One-shot analysis of translated mammalian lncRNAs with AHARIBO

A vast portion of the mammalian genome is transcribed as long non-coding RNAs (lncRNAs) acting in the cytoplasm with largely unknown functions. Surprisingly, lncRNAs have been shown to interact with ribosomes, encode peptides, or act as ribosome sponges. These functions still remain mostly undetecte...

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Veröffentlicht in:	eLife 2021-02, Vol.10
Hauptverfasser:	Minati, Luca, Firrito, Claudia, Del Piano, Alessia, Peretti, Alberto, Sidoli, Simone, Peroni, Daniele, Belli, Romina, Gandolfi, Francesco, Romanel, Alessandro, Bernabo, Paola, Zasso, Jacopo, Quattrone, Alessandro, Guella, Graziano, Lauria, Fabio, Viero, Gabriella, Clamer, Massimiliano
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal genetics Animals Cell Biology Cell differentiation Cytoplasm Efficiency Embryo cells Embryonic stem cells Gene expression Genomes Genomics Labeling LncRNA Mass spectrometry Mice Mouse Embryonic Stem Cells Non-coding RNA Peptides Peptides - metabolism Polypeptides Protein Biosynthesis Proteins proteogenomics Proteomics Protocol RIBO-seq ribosome Ribosomes Ribosomes - genetics Ribosomes - metabolism RNA RNA, Long Noncoding - genetics RNA, Long Noncoding - metabolism Scientific imaging Stem cell transplantation Stem cells Sucrose Tools and Resources translation
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