Antimutagenicity of hops ( Humulus lupulus L.): bioassay-directed fractionation and isolation of xanthohumol
Bioassay-directed fractionation with a Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5- f]quinoline (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by I...
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| Veröffentlicht in: | Phytomedicine (Stuttgart) 2008-03, Vol.15 (3), p.216-220 |
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| creator | Kac, Javor Plazar, Janja Mlinarič, Aleš Žegura, Bojana Lah, Tamara T. Filipič, Metka |
| description | Bioassay-directed fractionation with a
Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5-
f]quinoline (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by IQ. Fractionation of the diethylether extract (DE) by column chromatography, followed by semi-preparative HPLC yielded two fractions (E4b and E4d) with strong antimutagenic activity against IQ induced mutations. Separation of fraction E4b resulted in inactive fractions, while fraction E4d has been identified to be xanthohumol. In mammalian test system with human hepatoma HepG2 cells fraction E4d at 10
μg/ml completely prevented formation of IQ induced DNA damage. These results indicate that xanthohumol is a very promising potential protective agent against genotoxicity of food borne carcinogens, which warrants further investigation. |
| doi_str_mv | 10.1016/j.phymed.2007.09.008 |
| format | Article |
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Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5-
f]quinoline (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by IQ. Fractionation of the diethylether extract (DE) by column chromatography, followed by semi-preparative HPLC yielded two fractions (E4b and E4d) with strong antimutagenic activity against IQ induced mutations. Separation of fraction E4b resulted in inactive fractions, while fraction E4d has been identified to be xanthohumol. In mammalian test system with human hepatoma HepG2 cells fraction E4d at 10
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Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5-
f]quinoline (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by IQ. Fractionation of the diethylether extract (DE) by column chromatography, followed by semi-preparative HPLC yielded two fractions (E4b and E4d) with strong antimutagenic activity against IQ induced mutations. Separation of fraction E4b resulted in inactive fractions, while fraction E4d has been identified to be xanthohumol. In mammalian test system with human hepatoma HepG2 cells fraction E4d at 10
μg/ml completely prevented formation of IQ induced DNA damage. These results indicate that xanthohumol is a very promising potential protective agent against genotoxicity of food borne carcinogens, which warrants further investigation.</description><subject>4-Nitroquinoline-1-oxide - toxicity</subject><subject>Animals</subject><subject>Antimutagen</subject><subject>Antimutagenic Agents - chemistry</subject><subject>Antimutagenic Agents - isolation & purification</subject><subject>Antimutagenic Agents - pharmacology</subject><subject>Antioxidants</subject><subject>Biological assay</subject><subject>Biological Assay - methods</subject><subject>Cell Line, Tumor</subject><subject>Comet assay</subject><subject>DNA Damage</subject><subject>Ether - chemistry</subject><subject>Flavonoids</subject><subject>Health aspects</subject><subject>HepG2</subject><subject>Hops</subject><subject>Humans</subject><subject>Humulus - chemistry</subject><subject>Humulus lupulus</subject><subject>Microsomes</subject><subject>Mutagens - pharmacology</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Propiophenones - isolation & purification</subject><subject>Propiophenones - pharmacology</subject><subject>Quinolines - toxicity</subject><subject>Rats</subject><subject>Salmonella - chemistry</subject><subject>Salmonella/microsomal assay</subject><subject>Xanthohumol</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV2L1DAUhoO4uOPqPxAJeKMX7Z5sk6bxQhgWdRcG9kbBu5CP050MbVOaVpx_b8YugjBIICHJ856E8xDyhkHJgNXXh3LcH3v05Q2ALEGVAM0zsmE1awpQ4sdzsgHFeSEZqy7Jy5QOAIwrCS_IJZNKqKqWG9Jthzn0y2wecQguzEcaW7qPY6Lv6d3SL92SaLeMf9Zd-eEjtSGalMyx8GFCN6On7WTcHOJgThM1g6chxW7d5WK_zDDv437pY_eKXLSmS_j6ab0i3798_nZ7V-wevt7fbneF47WYCyVRSV8zY5TwljvLJDIreKUsoIXGQs1FaxsnlOHcOyftDZNSNGCrVvGmuiLv1rqPpkMdhjbO-Y99SE5vM6ig4QIyVZyhch9wMl0csA35-B--PMPn4bEP7myArwE3xZQmbPU4hd5MR81Anxzqg14d6pNDDUpnhzn2do2Niz3d_Q09ScvApxXA3MOfASedXMDB4apE-xj-_8JvigavaA</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Kac, Javor</creator><creator>Plazar, Janja</creator><creator>Mlinarič, Aleš</creator><creator>Žegura, Bojana</creator><creator>Lah, Tamara T.