Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163
Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this s...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2021-04, Vol.105 (7), p.2713-2723 |
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| creator | Zhao, Deyin Meng, Fanqiang Zhou, Libang Lu, Fengxia Bie, Xiaomei Sun, Jing Lu, Zhaoxin Lu, Yingjian |
| description | Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this study, the effect of maltose on plantaricin EF production and its regulation mechanism in
Lactobacillus plantarum
163 were investigated. Maltose significantly improved the biomass and plantaricin EF production, which increased by 3.35 and 3.99 times comparing to the control without maltose, respectively. The maximum production of plantaricin E and F in fed-batch fermentation were 10.55 mg/L and 22.94 mg/L, respectively. Besides, qPCR results showed that maltose remarkably improved transcription of
plnA
,
plnB
,
plnD
,
plnE
,
plnF, plnG1
and
plnH
, and heighten transcription of
lamR
,
lamK
,
hpk6
and
rrp6
. These results provided an effective method to enhance plantaricin EF production and revealed a possible regulatory mechanism from transcriptome results that
hpk6
,
rrp6
,
lamK
and
lamR
were relative to plantaricin EF production. Genes,
hpk6
and
rrp6
, promote transcription of
plnG1
, whereas
lamK
and
lamR
enhance transcription of
plnA
,
plnB
and
plnD
, which increased plantaricin EF production.
Keypoints
• Maltose was proved to be effective in promoting the biosynthesis of plantaricin EF.
• Maltose promoted the transcription of biosynthesis and secretion genes of plantaricin EF.
• Up-regulation of genes lamR, lamK, hpk6 and rrp6 heightened the plantaricin EF production.
Graphical abstract |
| doi_str_mv | 10.1007/s00253-021-11218-w |
| format | Article |
| fullrecord | <record><control><sourceid>gale_cross</sourceid><recordid>TN_cdi_gale_infotracacademiconefile_A656768753</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A656768753</galeid><sourcerecordid>A656768753</sourcerecordid><originalsourceid>FETCH-LOGICAL-c513t-962bc4c2cad68cd7a8555c0e211b5f89e2b9c5a1f7adc9a84204e927cda6c63a3</originalsourceid><addsrcrecordid>eNqNktuKFDEQhoMo7jj6Al5IwJsV6TWHTjp9uQy7ujAieLgO6XT1mKU7GTvpHeftzdh7YEREAimo-v6iqvgReknJGSWkehcJYYIXhNGCUkZVsXuEFrTkrCCSlo_RgtBKFJWo1Ql6FuM1IZQpKZ-iE84rSrioFih-NH0KETB0HdjkbgC7YTuGG-c3OMd2ysngsfEtHmEz9SaFcY8bF-Lep-8QXcShw9ve-GRGZ53HF5c4_2tjU2iMdX0_xbv6NGAq-XP0pDN9hBe3cYm-XV58XX0o1p_eX63O14UVlKeilqyxpWXWtFLZtjJKCGEJMEob0akaWFNbYWhXmdbWRpWMlFCzyrZGWskNX6LTuW_e48cEMenBRQt9ngXCFDUT-SCiJERk9PUf6HWYRp-nO1BSCs4JfaA2pgftfBfSaOyhqT6XQlZSVRlcorO_UPm1MDgbPHQu548Eb44EmUnwM23MFKO--vL5mGUza8cQ4wid3o5uMONeU6IPrtCzK3R2hf7tCr3Lole3203NAO295M4GGVAzsIMmdNE68BbuMUKIZIookk-VPbRyyRw8sQqTT1n69v-lmeYzHTPhNzA-3Pkf8_8CZq3ilg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2506653301</pqid></control><display><type>article</type><title>Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163</title><source>MEDLINE</source><source>SpringerNature Journals</source><source>Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /></source><creator>Zhao, Deyin ; Meng, Fanqiang ; Zhou, Libang ; Lu, Fengxia ; Bie, Xiaomei ; Sun, Jing ; Lu, Zhaoxin ; Lu, Yingjian</creator><creatorcontrib>Zhao, Deyin ; Meng, Fanqiang ; Zhou, Libang ; Lu, Fengxia ; Bie, Xiaomei ; Sun, Jing ; Lu, Zhaoxin ; Lu, Yingjian</creatorcontrib><description>Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this study, the effect of maltose on plantaricin EF production and its regulation mechanism in
Lactobacillus plantarum
163 were investigated. Maltose significantly improved the biomass and plantaricin EF production, which increased by 3.35 and 3.99 times comparing to the control without maltose, respectively. The maximum production of plantaricin E and F in fed-batch fermentation were 10.55 mg/L and 22.94 mg/L, respectively. Besides, qPCR results showed that maltose remarkably improved transcription of
plnA
,
plnB
,
plnD
,
plnE
,
plnF, plnG1
and
plnH
, and heighten transcription of
lamR
,
lamK
,
hpk6
and
rrp6
. These results provided an effective method to enhance plantaricin EF production and revealed a possible regulatory mechanism from transcriptome results that
hpk6
,
rrp6
,
lamK
and
lamR
were relative to plantaricin EF production. Genes,
hpk6
and
rrp6
, promote transcription of
plnG1
, whereas
lamK
and
lamR
enhance transcription of
plnA
,
plnB
and
plnD
, which increased plantaricin EF production.
