Sperm kinematic subpopulations of the American crocodile
There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejacul...
Gespeichert in:
| Veröffentlicht in: | PLoS ONE 2021, Vol.16 (3), p.e0248270 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Report |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 3 |
| container_start_page | e0248270 |
| container_title | PLoS ONE |
| container_volume | 16 |
| creator | Valverde, Anthony Castro-Morales, Olivier Madrigal-Valverde, Mónica Camacho, Marlen Barquero, Vinicio Soler, Carles Roldan, Eduardo R. S |
| description | There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species. |
| doi_str_mv | 10.1371/journal.pone.0248270 |
| format | Report |
| fullrecord | <record><control><sourceid>gale</sourceid><recordid>TN_cdi_gale_infotracacademiconefile_A654395294</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A654395294</galeid><sourcerecordid>A654395294</sourcerecordid><originalsourceid>FETCH-gale_infotracacademiconefile_A6543952943</originalsourceid><addsrcrecordid>eNqVjr0OgjAYRRujifjzBg59AbC0UGAkRuOuO6nlqxZLS1p4fxkcXM0d7j3DSS5Ch5QkKSvSY-cmb4VJBmchITQraUEWKEorRmNOCVv-7DXahNARkrOS8wiVtwF8j9_aQi9GLXGYHoMbJjODswE7hccX4LoHr6WwWHonXasN7NBKCRNg_-0tSi7n--kaP4WBRlvlRi_knBZ6LedjapaamucZq3JaZexv4QPghEgl</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>report</recordtype></control><display><type>report</type><title>Sperm kinematic subpopulations of the American crocodile</title><source>Public Library of Science (PLoS) Journals Open Access</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Valverde, Anthony ; Castro-Morales, Olivier ; Madrigal-Valverde, Mónica ; Camacho, Marlen ; Barquero, Vinicio ; Soler, Carles ; Roldan, Eduardo R. S</creator><creatorcontrib>Valverde, Anthony ; Castro-Morales, Olivier ; Madrigal-Valverde, Mónica ; Camacho, Marlen ; Barquero, Vinicio ; Soler, Carles ; Roldan, Eduardo R. S</creatorcontrib><description>There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0248270</identifier><language>eng</language><publisher>Public Library of Science</publisher><subject>Distribution ; Environmental aspects ; Genetic aspects ; Identification and classification ; Kinematics ; Spermatozoa</subject><ispartof>PLoS ONE, 2021, Vol.16 (3), p.e0248270</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>776,780,860,4476,27902</link.rule.ids></links><search><creatorcontrib>Valverde, Anthony</creatorcontrib><creatorcontrib>Castro-Morales, Olivier</creatorcontrib><creatorcontrib>Madrigal-Valverde, Mónica</creatorcontrib><creatorcontrib>Camacho, Marlen</creatorcontrib><creatorcontrib>Barquero, Vinicio</creatorcontrib><creatorcontrib>Soler, Carles</creatorcontrib><creatorcontrib>Roldan, Eduardo R. S</creatorcontrib><title>Sperm kinematic subpopulations of the American crocodile</title><title>PLoS ONE</title><description>There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species.</description><subject>Distribution</subject><subject>Environmental aspects</subject><subject>Genetic aspects</subject><subject>Identification and classification</subject><subject>Kinematics</subject><subject>Spermatozoa</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>report</rsrctype><creationdate>2021</creationdate><recordtype>report</recordtype><sourceid/><recordid>eNqVjr0OgjAYRRujifjzBg59AbC0UGAkRuOuO6nlqxZLS1p4fxkcXM0d7j3DSS5Ch5QkKSvSY-cmb4VJBmchITQraUEWKEorRmNOCVv-7DXahNARkrOS8wiVtwF8j9_aQi9GLXGYHoMbJjODswE7hccX4LoHr6WwWHonXasN7NBKCRNg_-0tSi7n--kaP4WBRlvlRi_knBZ6LedjapaamucZq3JaZexv4QPghEgl</recordid><startdate>20210309</startdate><enddate>20210309</enddate><creator>Valverde, Anthony</creator><creator>Castro-Morales, Olivier</creator><creator>Madrigal-Valverde, Mónica</creator><creator>Camacho, Marlen</creator><creator>Barquero, Vinicio</creator><creator>Soler, Carles</creator><creator>Roldan, Eduardo R. S</creator><general>Public Library of Science</general><scope/></search><sort><creationdate>20210309</creationdate><title>Sperm kinematic subpopulations of the American crocodile</title><author>Valverde, Anthony ; Castro-Morales, Olivier ; Madrigal-Valverde, Mónica ; Camacho, Marlen ; Barquero, Vinicio ; Soler, Carles ; Roldan, Eduardo R. