Next-Generation-Sequencing-Based Simple Sequence Repeat

Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diver...

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Veröffentlicht in:Life (Basel, Switzerland) Switzerland), 2023-07, Vol.13 (7)
Hauptverfasser: Topu, Mustafa, Sesiz, Uğur, Bektaş, Harun, Toklu, Faruk, Özkan, Hakan
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container_issue 7
container_start_page
container_title Life (Basel, Switzerland)
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creator Topu, Mustafa
Sesiz, Uğur
Bektaş, Harun
Toklu, Faruk
Özkan, Hakan
description Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diversity in marker-assisted selection is still limited. In this study, novel SSR markers needed in lentil were identified using a next-generation sequencing approach. In this study, we created a ready-to-use SSR library for genetic diversity studies and breeding and evaluated the effectiveness of the obtained SSR markers in a recombinant inbred line (RIL) population.
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subjects Analysis
Beans
Biological diversity
DNA sequencing
Genetic aspects
Genetic research
Genomics
Legumes
Lentils
Methods
Microsatellites (Genetics)
Mimosaceae
Nucleotide sequencing
title Next-Generation-Sequencing-Based Simple Sequence Repeat
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