Keypoints
• Maltose was proved to be effective in promoting the biosynthesis of plantaricin EF.
• Maltose promoted the transcription of biosynthesis and secretion genes of plantaricin EF.
• Up-regulation of genes lamR, lamK, hpk6 and rrp6 heightened the plantaricin EF production.
Graphical abstract</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-021-11218-w</identifier><identifier>PMID: 33710357</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antibacterial agents ; Antiinfectives and antibacterials ; Bacteriocins ; Bacteriocins - genetics ; Bacteriocins - metabolism ; Batch culture ; Biomedical and Life Sciences ; Biosynthesis ; Biotechnological Products and Process Engineering ; Biotechnology ; Biotechnology & Applied Microbiology ; Biotechnology industry ; Chemical properties ; Fermentation ; Food preservation ; Gene expression ; Gene regulation ; Genes ; Identification and classification ; Lactobacilli ; Lactobacillus plantarum ; Lactobacillus plantarum - genetics ; Lactobacillus plantarum - metabolism ; Life Sciences ; Life Sciences & Biomedicine ; Maltose ; Microbial Genetics and Genomics ; Microbiology ; Microorganisms ; Physiological aspects ; Production management ; Production processes ; Regulatory mechanisms (biology) ; Science & Technology ; Spoilage ; Sugars ; Transcription ; Transcriptomes</subject><ispartof>Applied microbiology and biotechnology, 2021-04, Vol.105 (7), p.2713-2723</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021</rights><rights>COPYRIGHT 2021 Springer</rights><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>10</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000628080400001</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c513t-962bc4c2cad68cd7a8555c0e211b5f89e2b9c5a1f7adc9a84204e927cda6c63a3</citedby><cites>FETCH-LOGICAL-c513t-962bc4c2cad68cd7a8555c0e211b5f89e2b9c5a1f7adc9a84204e927cda6c63a3</cites><orcidid>0000-0001-9288-7419 ; 0000-0001-6330-4536</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-021-11218-w$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-021-11218-w$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,39263,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33710357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Deyin</creatorcontrib><creatorcontrib>Meng, Fanqiang</creatorcontrib><creatorcontrib>Zhou, Libang</creatorcontrib><creatorcontrib>Lu, Fengxia</creatorcontrib><creatorcontrib>Bie, Xiaomei</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Lu, Zhaoxin</creatorcontrib><creatorcontrib>Lu, Yingjian</creatorcontrib><title>Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>APPL MICROBIOL BIOT</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this study, the effect of maltose on plantaricin EF production and its regulation mechanism in
Lactobacillus plantarum
163 were investigated. Maltose significantly improved the biomass and plantaricin EF production, which increased by 3.35 and 3.99 times comparing to the control without maltose, respectively. The maximum production of plantaricin E and F in fed-batch fermentation were 10.55 mg/L and 22.94 mg/L, respectively. Besides, qPCR results showed that maltose remarkably improved transcription of
plnA
,
plnB
,
plnD
,
plnE
,
plnF, plnG1
and
plnH
, and heighten transcription of
lamR
,
lamK
,
hpk6
and
rrp6
. These results provided an effective method to enhance plantaricin EF production and revealed a possible regulatory mechanism from transcriptome results that
hpk6
,
rrp6
,
lamK
and
lamR
were relative to plantaricin EF production. Genes,
hpk6
and
rrp6
, promote transcription of
plnG1
, whereas
lamK
and
lamR
enhance transcription of
plnA
,
plnB
and
plnD
, which increased plantaricin EF production.
Keypoints
• Maltose was proved to be effective in promoting the biosynthesis of plantaricin EF.
• Maltose promoted the transcription of biosynthesis and secretion genes of plantaricin EF.
• Up-regulation of genes lamR, lamK, hpk6 and rrp6 heightened the plantaricin EF production.
