Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey
Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs),...
Gespeichert in:
| Veröffentlicht in: | Foodborne pathogens and disease 2009-10, Vol.6 (8), p.1029-1035 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1035 |
|---|---|
| container_issue | 8 |
| container_start_page | 1029 |
| container_title | Foodborne pathogens and disease |
| container_volume | 6 |
| creator | Karahan, Murat Acik, Mehmet Nuri Cetinkaya, Burhan |
| description | Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n=92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies. |
| doi_str_mv | 10.1089/fpd.2009.0304 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_cross</sourceid><recordid>TN_cdi_gale_infotracmisc_A211555034</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A211555034</galeid><sourcerecordid>A211555034</sourcerecordid><originalsourceid>FETCH-LOGICAL-c383t-8b0873a52ca0f134263cedb702121fd9642f50b7bbc76242b475286e5d9f5deb3</originalsourceid><addsrcrecordid>eNptkUtv1DAURi0Eog9YsgVLrDNcv_JYllEpIxWBmOnacpzrqSGJR3amIlt-eR2mEkJCXtwr-3x-HULeMFgxqJsP7tCtOECzAgHyGTlnSpVFxbh6vvRCFYJJdkYuUvoBwBuuqpfkjDWl5A1T5-T3ZnzANPm9mXwYaXB0F375kd7giIm2M_0W-nnAaBLS9b3JK9_R2D9s7reTOdzPfbDB2mOi5hgxl-0UM5joJoXeTNhRF8NAP4YHPyL9YvJpk09LfHeMP3F-RV440yd8_VQvyd2n6936c3H79WazvrotrKjFVNQt1JUwilsDjgnJS2GxayvgjDPXLS9yCtqqbW1VcslbWSlel6i6xqkOW3FJ3p_23ZsetR9dyPe0g09WX3GW_02BkJla_YfKo8PB2zCi83n-n0BxCtgYUoro9CH6wcRZM9CLIp0V6UWRXhRl_u2JPxzbAbu_9JOTDLw7Ac4EbfbRJ3235cAEsAqkbEA8Aj_HliQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Karahan, Murat ; Acik, Mehmet Nuri ; Cetinkaya, Burhan</creator><creatorcontrib>Karahan, Murat ; Acik, Mehmet Nuri ; Cetinkaya, Burhan</creatorcontrib><description>Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n=92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies.</description><identifier>ISSN: 1535-3141</identifier><identifier>EISSN: 1556-7125</identifier><identifier>DOI: 10.1089/fpd.2009.0304</identifier><identifier>PMID: 19642915</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Bacterial toxins ; Bacterial Toxins - genetics ; Bacterial Toxins - metabolism ; Bacterial Typing Techniques ; beef cattle ; bovine mastitis ; Cattle ; Causes of ; Coagulase - metabolism ; Diagnosis ; Electrophoresis, Agar Gel ; enterotoxins ; Enterotoxins - genetics ; Enterotoxins - metabolism ; etiology ; Exfoliatins - genetics ; Exfoliatins - metabolism ; Female ; Food Microbiology ; food pathogens ; Foodborne Diseases - prevention & control ; genes ; Genetic aspects ; Genetic Variation ; isolation ; Mastitis ; Mastitis, Bovine - microbiology ; Physiological aspects ; Polymerase Chain Reaction ; septic shock ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; staphylococcal intoxication ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus aureus - metabolism ; Superantigens - genetics ; Superantigens - metabolism ; toxic shock syndrome toxin ; toxigenic strains ; Turkey ; virulence</subject><ispartof>Foodborne pathogens and disease, 2009-10, Vol.6 (8), p.1029-1035</ispartof><rights>COPYRIGHT 2009 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-8b0873a52ca0f134263cedb702121fd9642f50b7bbc76242b475286e5d9f5deb3</citedby><cites>FETCH-LOGICAL-c383t-8b0873a52ca0f134263cedb702121fd9642f50b7bbc76242b475286e5d9f5deb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19642915$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Karahan, Murat</creatorcontrib><creatorcontrib>Acik, Mehmet Nuri</creatorcontrib><creatorcontrib>Cetinkaya, Burhan</creatorcontrib><title>Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey</title><title>Foodborne pathogens and disease</title><addtitle>Foodborne Pathog Dis</addtitle><description>Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n=92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies.