Quantitative Determination of 2-Methoxypancracine in Hippeastrum reticulatum Bulbs by HPLC-DAD

2-Methoxypancracine is a montanine-type alkaloid exhibiting anti-inflammatory, antioxidant, and antibacterial effects. The bulbs of Hippeastrum reticulatum (L’Hér.) Herb. contain large amounts of 2-methoxypancracine. The aim of this study was to develop a HPLC-DAD method to quantify 2-methoxypancrac...

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Veröffentlicht in:Pharmaceutical chemistry journal 2022-07, Vol.56 (4), p.487-492
Hauptverfasser: Hoang, Trang Huyen Xuan, Nguyen, Tien Huu, Huong, Phung Thanh, Raal, Ain, Nguyen, Hoai Thi
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container_start_page 487
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Nguyen, Tien Huu
Huong, Phung Thanh
Raal, Ain
Nguyen, Hoai Thi
description 2-Methoxypancracine is a montanine-type alkaloid exhibiting anti-inflammatory, antioxidant, and antibacterial effects. The bulbs of Hippeastrum reticulatum (L’Hér.) Herb. contain large amounts of 2-methoxypancracine. The aim of this study was to develop a HPLC-DAD method to quantify 2-methoxypancracine content in H. reticulatum. The HPLC analysis was carried out using a Poroshell 120 EC-C18 column eluted with acetonitrile and 0.01% methylamine (35:65, v/v) mixture in water at a flow rate of 0.5 mL/min with detection at 205 nm. Method validation was performed to assure its linearity, precision, accuracy, as well as the limit of detection (LOD) and limit of quantitation (LOQ). 2-Methoxypancracine displayed a linear relationship within a range of 2.5 – 103 μg/mL. The developed method was shown to be precise with relative standard deviation less than 2.5%. The average recovery of 2-methoxypancracine was 101.2. The LOD and LOQ values were calculated as 0.08 μg/mL and 0.25 μg/mL, respectively. The concentrations of 2-methoxypancracine in the alkaloid fraction and in the dried powder of H. reticulatum were 28.95%, and 0.0740%, respectively. The validated method provides a useful tool for the quantification of 2-methoxypancracine in H. reticulatum . This is the first report of significant 2-methoxypancracine content in H. reticulatum .
doi_str_mv 10.1007/s11094-022-02666-3
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The bulbs of Hippeastrum reticulatum (L’Hér.) Herb. contain large amounts of 2-methoxypancracine. The aim of this study was to develop a HPLC-DAD method to quantify 2-methoxypancracine content in H. reticulatum. The HPLC analysis was carried out using a Poroshell 120 EC-C18 column eluted with acetonitrile and 0.01% methylamine (35:65, v/v) mixture in water at a flow rate of 0.5 mL/min with detection at 205 nm. Method validation was performed to assure its linearity, precision, accuracy, as well as the limit of detection (LOD) and limit of quantitation (LOQ). 2-Methoxypancracine displayed a linear relationship within a range of 2.5 – 103 μg/mL. The developed method was shown to be precise with relative standard deviation less than 2.5%. The average recovery of 2-methoxypancracine was 101.2. The LOD and LOQ values were calculated as 0.08 μg/mL and 0.25 μg/mL, respectively. 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subjects Acetonitrile
Analysis
High performance liquid chromatography
Medicine
Organic Chemistry
Pharmacology/Toxicology
Pharmacy
title Quantitative Determination of 2-Methoxypancracine in Hippeastrum reticulatum Bulbs by HPLC-DAD
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