Graphical abstract</description><subject>Antibacterial agents</subject><subject>Antiinfectives and antibacterials</subject><subject>Bacteriocins</subject><subject>Bacteriocins - genetics</subject><subject>Bacteriocins - metabolism</subject><subject>Batch culture</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Biotechnological Products and Process Engineering</subject><subject>Biotechnology</subject><subject>Biotechnology & Applied Microbiology</subject><subject>Biotechnology industry</subject><subject>Chemical properties</subject><subject>Fermentation</subject><subject>Food preservation</subject><subject>Gene expression</subject><subject>Gene regulation</subject><subject>Genes</subject><subject>Identification and classification</subject><subject>Lactobacilli</subject><subject>Lactobacillus plantarum</subject><subject>Lactobacillus plantarum - genetics</subject><subject>Lactobacillus plantarum - metabolism</subject><subject>Life Sciences</subject><subject>Life Sciences & Biomedicine</subject><subject>Maltose</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Physiological aspects</subject><subject>Production management</subject><subject>Production processes</subject><subject>Regulatory mechanisms (biology)</subject><subject>Science & Technology</subject><subject>Spoilage</subject><subject>Sugars</subject><subject>Transcription</subject><subject>Transcriptomes</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>HGBXW</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNktuKFDEQhoMo7jj6Al5IwJsV6TWHTjp9uQy7ujAieLgO6XT1mKU7GTvpHeftzdh7YEREAimo-v6iqvgReknJGSWkehcJYYIXhNGCUkZVsXuEFrTkrCCSlo_RgtBKFJWo1Ql6FuM1IZQpKZ-iE84rSrioFih-NH0KETB0HdjkbgC7YTuGG-c3OMd2ysngsfEtHmEz9SaFcY8bF-Lep-8QXcShw9ve-GRGZ53HF5c4_2tjU2iMdX0_xbv6NGAq-XP0pDN9hBe3cYm-XV58XX0o1p_eX63O14UVlKeilqyxpWXWtFLZtjJKCGEJMEob0akaWFNbYWhXmdbWRpWMlFCzyrZGWskNX6LTuW_e48cEMenBRQt9ngXCFDUT-SCiJERk9PUf6HWYRp-nO1BSCs4JfaA2pgftfBfSaOyhqT6XQlZSVRlcorO_UPm1MDgbPHQu548Eb44EmUnwM23MFKO--vL5mGUza8cQ4wid3o5uMONeU6IPrtCzK3R2hf7tCr3Lole3203NAO295M4GGVAzsIMmdNE68BbuMUKIZIookk-VPbRyyRw8sQqTT1n69v-lmeYzHTPhNzA-3Pkf8_8CZq3ilg</recordid><startdate>20210401</startdate><enddate>20210401</enddate><creator>Zhao, Deyin</creator><creator>Meng, Fanqiang</creator><creator>Zhou, Libang</creator><creator>Lu, Fengxia</creator><creator>Bie, Xiaomei</creator><creator>Sun, Jing</creator><creator>Lu, Zhaoxin</creator><creator>Lu, Yingjian</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature</general><general>Springer</general><general>Springer Nature 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effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163</title><author>Zhao, Deyin ; Meng, Fanqiang ; Zhou, Libang ; Lu, Fengxia ; Bie, Xiaomei ; Sun, Jing ; Lu, Zhaoxin ; Lu, Yingjian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c513t-962bc4c2cad68cd7a8555c0e211b5f89e2b9c5a1f7adc9a84204e927cda6c63a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Antibacterial agents</topic><topic>Antiinfectives and antibacterials</topic><topic>Bacteriocins</topic><topic>Bacteriocins - genetics</topic><topic>Bacteriocins - metabolism</topic><topic>Batch culture</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Biotechnological Products and Process Engineering</topic><topic>Biotechnology</topic><topic>Biotechnology & Applied Microbiology</topic><topic>Biotechnology industry</topic><topic>Chemical properties</topic><topic>Fermentation</topic><topic>Food preservation</topic><topic>Gene expression</topic><topic>Gene regulation</topic><topic>Genes</topic><topic>Identification and classification</topic><topic>Lactobacilli</topic><topic>Lactobacillus plantarum</topic><topic>Lactobacillus plantarum - genetics</topic><topic>Lactobacillus plantarum - metabolism</topic><topic>Life Sciences</topic><topic>Life Sciences & Biomedicine</topic><topic>Maltose</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Physiological aspects</topic><topic>Production management</topic><topic>Production processes</topic><topic>Regulatory mechanisms (biology)</topic><topic>Science & Technology</topic><topic>Spoilage</topic><topic>Sugars</topic><topic>Transcription</topic><topic>Transcriptomes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Deyin</creatorcontrib><creatorcontrib>Meng, 