</description><subject>Animals</subject><subject>Bacterial toxins</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacterial Typing Techniques</subject><subject>beef cattle</subject><subject>bovine mastitis</subject><subject>Cattle</subject><subject>Causes of</subject><subject>Coagulase - metabolism</subject><subject>Diagnosis</subject><subject>Electrophoresis, Agar Gel</subject><subject>enterotoxins</subject><subject>Enterotoxins - genetics</subject><subject>Enterotoxins - metabolism</subject><subject>etiology</subject><subject>Exfoliatins - genetics</subject><subject>Exfoliatins - metabolism</subject><subject>Female</subject><subject>Food Microbiology</subject><subject>food pathogens</subject><subject>Foodborne Diseases - prevention & control</subject><subject>genes</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>isolation</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction</subject><subject>septic shock</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>staphylococcal intoxication</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Superantigens - genetics</subject><subject>Superantigens - metabolism</subject><subject>toxic shock syndrome toxin</subject><subject>toxigenic strains</subject><subject>Turkey</subject><subject>virulence</subject><issn>1535-3141</issn><issn>1556-7125</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtv1DAURi0Eog9YsgVLrDNcv_JYllEpIxWBmOnacpzrqSGJR3amIlt-eR2mEkJCXtwr-3x-HULeMFgxqJsP7tCtOECzAgHyGTlnSpVFxbh6vvRCFYJJdkYuUvoBwBuuqpfkjDWl5A1T5-T3ZnzANPm9mXwYaXB0F375kd7giIm2M_0W-nnAaBLS9b3JK9_R2D9s7reTOdzPfbDB2mOi5hgxl-0UM5joJoXeTNhRF8NAP4YHPyL9YvJpk09LfHeMP3F-RV440yd8_VQvyd2n6936c3H79WazvrotrKjFVNQt1JUwilsDjgnJS2GxayvgjDPXLS9yCtqqbW1VcslbWSlel6i6xqkOW3FJ3p_23ZsetR9dyPe0g09WX3GW_02BkJla_YfKo8PB2zCi83n-n0BxCtgYUoro9CH6wcRZM9CLIp0V6UWRXhRl_u2JPxzbAbu_9JOTDLw7Ac4EbfbRJ3235cAEsAqkbEA8Aj_HliQ</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Karahan, Murat</creator><creator>Acik, Mehmet Nuri</creator><creator>Cetinkaya, Burhan</creator><general>Mary Ann Liebert, Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20091001</creationdate><title>Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey</title><author>Karahan, Murat ; Acik, Mehmet Nuri ; Cetinkaya, Burhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-8b0873a52ca0f134263cedb702121fd9642f50b7bbc76242b475286e5d9f5deb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacterial toxins</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Toxins - metabolism</topic><topic>Bacterial Typing Techniques</topic><topic>beef cattle</topic><topic>bovine mastitis</topic><topic>Cattle</topic><topic>Causes of</topic><topic>Coagulase - metabolism</topic><topic>Diagnosis</topic><topic>Electrophoresis, Agar Gel</topic><topic>enterotoxins</topic><topic>Enterotoxins - genetics</topic><topic>Enterotoxins - metabolism</topic><topic>etiology</topic><topic>Exfoliatins - genetics</topic><topic>Exfoliatins - metabolism</topic><topic>Female</topic><topic>Food Microbiology</topic><topic>food pathogens</topic><topic>Foodborne Diseases - prevention & control</topic><topic>genes</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>isolation</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Physiological aspects</topic><topic>Polymerase Chain Reaction</topic><topic>septic shock</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>staphylococcal intoxication</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Superantigens - genetics</topic><topic>Superantigens - metabolism</topic><topic>toxic shock syndrome toxin</topic><topic>toxigenic strains</topic><topic>Turkey</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karahan, Murat</creatorcontrib><creatorcontrib>Acik, Mehmet