Mercuric Ion Stabilizes Levansucrase Secreted by Acetobacter nitrogenifigens Strain RG1.sup.T

The purification and characterization of an extracellular levansucrase enzyme produced by novel nitrogen-fixer Acetobacter nitrogenifigens strain RG1.sup.T is described. Culture conditions were optimized for maximum levansucrase production. Levansucrase purified to homogeneity by tenfold purificatio...

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Veröffentlicht in:The protein journal 2011-04, Vol.30 (4), p.262
Hauptverfasser: Paul, Arundhati, Samaddar, Neeloy, Dutta, Debasree, Bagchi, Abhishek, Chakravorty, Somnath, Chakraborty, Writachit, Gachhui, Ratan
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container_issue 4
container_start_page 262
container_title The protein journal
container_volume 30
creator Paul, Arundhati
Samaddar, Neeloy
Dutta, Debasree
Bagchi, Abhishek
Chakravorty, Somnath
Chakraborty, Writachit
Gachhui, Ratan
description The purification and characterization of an extracellular levansucrase enzyme produced by novel nitrogen-fixer Acetobacter nitrogenifigens strain RG1.sup.T is described. Culture conditions were optimized for maximum levansucrase production. Levansucrase purified to homogeneity by tenfold purification has a molecular weight of 65 kDa, contained four cysteine residues, polymerized raffinose and was stable for 21 days at pH 6.0 when stored at 4 °C or -20 °C but was vulnerable to DTT and [beta]-mercaptoethanol. Interestingly, this enzyme showed enhanced hydrolytic and polymerization activity in the presence of mercuric ion which, to our knowledge, is the first report for any levansucrase enzyme characterized so far. Evidences obtained from Native PAGE, tryptophan fluorescence study and activity measurements at different temperatures and in the presence of thiol modifying agents, show that mercuric ion stabilizes the enzyme. Levan, synthesized by the enzyme, has a molecular weight of 7,080 kDa and was shown to be a homopolymer of fructose.
doi_str_mv 10.1007/s10930-011-9328-y
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subjects Cysteine
Enzymes
Fructose
Polymerization
Thiols
title Mercuric Ion Stabilizes Levansucrase Secreted by Acetobacter nitrogenifigens Strain RG1.sup.T
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