Next-Generation-Sequencing-Based Simple Sequence Repeat
Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diver...
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Veröffentlicht in: | Life (Basel, Switzerland) Switzerland), 2023-07, Vol.13 (7) |
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creator | Topu, Mustafa Sesiz, Uğur Bektaş, Harun Toklu, Faruk Özkan, Hakan |
description | Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diversity in marker-assisted selection is still limited. In this study, novel SSR markers needed in lentil were identified using a next-generation sequencing approach. In this study, we created a ready-to-use SSR library for genetic diversity studies and breeding and evaluated the effectiveness of the obtained SSR markers in a recombinant inbred line (RIL) population. |
doi_str_mv | 10.3390/life13071579 |
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Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diversity in marker-assisted selection is still limited. In this study, novel SSR markers needed in lentil were identified using a next-generation sequencing approach. In this study, we created a ready-to-use SSR library for genetic diversity studies and breeding and evaluated the effectiveness of the obtained SSR markers in a recombinant inbred line (RIL) population.</description><identifier>ISSN: 2075-1729</identifier><identifier>EISSN: 2075-1729</identifier><identifier>DOI: 10.3390/life13071579</identifier><language>eng</language><publisher>MDPI AG</publisher><subject>Analysis ; Beans ; Biological diversity ; DNA sequencing ; Genetic aspects ; Genetic research ; Genomics ; Legumes ; Lentils ; Methods ; Microsatellites (Genetics) ; Mimosaceae ; Nucleotide sequencing</subject><ispartof>Life (Basel, Switzerland), 2023-07, Vol.13 (7)</ispartof><rights>COPYRIGHT 2023 MDPI AG</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids></links><search><creatorcontrib>Topu, Mustafa</creatorcontrib><creatorcontrib>Sesiz, Uğur</creatorcontrib><creatorcontrib>Bektaş, Harun</creatorcontrib><creatorcontrib>Toklu, Faruk</creatorcontrib><creatorcontrib>Özkan, Hakan</creatorcontrib><title>Next-Generation-Sequencing-Based Simple Sequence Repeat</title><title>Life (Basel, Switzerland)</title><description>Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. 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In this study, we created a ready-to-use SSR library for genetic diversity studies and breeding and evaluated the effectiveness of the obtained SSR markers in a recombinant inbred line (RIL) population.</description><subject>Analysis</subject><subject>Beans</subject><subject>Biological diversity</subject><subject>DNA sequencing</subject><subject>Genetic aspects</subject><subject>Genetic research</subject><subject>Genomics</subject><subject>Legumes</subject><subject>Lentils</subject><subject>Methods</subject><subject>Microsatellites (Genetics)</subject><subject>Mimosaceae</subject><subject>Nucleotide sequencing</subject><issn>2075-1729</issn><issn>2075-1729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpVT0FqwzAQFKWFhjS3PsDXHpRqLcnyHtPQpoHQQpx7kK2VUXHkNHagz6-gOaQ7LDMMMwvL2COIuZQonrvgCaQwoA3esEkujOZgcry90vdsNgxfIk2hoSjVhJkP-hn5iiKd7Bj6yCv6PlNsQmz5ix3IZVU4HDvKLj5lWzqSHR_YnbfdQLMLT9nu7XW3fOebz9V6udjw1kjggN44UlJIdNjIWkKtHGhd5Mob5dBTYSl3tVGUFqV1KEQJoEsgr1DLKZv_nW1tR_sQfT-ebJPg6BCaPpIPyV8YjVAiCEiFp3-FlBnTh609D8N-XW2vs79EWlk-</recordid><startdate>20230701</startdate><enddate>20230701</enddate><creator>Topu, Mustafa</creator><creator>Sesiz, Uğur</creator><creator>Bektaş, Harun</creator><creator>Toklu, Faruk</creator><creator>Özkan, Hakan</creator><general>MDPI AG</general><scope>ISR</scope></search><sort><creationdate>20230701</creationdate><title>Next-Generation-Sequencing-Based Simple Sequence Repeat</title><author>Topu, Mustafa ; Sesiz, Uğur ; Bektaş, Harun ; Toklu, Faruk ; Özkan, Hakan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g731-19f7de43039d9c3b31b4d155624f74d9fe6ae2db74eb7493ad900811581ef4953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Analysis</topic><topic>Beans</topic><topic>Biological diversity</topic><topic>DNA sequencing</topic><topic>Genetic aspects</topic><topic>Genetic research</topic><topic>Genomics</topic><topic>Legumes</topic><topic>Lentils</topic><topic>Methods</topic><topic>Microsatellites (Genetics)</topic><topic>Mimosaceae</topic><topic>Nucleotide sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Topu, Mustafa</creatorcontrib><creatorcontrib>Sesiz, Uğur</creatorcontrib><creatorcontrib>Bektaş, Harun</creatorcontrib><creatorcontrib>Toklu, Faruk</creatorcontrib><creatorcontrib>Özkan, Hakan</creatorcontrib><collection>Gale In Context: Science</collection><jtitle>Life (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Topu, Mustafa</au><au>Sesiz, Uğur</au><au>Bektaş, Harun</au><au>Toklu, Faruk</au><au>Özkan, Hakan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Next-Generation-Sequencing-Based Simple Sequence Repeat</atitle><jtitle>Life (Basel, Switzerland)</jtitle><date>2023-07-01</date><risdate>2023</risdate><volume>13</volume><issue>7</issue><issn>2075-1729</issn><eissn>2075-1729</eissn><abstract>Although lentil is not as popular as other legumes, it is a climate-resilient legume crop because of its high protein content, nitrogen fixation, and abiotic stress tolerance ability. Even though it can be grown on almost every continent and is distributed globally, the use of existing genetic diversity in marker-assisted selection is still limited. In this study, novel SSR markers needed in lentil were identified using a next-generation sequencing approach. In this study, we created a ready-to-use SSR library for genetic diversity studies and breeding and evaluated the effectiveness of the obtained SSR markers in a recombinant inbred line (RIL) population.</abstract><pub>MDPI AG</pub><doi>10.3390/life13071579</doi></addata></record> |
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source | MDPI - Multidisciplinary Digital Publishing Institute; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
subjects | Analysis Beans Biological diversity DNA sequencing Genetic aspects Genetic research Genomics Legumes Lentils Methods Microsatellites (Genetics) Mimosaceae Nucleotide sequencing |
title | Next-Generation-Sequencing-Based Simple Sequence Repeat |
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