosuppression and the establishment of a significant larval burden, supporting its further utilisation in studies related to immunosuppression in visceral toxocariasis.
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subjects Animal Structures - parasitology
Animals
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Cyclophosphamide
Cyclophosphamide - administration & dosage
Development and progression
Dexamethasone
Dexamethasone - administration & dosage
Female
Fundamental and applied biological sciences. Psychology
General aspects
General aspects and techniques. Study of several systematic groups. Models
Health aspects
Immunology
Immunosuppressive Agents - administration & dosage
Invertebrates
Larva - immunology
Larva - pathogenicity
Medical Microbiology
Mice
Microbiology
Original Paper
Parasite Load
Physiological aspects
Roundworm infections
Toxocara canis - immunology
Toxocara canis - isolation & purification
Toxocara canis - pathogenicity
Toxocariasis - immunology
Toxocariasis - parasitology
title Evaluation of the immunosuppressive effect of cyclophosphamide and dexamethasone in mice with visceral toxocariasis
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