Nuri</creatorcontrib><creatorcontrib>Cetinkaya, Burhan</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Foodborne pathogens and disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karahan, Murat</au><au>Acik, Mehmet Nuri</au><au>Cetinkaya, Burhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey</atitle><jtitle>Foodborne pathogens and disease</jtitle><addtitle>Foodborne Pathog Dis</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>6</volume><issue>8</issue><spage>1029</spage><epage>1035</epage><pages>1029-1035</pages><issn>1535-3141</issn><eissn>1556-7125</eissn><abstract>Staphylococcus aureus causes a number of diseases in humans and animals, and it is the most common etiological agent of contagious bovine mastitis. The agent produces several virulence factors such as coagulase (coa), clumping factor, protein A, exfoliative toxins, staphylococcal enterotoxins (SEs), and toxic shock syndrome toxin-1. The aim of the present study was to characterize coa-positive S. aureus strains (n=92) isolated from bovine subclinical mastitis in Turkey by polymerase chain reaction (PCR) amplification of exfoliative toxin (eta and etb) and toxic shock syndrome toxin-1 (tsst) genes. In addition, a multiplex PCR was employed to investigate the presence of SE genes sea, seb, sec, sed, see, seg, seh, sej, and sei. By PCR amplification, while eta and etb were not detected, only three isolates (3.3%) were positive for tsst. Twenty-seven (29.3%) isolates harbored one or more SE genes, and sei was the most common pattern by multiplex PCR. None of the isolates harbored the genes encoding sea, see, and seh. The application of this multiplex PCR assay could enable more samples to be rapidly characterized for enterotoxin production of S. aureus isolates from milk for epidemiological studies.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>19642915</pmid><doi>10.1089/fpd.2009.0304</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1535-3141 |
| ispartof | Foodborne pathogens and disease, 2009-10, Vol.6 (8), p.1029-1035 |
| issn | 1535-3141 1556-7125 |
| language | eng |
| recordid | cdi_gale_infotracmisc_A211555034 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bacterial toxins Bacterial Toxins - genetics Bacterial Toxins - metabolism Bacterial Typing Techniques beef cattle bovine mastitis Cattle Causes of Coagulase - metabolism Diagnosis Electrophoresis, Agar Gel enterotoxins Enterotoxins - genetics Enterotoxins - metabolism etiology Exfoliatins - genetics Exfoliatins - metabolism Female Food Microbiology food pathogens Foodborne Diseases - prevention & control genes Genetic aspects Genetic Variation isolation Mastitis Mastitis, Bovine - microbiology Physiological aspects Polymerase Chain Reaction septic shock Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary staphylococcal intoxication Staphylococcus aureus Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Staphylococcus aureus - metabolism Superantigens - genetics Superantigens - metabolism toxic shock syndrome toxin toxigenic strains Turkey virulence |
| title | Investigation of Toxin Genes by Polymerase Chain Reaction in Staphylococcus aureus Strains Isolated from Bovine Mastitis in Turkey |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T17%3A11%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Investigation%20of%20Toxin%20Genes%20by%20Polymerase%20Chain%20Reaction%20in%20Staphylococcus%20aureus%20Strains%20Isolated%20from%20Bovine%20Mastitis%20in%20Turkey&rft.jtitle=Foodborne%20pathogens%20and%20disease&rft.au=Karahan,%20Murat&rft.date=2009-10-01&rft.volume=6&rft.issue=8&rft.spage=1029&rft.epage=1035&rft.pages=1029-1035&rft.issn=1535-3141&rft.eissn=1556-7125&rft_id=info:doi/10.1089/fpd.2009.0304&rft_dat=%3Cgale_cross%3EA211555034%3C/gale_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/19642915&rft_galeid=A211555034&rfr_iscdi=true |