Isolation of Nucleoid Fraction from Mycoplasma gallisepticum Cells with Synchronized Cell Division
It is assumed that unknown mechanisms can be involved in adaptation Mycoplasma gallisepticum to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of M. gallisepticum with synchro...
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| Veröffentlicht in: | Bulletin of experimental biology and medicine 2021-10, Vol.171 (6), p.760-763 |
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| container_title | Bulletin of experimental biology and medicine |
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| creator | Zubov, A. I. Ladygina, V. G. Galyamina, M. A. Pobeguts, O. V. Fisunov, G. Yu |
| description | It is assumed that unknown mechanisms can be involved in adaptation
Mycoplasma gallisepticum
to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of
M. gallisepticum
with synchronized division and isolated the nucleoid fraction from this culture by the method of mild cell lysis and centrifugation in a sucrose gradient. Liquid chromatography-mass spectrometry analysis of the proteome showed that in comparison with the cell lysate, the nucleoid fraction was enriched with DNA-binding proteins. This analysis will help to find new nucleoid-associated proteins and to study their dynamics, distribution, and their role during infection and under stress conditions. |
| doi_str_mv | 10.1007/s10517-021-05311-3 |
| format | Article |
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Mycoplasma gallisepticum
to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of
M. gallisepticum
with synchronized division and isolated the nucleoid fraction from this culture by the method of mild cell lysis and centrifugation in a sucrose gradient. Liquid chromatography-mass spectrometry analysis of the proteome showed that in comparison with the cell lysate, the nucleoid fraction was enriched with DNA-binding proteins. This analysis will help to find new nucleoid-associated proteins and to study their dynamics, distribution, and their role during infection and under stress conditions.</description><identifier>ISSN: 0007-4888</identifier><identifier>EISSN: 1573-8221</identifier><identifier>DOI: 10.1007/s10517-021-05311-3</identifier><identifier>PMID: 34705179</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject><![CDATA[Bacterial Proteins - classification ; Bacterial Proteins - genetics ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Binding proteins ; Biomedical and Life Sciences ; Biomedicine ; Cell Biology ; Cell culture ; Cell Division ; Centrifugation ; Centrifugation, Density Gradient - methods ; Chromatography, Liquid ; Chromosomes ; Culture Media - chemistry ; DNA, Bacterial - genetics ; DNA-binding protein ; DNA-Binding Proteins - classification ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - isolation & purification ; DNA-Binding Proteins - metabolism ; Ethylenediaminetetraacetic acid ; Gene Expression ; Internal Medicine ; Laboratory Medicine ; Life Sciences & Biomedicine ; Liquid chromatography ; Lysis ; Mass Spectrometry ; Mass spectroscopy ; Medicine, Research & Experimental ; Mycoplasma gallisepticum ; Mycoplasma gallisepticum - genetics ; Mycoplasma gallisepticum - metabolism ; Nuclear Proteins - classification ; Nuclear Proteins - genetics ; Nuclear Proteins - isolation & purification ; Nuclear Proteins - metabolism ; Pathology ; Protein binding ; Proteome - classification ; Proteome - genetics ; Proteome - isolation & purification ; Proteome - metabolism ; Proteomes ; Research & Experimental Medicine ; Science & Technology ; Sucrose]]></subject><ispartof>Bulletin of experimental biology and medicine, 2021-10, Vol.171 (6), p.760-763</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021</rights><rights>2021. Springer Science+Business Media, LLC, part of Springer Nature.</rights><rights>COPYRIGHT 2021 Springer</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>0</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000712501100003</woscitedreferencesoriginalsourcerecordid><cites>FETCH-LOGICAL-c424t-54fc5ed79e4e5551f09f91036890d17960f2f37c88b7080bf7609e4a951df8eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10517-021-05311-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10517-021-05311-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27933,27934,39267,41497,42566,51328</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34705179$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zubov, A. I.</creatorcontrib><creatorcontrib>Ladygina, V. G.</creatorcontrib><creatorcontrib>Galyamina, M. A.</creatorcontrib><creatorcontrib>Pobeguts, O. V.</creatorcontrib><creatorcontrib>Fisunov, G. Yu</creatorcontrib><title>Isolation of Nucleoid Fraction from Mycoplasma gallisepticum Cells with Synchronized Cell Division</title><title>Bulletin of experimental biology and medicine</title><addtitle>Bull Exp Biol Med</addtitle><addtitle>B EXP BIOL MED</addtitle><addtitle>Bull Exp Biol Med</addtitle><description>It is assumed that unknown mechanisms can be involved in adaptation
Mycoplasma gallisepticum
to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of
M. gallisepticum
