Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system
Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and E.sup.rns are major targets for eliciting antibodies against CSFV in infected animals. In this repo...
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| Veröffentlicht in: | Virology journal 2021-02, Vol.18 (1), p.1-44, Article 44 |
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| creator | Wei, Qiang Bai, Yilin Song, Yapeng Liu, Yunchao Yu, Wei Sun, Yaning Wang, Li Deng, Ruiguang Xing, Guangxu Zhang, Gaiping |
| description | Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and E.sup.rns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and E.sup.rns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E.sup.rns, or their combination (CSFV-E2 + E.sup.rns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID.sub.50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + E.sup.rns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the E.sup.rns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the E.sup.rns alone is not able to induce a protective immune response. Taken together, while the E.sup.rns could not confer protection against CSFV, E2 and E2 + E.sup.rns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits. |
| doi_str_mv | 10.1186/s12985-021-01507-1 |
| format | Article |
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The viral envelope glycoprotein E2 and E.sup.rns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and E.sup.rns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E.sup.rns, or their combination (CSFV-E2 + E.sup.rns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID.sub.50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + E.sup.rns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the E.sup.rns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the E.sup.rns alone is not able to induce a protective immune response. Taken together, while the E.sup.rns could not confer protection against CSFV, E2 and E2 + E.sup.rns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.</description><identifier>ISSN: 1743-422X</identifier><identifier>EISSN: 1743-422X</identifier><identifier>DOI: 10.1186/s12985-021-01507-1</identifier><identifier>PMID: 33627167</identifier><language>eng</language><publisher>London: BioMed Central Ltd</publisher><subject>Antibodies ; Antigens ; Care and treatment ; Chromatography ; Classical swine fever virus ; Cloning ; Development and progression ; Erns ; Fever ; Gene expression ; Genetic aspects ; Glycoprotein E2 ; Glycoproteins ; Health aspects ; Hog cholera ; Host-virus relationships ; Immune response ; Immune response (cell-mediated) ; Immune response (humoral) ; Immunization ; Immunogenicity ; Plasmids ; Proteins ; Rabbits ; Short Report ; Vaccines</subject><ispartof>Virology journal, 2021-02, Vol.18 (1), p.1-44, Article 44</ispartof><rights>COPYRIGHT 2021 BioMed Central Ltd.</rights><rights>2021. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-ab656dd6059d5fa2514934863385020baf6f9dbc081d005d1d7d208a5f0a88d23</citedby><cites>FETCH-LOGICAL-c504t-ab656dd6059d5fa2514934863385020baf6f9dbc081d005d1d7d208a5f0a88d23</cites><orcidid>0000-0002-1782-874X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903030/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7903030/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2100,27923,27924,53790,53792</link.rule.ids></links><search><creatorcontrib>Wei, Qiang</creatorcontrib><creatorcontrib>Bai, Yilin</creatorcontrib><creatorcontrib>Song, Yapeng</creatorcontrib><creatorcontrib>Liu, Yunchao</creatorcontrib><creatorcontrib>Yu, Wei</creatorcontrib><creatorcontrib>Sun, Yaning</creatorcontrib><creatorcontrib>Wang, Li</creatorcontrib><creatorcontrib>Deng, Ruiguang</creatorcontrib><creatorcontrib>Xing, Guangxu</creatorcontrib><creatorcontrib>Zhang, Gaiping</creatorcontrib><title>Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system</title><title>Virology journal</title><description>Classical swine fever (CSF) caused by the classical swine fever virus (CSFV) is a highly contagious swine disease resulting in large economical losses worldwide. The viral envelope glycoprotein E2 and E.sup.rns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and E.sup.rns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E.sup.rns, or their combination (CSFV-E2 + E.sup.rns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID.sub.50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + E.sup.rns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the