Photoactivation of the [CreER.sup.T2] recombinase for conditional site-specific recombination with high spatiotemporal resolution

We implemented a noninvasive optical method for the fast control of Cre recombinase in single cells of a live zebrafish embryo. Optical uncaging of the caged precursor of a nonendogeneous steroid by one- or two-photon illumination was used to restore Cre activity of the [CreER.sup.T2] fusion protein...

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Veröffentlicht in:	Zebrafish 2010-06, Vol.7 (2), p.199
Hauptverfasser:	Sinha, Deepak Kumar, Neveu, Pierre, Gagey, Nathalie, Aujard, Isabelle, Le Saux, Thomas, Rampon, Christine, Gauron, Carole, Kawakami, Koichi, Leucht, Christoph, Bally-Cuif, Laure, Volovitch, Michel, Bensimon, David, Jullien, Ludovic, Vriz, Sophie
Format:	Artikel
Sprache:	eng
Schlagworte:	Gene expression Genetic aspects Physiological aspects Recombinases Zebra fish
Online-Zugang:	Volltext
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