Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells
The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the prese...
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| creator | Suwabe, Yoko Nakano, Rei Namba, Shinichi Yachiku, Naoya Kuji, Manami Sugimura, Mana Kitanaka, Nanako Kitanaka, Taku Konno, Tadayoshi Sugiya, Hiroshi Nakayama, Tomohiro |
| description | The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. These findings suggest that glucose uptake via GLUT1 and GLUT3 plays a crucial role for the growth of canine melanoma cells. |
| doi_str_mv | 10.1371/journal.pone.0243859 |
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Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. These findings suggest that glucose uptake via GLUT1 and GLUT3 plays a crucial role for the growth of canine melanoma cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0243859</identifier><identifier>PMID: 33539362</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Biochemistry ; Biology and life sciences ; Biomedical materials ; Blood vessels ; Carbon dioxide ; Cell suspensions ; Chromosome number ; Chromosomes ; Cryopreservation ; Dehydrogenases ; Diseases ; Dogs ; Drug therapy ; Editing ; Fluorides ; Freezing ; Funding ; Genetic aspects ; Glucose ; Glucose metabolism ; Health aspects ; Incubation ; Laboratories ; Mammalian cells ; Medicine and Health Sciences ; Melanoma ; Metabolism ; Pharmaceuticals ; Physical Sciences ; Radiation therapy ; Reagents ; Research and Analysis Methods ; Reviews ; Skin cancer ; Tubes ; Water baths</subject><ispartof>PloS one, 2021-02, Vol.16 (2), p.e0243859-e0243859</ispartof><rights>COPYRIGHT 2021 Public Library of Science</rights><rights>2021 Suwabe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. 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Manami</au><au>Sugimura, Mana</au><au>Kitanaka, Nanako</au><au>Kitanaka, Taku</au><au>Konno, Tadayoshi</au><au>Sugiya, Hiroshi</au><au>Nakayama, Tomohiro</au><au>Pizzo, Salvatore V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2021-02-04</date><risdate>2021</risdate><volume>16</volume><issue>2</issue><spage>e0243859</spage><epage>e0243859</epage><pages>e0243859-e0243859</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The rate of glucose uptake dramatically increases in cancer cells even in the presence of oxygen and fully functioning mitochondria. Cancer cells produce ATP by glycolysis rather than oxidative phosphorylation under aerobic conditions, a process termed as the "Warburg effect." In the present study, we treated canine melanoma cells with the glucose analog 2-deoxy-D-glucose (2-DG) and investigated its effect on cell growth. 2-DG attenuated cell growth in a time- and dose-dependent manner. Cell growth was also inhibited following treatment with the glucose transporter (GLUT) inhibitor WZB-117. The treatment of 2-DG and WZB-117 attenuated the glucose consumption, lactate secretion and glucose uptake of the cells. The mRNA expression of the subtypes of GLUT was examined and GLUT1 and GLUT3 were found to be expressed in melanoma cells. The growth, glucose consumption and lactate secretion of melanoma cells transfected with siRNAs of specific for GLUT1 and GLUT3 was suppressed. These findings suggest that glucose uptake via GLUT1 and GLUT3 plays a crucial role for the growth of canine melanoma cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>33539362</pmid><doi>10.1371/journal.pone.0243859</doi><tpages>e0243859</tpages><orcidid>https://orcid.org/0000-0002-0435-5314</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biology and life sciences Biomedical materials Blood vessels Carbon dioxide Cell suspensions Chromosome number Chromosomes Cryopreservation Dehydrogenases Diseases Dogs Drug therapy Editing Fluorides Freezing Funding Genetic aspects Glucose Glucose metabolism Health aspects Incubation Laboratories Mammalian cells Medicine and Health Sciences Melanoma Metabolism Pharmaceuticals Physical Sciences Radiation therapy Reagents Research and Analysis Methods Reviews Skin cancer Tubes Water baths |
| title | Involvement of GLUT1 and GLUT3 in the growth of canine melanoma cells |
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