</creator><creator>Filipič, Metka</creator><general>Elsevier GmbH</general><general>Urban & Fischer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20080301</creationdate><title>Antimutagenicity of hops ( Humulus lupulus L.): bioassay-directed fractionation and isolation of xanthohumol</title><author>Kac, Javor ; Plazar, Janja ; Mlinarič, Aleš ; Žegura, Bojana ; Lah, Tamara T. ; Filipič, Metka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-97e97d61aa95db4cb17e1b5439b0eb08b0645fb8c59a44dcc7b2177580b3f9483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>4-Nitroquinoline-1-oxide - toxicity</topic><topic>Animals</topic><topic>Antimutagen</topic><topic>Antimutagenic Agents - chemistry</topic><topic>Antimutagenic Agents - isolation & purification</topic><topic>Antimutagenic Agents - pharmacology</topic><topic>Antioxidants</topic><topic>Biological assay</topic><topic>Biological Assay - methods</topic><topic>Cell Line, Tumor</topic><topic>Comet assay</topic><topic>DNA Damage</topic><topic>Ether - chemistry</topic><topic>Flavonoids</topic><topic>Health aspects</topic><topic>HepG2</topic><topic>Hops</topic><topic>Humans</topic><topic>Humulus - chemistry</topic><topic>Humulus lupulus</topic><topic>Microsomes</topic><topic>Mutagens - pharmacology</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Propiophenones - isolation & purification</topic><topic>Propiophenones - pharmacology</topic><topic>Quinolines - toxicity</topic><topic>Rats</topic><topic>Salmonella - chemistry</topic><topic>Salmonella/microsomal assay</topic><topic>Xanthohumol</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kac, Javor</creatorcontrib><creatorcontrib>Plazar, Janja</creatorcontrib><creatorcontrib>Mlinarič, Aleš</creatorcontrib><creatorcontrib>Žegura, Bojana</creatorcontrib><creatorcontrib>Lah, Tamara T.</creatorcontrib><creatorcontrib>Filipič, Metka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kac, Javor</au><au>Plazar, Janja</au><au>Mlinarič, Aleš</au><au>Žegura, Bojana</au><au>Lah, Tamara T.</au><au>Filipič, Metka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimutagenicity of hops ( Humulus lupulus L.): bioassay-directed fractionation and isolation of xanthohumol</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>15</volume><issue>3</issue><spage>216</spage><epage>220</epage><pages>216-220</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Bioassay-directed fractionation with a
Salmonella/microsomal assay against the food borne mutagen 2-amino-3-methylimidazo[4,5-
f]quinoline (IQ) was used to identify antimutagenic components of hops. Hops pellets extracted with diethylether showed antimutagenic activity against mutations induced by IQ. Fractionation of the diethylether extract (DE) by column chromatography, followed by semi-preparative HPLC yielded two fractions (E4b and E4d) with strong antimutagenic activity against IQ induced mutations. Separation of fraction E4b resulted in inactive fractions, while fraction E4d has been identified to be xanthohumol. In mammalian test system with human hepatoma HepG2 cells fraction E4d at 10
μg/ml completely prevented formation of IQ induced DNA damage. These results indicate that xanthohumol is a very promising potential protective agent against genotoxicity of food borne carcinogens, which warrants further investigation.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>17959367</pmid><doi>10.1016/j.phymed.2007.09.008</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | 4-Nitroquinoline-1-oxide - toxicity Animals Antimutagen Antimutagenic Agents - chemistry Antimutagenic Agents - isolation & purification Antimutagenic Agents - pharmacology Antioxidants Biological assay Biological Assay - methods Cell Line, Tumor Comet assay DNA Damage Ether - chemistry Flavonoids Health aspects HepG2 Hops Humans Humulus - chemistry Humulus lupulus Microsomes Mutagens - pharmacology Plant Extracts - isolation & purification Plant Extracts - pharmacology Propiophenones - isolation & purification Propiophenones - pharmacology Quinolines - toxicity Rats Salmonella - chemistry Salmonella/microsomal assay Xanthohumol |
| title | Antimutagenicity of hops ( Humulus lupulus L.): bioassay-directed fractionation and isolation of xanthohumol |
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