E.sup.rns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the E.sup.rns alone is not able to induce a protective immune response. Taken together, while the E.sup.rns could not confer protection against CSFV, E2 and E2 + E.sup.rns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Care and treatment</subject><subject>Chromatography</subject><subject>Classical swine fever virus</subject><subject>Cloning</subject><subject>Development and progression</subject><subject>Erns</subject><subject>Fever</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Glycoprotein E2</subject><subject>Glycoproteins</subject><subject>Health aspects</subject><subject>Hog cholera</subject><subject>Host-virus relationships</subject><subject>Immune response</subject><subject>Immune response (cell-mediated)</subject><subject>Immune response (humoral)</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Rabbits</subject><subject>Short Report</subject><subject>Vaccines</subject><issn>1743-422X</issn><issn>1743-422X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptkk2P0zAQhiMEYpfCH-AUiQscsvgjdpwL0mpVlkorIfEhcbMce1xcJXaxk-72h_B_cdoKKEJzsDV-5rVn_BbFS4yuMBb8bcKkFaxCBFcIM9RU-FFxiZuaVjUh3x7_tb8onqW0QYgS3rRPiwtKOWkwby6Ln7fgIarRBV8qb0o3DJMPa_BOu3GfU6rfJ5fKYMsIOgyd88qPpe5VSk6rvkz3zkNpYQex3Lk4pXLd73XYxjCC8-WSHGSX0acSHrYRUoJ8iy87pac-HCtOB_Mb0j6NMDwvnljVJ3hxWhfF1_fLLzcfqruPt6ub67tKM1SPleo448ZwxFrDrCIM1y2tBadUMERQpyy3rek0EtggxAw2jSFIKGaREsIQuihWR10T1EZuoxtU3MugnDwkQlxLFUene5Aiq5FatMQIXnNDW7CtpcwCUNEJpLPWu6PWduoGMBr8GFV_Jnp-4t13uQ472bSIzrEoXp8EYvgxQRrl4JKGvlcewpQkmZtjtG1m9NU_6CZMMf9VpnLnbZP9Uf-h1io34LwN-V49i8przijPc2tYpq7-Q-UwMDgdPFiX82cFb84KMjPCw7hWU0py9fnTOUuOrI4hpQj29zwwkrOJ5dHEMptYHkwsMf0FDiblBQ</recordid><startdate>20210224</startdate><enddate>20210224</enddate><creator>Wei, Qiang</creator><creator>Bai, Yilin</creator><creator>Song, Yapeng</creator><creator>Liu, Yunchao</creator><creator>Yu, Wei</creator><creator>Sun, Yaning</creator><creator>Wang, Li</creator><creator>Deng, Ruiguang</creator><creator>Xing, Guangxu</creator><creator>Zhang, Gaiping</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-1782-874X</orcidid></search><sort><creationdate>20210224</creationdate><title>Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system</title><author>Wei, Qiang ; 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The viral envelope glycoprotein E2 and E.sup.rns are major targets for eliciting antibodies against CSFV in infected animals. In this report, the glycoprotein E2 and E.sup.rns were expressed using the baculovirus system and their protective immunity in rabbits were tested. Twenty CSFV seronegative rabbits were randomly divided into five groups. Each rabbit was intramuscularly immunized with CSFV-E2, CSFV-E.sup.rns, or their combination (CSFV-E2 + E.sup.rns). Besides, a commercial CSFV vaccine (C-strain) and PBS were used as positive or negative controls, respectively. Four weeks after the second immunization, all the rabbits were challenged with 100 RID.sub.50 of CSFV C-strain. High levels of CSFV E2-specific antibody, neutralizing antibody and cellular immune responses to CSFV were elicited in the rabbits inoculated with C-strain, CSFV-E2, and CSFV-E2 + E.sup.rns. And the rabbits inoculated with the three vaccines received complete protection against CSFV C-strain. However, no neutralizing antibody was detected in the E.sup.rns vaccinated rabbits and the rabbits exhibited fever typical of CSFV, suggesting the E.sup.rns alone is not able to induce a protective immune response. Taken together, while the E.sup.rns could not confer protection against CSFV, E2 and E2 + E.sup.rns could not only elicit humoral and cell-mediated immune responses but also confer complete protection against CSFV C-strain in rabbits.</abstract><cop>London</cop><pub>BioMed Central Ltd</pub><pmid>33627167</pmid><doi>10.1186/s12985-021-01507-1</doi><orcidid>https://orcid.org/0000-0002-1782-874X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Antibodies Antigens Care and treatment Chromatography Classical swine fever virus Cloning Development and progression Erns Fever Gene expression Genetic aspects Glycoprotein E2 Glycoproteins Health aspects Hog cholera Host-virus relationships Immune response Immune response (cell-mediated) Immune response (humoral) Immunization Immunogenicity Plasmids Proteins Rabbits Short Report Vaccines |
| title | Generation and immunogenicity analysis of recombinant classical swine fever virus glycoprotein E2 and Erns expressed in baculovirus expression system |
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