Alterations in the osmotic fragility of camel and donkey erythrocytes caused by temperature, pH and blood storage/Promjene u osmotskoj otpornosti eritrocita deve i magarca uzrokovane temperaturom, pH i pohranom krvi

The present study examined the changes in the fragility of camel and donkey erythrocytes caused by variations in temperature, pH and blood storage. Although the fragility did not differ between sexes in both species, camel erythrocytes were more osmotically resistant than donkey erythrocytes. At a constant pH of 7.4, the fragility decreased in the camel but increased in the donkey at lowered temperatures. However, at a constant temperature of 28°C, the fragility of camel and donkey erythrocytes increased in an acidic solution and decreased in an alkaline solution. Additionally, blood storage for 24 hours at 4°C increased the fragility of camel erythrocytes, but not donkey erythrocytes. However, the erythrocyte fragility increased in both species after 48 or 72 hours of blood storage at 4°C. In conclusion, our results suggest that variation in the temperature and pH of the erythrocyte environment and duration of blood storage may each play a significant role in the osmotic behaviour of camel and donkey erythrocytes. Key words: camels, donkeys, erythrocyte fragility, temperature, pH, blood storage Cilj istrazivanja bio je ustanoviti razliku u osmotskoj otpornosti eritrocita deve i magarca koja se javlja pod utjecajem temperature, pH i trajanja pohrane krvi. Iako nije ustanovljena razlika izmedu muskoga i zenskoga spola u istrazivanih vrsta, eritrociti deve pokazali su se osmotski otpornijima u odnosu na eritrocite magarca. Pri stalnoj pH vrijednosti od 7,4 otpornost eritrocita deve se smanjila, a eritrocita magarca povecala uz snizenu temperaturu. Pri stalnoj temperaturi od 28°C otpornost eritrocita deve i magarca povecala se u kiseloj, a smanjila u luznatoj otopini. Pohranom krvi tijekom 24 sata na 4°C povecala se krhkost eritrocita deve, ali ne i eritrocita magarca. No krhkost eritrocita obiju vrsta povecala se tijekom pohrane od 48 do 72 sata na temperaturi od 4°C. Na temelju rezultata moze se zakljuciti da razlike u temperaturi i pH medija te duljina pohrane krvi mogu imati znacajnu ulogu u osmotskoj otpornosti eritrocita deve i magarca. Kljucne rijeci: deve, magarci, otpornost eritrocita, temperatura, pH, pohrana krvi