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biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Deyin</au><au>Meng, Fanqiang</au><au>Zhou, Libang</au><au>Lu, Fengxia</au><au>Bie, Xiaomei</au><au>Sun, Jing</au><au>Lu, Zhaoxin</au><au>Lu, Yingjian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><stitle>APPL MICROBIOL BIOT</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2021-04-01</date><risdate>2021</risdate><volume>105</volume><issue>7</issue><spage>2713</spage><epage>2723</epage><pages>2713-2723</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Plantaricin EF, a kind of natural antibacterial substance, has shown inhibitory effect on most pathogen and spoilage microorganisms, which possessed great potential in food preservation. However, the lower production of plantaricin EF has limited its large-scale production and application. In this study, the effect of maltose on plantaricin EF production and its regulation mechanism in
Lactobacillus plantarum
163 were investigated. Maltose significantly improved the biomass and plantaricin EF production, which increased by 3.35 and 3.99 times comparing to the control without maltose, respectively. The maximum production of plantaricin E and F in fed-batch fermentation were 10.55 mg/L and 22.94 mg/L, respectively. Besides, qPCR results showed that maltose remarkably improved transcription of
plnA
,
plnB
,
plnD
,
plnE
,
plnF, plnG1
and
plnH
, and heighten transcription of
lamR
,
lamK
,
hpk6
and
rrp6
. These results provided an effective method to enhance plantaricin EF production and revealed a possible regulatory mechanism from transcriptome results that
hpk6
,
rrp6
,
lamK
and
lamR
were relative to plantaricin EF production. Genes,
hpk6
and
rrp6
, promote transcription of
plnG1
, whereas
lamK
and
lamR
enhance transcription of
plnA
,
plnB
and
plnD
, which increased plantaricin EF production.
Keypoints
• Maltose was proved to be effective in promoting the biosynthesis of plantaricin EF.
• Maltose promoted the transcription of biosynthesis and secretion genes of plantaricin EF.
• Up-regulation of genes lamR, lamK, hpk6 and rrp6 heightened the plantaricin EF production.
Graphical abstract</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>33710357</pmid><doi>10.1007/s00253-021-11218-w</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0001-9288-7419</orcidid><orcidid>https://orcid.org/0000-0001-6330-4536</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0175-7598 |
| ispartof | Applied microbiology and biotechnology, 2021-04, Vol.105 (7), p.2713-2723 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_gale_infotracacademiconefile_A656768753 |
| source | MEDLINE; SpringerNature Journals; Web of Science - Science Citation Index Expanded - 2021<img src="https://exlibris-pub.s3.amazonaws.com/fromwos-v2.jpg" /> |
| subjects | Antibacterial agents Antiinfectives and antibacterials Bacteriocins Bacteriocins - genetics Bacteriocins - metabolism Batch culture Biomedical and Life Sciences Biosynthesis Biotechnological Products and Process Engineering Biotechnology Biotechnology & Applied Microbiology Biotechnology industry Chemical properties Fermentation Food preservation Gene expression Gene regulation Genes Identification and classification Lactobacilli Lactobacillus plantarum Lactobacillus plantarum - genetics Lactobacillus plantarum - metabolism Life Sciences Life Sciences & Biomedicine Maltose Microbial Genetics and Genomics Microbiology Microorganisms Physiological aspects Production management Production processes Regulatory mechanisms (biology) Science & Technology Spoilage Sugars Transcription Transcriptomes |
| title | Maltose effective improving production and regulatory biosynthesis of plantaricin EF in Lactobacillus plantarum 163 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-13T02%3A25%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Maltose%20effective%20improving%20production%20and%20regulatory%20biosynthesis%20of%20plantaricin%20EF%20in%20Lactobacillus%20plantarum%20163&rft.jtitle=Applied%20microbiology%20and%20biotechnology&rft.au=Zhao,%20Deyin&rft.date=2021-04-01&rft.volume=105&rft.issue=7&rft.spage=2713&rft.epage=2723&rft.pages=2713-2723&rft.issn=0175-7598&rft.eissn=1432-0614&rft_id=info:doi/10.1007/s00253-021-11218-w&rft_dat=%3Cgale_cross%3EA656768753%3C/gale_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2506653301&rft_id=info:pmid/33710357&rft_galeid=A656768753&rfr_iscdi=true |