with synchronized division and isolated the nucleoid fraction from this culture by the method of mild cell lysis and centrifugation in a sucrose gradient. Liquid chromatography-mass spectrometry analysis of the proteome showed that in comparison with the cell lysate, the nucleoid fraction was enriched with DNA-binding proteins. This analysis will help to find new nucleoid-associated proteins and to study their dynamics, distribution, and their role during infection and under stress conditions.</description><subject>Bacterial Proteins - classification</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Binding proteins</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Biology</subject><subject>Cell culture</subject><subject>Cell Division</subject><subject>Centrifugation</subject><subject>Centrifugation, Density Gradient - methods</subject><subject>Chromatography, Liquid</subject><subject>Chromosomes</subject><subject>Culture Media - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA-binding protein</subject><subject>DNA-Binding Proteins - classification</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - isolation & purification</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Ethylenediaminetetraacetic acid</subject><subject>Gene Expression</subject><subject>Internal Medicine</subject><subject>Laboratory Medicine</subject><subject>Life Sciences & Biomedicine</subject><subject>Liquid chromatography</subject><subject>Lysis</subject><subject>Mass Spectrometry</subject><subject>Mass spectroscopy</subject><subject>Medicine, Research & Experimental</subject><subject>Mycoplasma gallisepticum</subject><subject>Mycoplasma gallisepticum - genetics</subject><subject>Mycoplasma gallisepticum - metabolism</subject><subject>Nuclear Proteins - classification</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - isolation & purification</subject><subject>Nuclear Proteins - metabolism</subject><subject>Pathology</subject><subject>Protein binding</subject><subject>Proteome - classification</subject><subject>Proteome - genetics</subject><subject>Proteome - isolation & purification</subject><subject>Proteome - metabolism</subject><subject>Proteomes</subject><subject>Research & Experimental Medicine</subject><subject>Science & Technology</subject><subject>Sucrose</subject><issn>0007-4888</issn><issn>1573-8221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNktFr1TAUxoso7jr9B3yQgiAD6TxpmiZ9HHXTwdQH9Tm06cluRttck9Zx_es9vXduTkQkDyGH33f4zsmXJM8ZHDMA-SYyEExmkLMMBGcs4w-SFROSZyrP2cNkBURlhVLqIHkS49XyhJI9Tg54IRdptUra8-j7ZnJ-TL1NP86mR--69Cw0Zle0wQ_ph63xm76JQ5NeNn3vIm4mZ-YhrbHvY3rtpnX6eTuadfCj-4Hdrp6-dd9dpB5Pk0e26SM-u7kPk69np1_q99nFp3fn9clFZoq8mDJRWCOwkxUWKIRgFipbMeClqqAjryXY3HJplGolKGitLIHYphKsswpbfpgc7ftugv82Y5z04KIhJ82Ifo46F6qsqrwsFKEv_0Cv_BxGckdUBVJKyMs7ioZG7UbrJ1rL0lSflFUhVMFLRtTxXyg6HQ7O-BGto_o9wavfBGts-mlNnzAv-473wXwPmuBjDGj1JrihCVvNQC8J0PsEaEqA3iVAcxK9uBltbgfsbiW_vpwAtQeusfU2GoejwVtsyQjLBTDqD8BrN-3CUft5nEj6-v-lRPM9HYkYLzHcLfkf_n8Cy97ZVg</recordid><startdate>20211001</startdate><enddate>20211001</enddate><creator>Zubov, A. 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Yu</creator><general>Springer US</general><general>Springer Nature</general><general>Springer</general><general>Springer Nature B.V</general><scope>BLEPL</scope><scope>DTL</scope><scope>HGBXW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20211001</creationdate><title>Isolation of Nucleoid Fraction from Mycoplasma gallisepticum Cells with Synchronized Cell Division</title><author>Zubov, A. 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I.</creatorcontrib><creatorcontrib>Ladygina, V. G.</creatorcontrib><creatorcontrib>Galyamina, M. A.</creatorcontrib><creatorcontrib>Pobeguts, O. V.</creatorcontrib><creatorcontrib>Fisunov, G. 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Mycoplasma gallisepticum
to unfavorable factors, one of these can be local rearrangements of the structure and spatial organization of the chromosome. To study these mechanisms, we obtained a culture of
M. gallisepticum
with synchronized division and isolated the nucleoid fraction from this culture by the method of mild cell lysis and centrifugation in a sucrose gradient. Liquid chromatography-mass spectrometry analysis of the proteome showed that in comparison with the cell lysate, the nucleoid fraction was enriched with DNA-binding proteins. This analysis will help to find new nucleoid-associated proteins and to study their dynamics, distribution, and their role during infection and under stress conditions.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>34705179</pmid><doi>10.1007/s10517-021-05311-3</doi><tpages>4</tpages></addata></record> |
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| subjects | Bacterial Proteins - classification Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Binding proteins Biomedical and Life Sciences Biomedicine Cell Biology Cell culture Cell Division Centrifugation Centrifugation, Density Gradient - methods Chromatography, Liquid Chromosomes Culture Media - chemistry DNA, Bacterial - genetics DNA-binding protein DNA-Binding Proteins - classification DNA-Binding Proteins - genetics DNA-Binding Proteins - isolation & purification DNA-Binding Proteins - metabolism Ethylenediaminetetraacetic acid Gene Expression Internal Medicine Laboratory Medicine Life Sciences & Biomedicine Liquid chromatography Lysis Mass Spectrometry Mass spectroscopy Medicine, Research & Experimental Mycoplasma gallisepticum Mycoplasma gallisepticum - genetics Mycoplasma gallisepticum - metabolism Nuclear Proteins - classification Nuclear Proteins - genetics Nuclear Proteins - isolation & purification Nuclear Proteins - metabolism Pathology Protein binding Proteome - classification Proteome - genetics Proteome - isolation & purification Proteome - metabolism Proteomes Research & Experimental Medicine Science & Technology Sucrose |
| title | Isolation of Nucleoid Fraction from Mycoplasma gallisepticum Cells with Synchronized Cell Division |
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