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-gale_infotracacademiconefile_A6543952943</frbrgroupid><rsrctype>reports</rsrctype><prefilter>reports</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Distribution</topic><topic>Environmental aspects</topic><topic>Genetic aspects</topic><topic>Identification and classification</topic><topic>Kinematics</topic><topic>Spermatozoa</topic><toplevel>online_resources</toplevel><creatorcontrib>Valverde, Anthony</creatorcontrib><creatorcontrib>Castro-Morales, Olivier</creatorcontrib><creatorcontrib>Madrigal-Valverde, Mónica</creatorcontrib><creatorcontrib>Camacho, Marlen</creatorcontrib><creatorcontrib>Barquero, Vinicio</creatorcontrib><creatorcontrib>Soler, Carles</creatorcontrib><creatorcontrib>Roldan, Eduardo R. S</creatorcontrib></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valverde, Anthony</au><au>Castro-Morales, Olivier</au><au>Madrigal-Valverde, Mónica</au><au>Camacho, Marlen</au><au>Barquero, Vinicio</au><au>Soler, Carles</au><au>Roldan, Eduardo R. S</au><format>book</format><genre>unknown</genre><ristype>RPRT</ristype><atitle>Sperm kinematic subpopulations of the American crocodile</atitle><jtitle>PLoS ONE</jtitle><date>2021-03-09</date><risdate>2021</risdate><volume>16</volume><issue>3</issue><spage>e0248270</spage><pages>e0248270-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>There has been very limited use of computer assisted semen analysis (CASA) to evaluate reptile sperm. The aim of this study was to examine sperm kinematic variables in American crocodile (Crocodylus acutus) semen samples and to assess whether sperm subpopulations could be characterized. Eight ejaculates (two ejaculates/male) from four sexually mature captive crocodiles were obtained. An ISAS®v1 CASA-Mot system, with an image acquisition rate of 50 Hz, and ISAS®D4C20 counting chambers were used for sperm analyses. The percentages of motile and progressively motile spermatozoa did not differ among animals (P > 0.05) but there was a significant animal effect with regards to kinematic variables (P < 0.05). Principal component (PC) analysis revealed that kinematic variables grouped into three components: PC1, related to velocity; PC2 to progressiveness and PC3 to oscillation. Subpopulation structure analysis identified four groups (P < 0.05), which represented, on average, 9.8%, 32.1%, 26.8%, and 31.3% of the total sperm population. Males differed in the proportion of sperm in each of the kinematic subpopulations. This new approach for the analysis of reptile sperm kinematic subpopulations, reflecting quantifiable parameters generated by CASA system technology, opens up possibilities for future assessments of crocodile sperm and will be useful in the future development of assisted reproduction for these species.</abstract><pub>Public Library of Science</pub><doi>10.1371/journal.pone.0248270</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PLoS ONE, 2021, Vol.16 (3), p.e0248270 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_gale_infotracacademiconefile_A654395294 |
| source | Public Library of Science (PLoS) Journals Open Access; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Distribution Environmental aspects Genetic aspects Identification and classification Kinematics Spermatozoa |
| title | Sperm kinematic subpopulations of the American crocodile |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T21%3A14%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=unknown&rft.atitle=Sperm%20kinematic%20subpopulations%20of%20the%20American%20crocodile&rft.jtitle=PLoS%20ONE&rft.au=Valverde,%20Anthony&rft.date=2021-03-09&rft.volume=16&rft.issue=3&rft.spage=e0248270&rft.pages=e0248270-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0248270&rft_dat=%3Cgale%3EA654395294%3C/gale%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_galeid=A654395294&rfr_iscdi=true |