Detection and Control of Biofilm Formation by Staphylococcus aureus from Febrile Neutropenic Patient
Febrile neutropenia (FN) is the evolution of fever in a patient with neutropenia over 38.0°C. Neutropenia is diagnosed when absolute neutrophil count (ANC)
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| creator | El-Nagdy, Ahmed Hazem Abdel-Fattah, Gamal M Emarah, Ziad |
| description | Febrile neutropenia (FN) is the evolution of fever in a patient with neutropenia over 38.0°C. Neutropenia is diagnosed when absolute neutrophil count (ANC) |
| doi_str_mv | 10.2147/IDR.S259914 |
| format | Article |
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is one of the most important microorganisms isolated from the blood of febrile neutropenic patients. Infections caused by
range from mild to life-threatening diseases. Biofilm production by
is one of the most significant virulence factors of the bacterium as it prevents the penetration of antibiotics. Recently, it has been shown that
carries the
operon responsible for biofilm production. The aim of the work is to determine a genotypic characterization that includes not only the detection of
and
genes in
but also the determination of their relation to clinical and microbiological features. Empiric antibacterial treatment was recommended for cancer patients receiving chemotherapy.
The relation between the presence of
and
and biofilm production was determined in a collection of 66
samples from febrile neutropenic patients. Biofilm-forming ability was tested on Congo Red agar plates. Also, the effect of the most sensitive antibiotics on the bacterial cells was determined by an electron microscope.
Of the bacterial samples, 48 were biofilm-productive and 18 were non-biofilm productive. For the biofilm productive bacteria, 37.5% were positive for
, 22.9% were positive for
and 10.4% were positive for both. Linezolid was the most effective antibiotic and it is highly recommended for the treatment of febrile neutropenia caused by biofilm-productive
. Severe changes were found on the bacterial cell after being treated with Linezolid. The
and
genes were present in only 50% of biofilm-productive bacteria.
The
operon is present in only 50% of biofilm-productive
and Linezolid is the best antibiotic against these bacteria.</description><identifier>ISSN: 1178-6973</identifier><identifier>EISSN: 1178-6973</identifier><identifier>DOI: 10.2147/IDR.S259914</identifier><identifier>PMID: 32982324</identifier><language>eng</language><publisher>New Zealand: Dove Medical Press Limited</publisher><subject>Antibacterial agents ; Antibiotics ; Bacteria ; biofilm production ; Biofilms ; Body temperature ; Cancer ; Cancer patients ; Cancer treatment ; Chemotherapy ; Chromosomes ; Congo (Kinshasa) ; Egypt ; Enzymes ; Ethanol ; febrile neutropenia ; Fever ; Gene expression ; Health aspects ; icaa ; icad ; Infection ; Laboratories ; Linezolid ; Morbidity ; Mortality ; Neutropenia ; Neutrophils ; Original Research ; Patients ; Staphylococcus aureus ; Staphylococcus aureus infections ; Staphylococcus infections ; Virulence (Microbiology) ; Virulence factors</subject><ispartof>Infection and drug resistance, 2020-01, Vol.13, p.3091-3101</ispartof><rights>2020 El-Nagdy et al.</rights><rights>COPYRIGHT 2020 Dove Medical Press Limited</rights><rights>2020. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2020 El-Nagdy et al. 2020 El-Nagdy et al.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c573t-73487e8906ddbdd5402100646134da0b1ef78c42a69fad7f4a4ddaeacc6925153</citedby><cites>FETCH-LOGICAL-c573t-73487e8906ddbdd5402100646134da0b1ef78c42a69fad7f4a4ddaeacc6925153</cites><orcidid>0000-0002-6669-2180 ; 0000-0003-0622-2598</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495500/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7495500/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,3849,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32982324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Nagdy, Ahmed Hazem</creatorcontrib><creatorcontrib>Abdel-Fattah, Gamal M</creatorcontrib><creatorcontrib>Emarah, Ziad</creatorcontrib><title>Detection and Control of Biofilm Formation by Staphylococcus aureus from Febrile Neutropenic Patient</title><title>Infection and drug resistance</title><addtitle>Infect Drug Resist</addtitle><description>Febrile neutropenia (FN) is the evolution of fever in a patient with neutropenia over 38.0°C. Neutropenia is diagnosed when absolute neutrophil count (ANC) <1500 cells/µL. FN represents a common complication of cancer treatment. Hence, it is featured to be a major cause of morbidity and mortality in cancer patients.
is one of the most important microorganisms isolated from the blood of febrile neutropenic patients. Infections caused by
range from mild to life-threatening diseases. Biofilm production by
is one of the most significant virulence factors of the bacterium as it prevents the penetration of antibiotics. Recently, it has been shown that
carries the
operon responsible for biofilm production. The aim of the work is to determine a genotypic characterization that includes not only the detection of
and
genes in
but also the determination of their relation to clinical and microbiological features. Empiric antibacterial treatment was recommended for cancer patients receiving chemotherapy.
The relation between the presence of
and
and biofilm production was determined in a collection of 66
samples from febrile neutropenic patients. Biofilm-forming ability was tested on Congo Red agar plates. Also, the effect of the most sensitive antibiotics on the bacterial cells was determined by an electron microscope.
Of the bacterial samples, 48 were biofilm-productive and 18 were non-biofilm productive. For the biofilm productive bacteria, 37.5% were positive for
, 22.9% were positive for
and 10.4% were positive for both. Linezolid was the most effective antibiotic and it is highly recommended for the treatment of febrile neutropenia caused by biofilm-productive
. Severe changes were found on the bacterial cell after being treated with Linezolid. The
and
genes were present in only 50% of biofilm-productive bacteria.
The
operon is present in only 50% of biofilm-productive
and Linezolid is the best antibiotic against these bacteria.</description><subject>Antibacterial agents</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>biofilm production</subject><subject>Biofilms</subject><subject>Body temperature</subject><subject>Cancer</subject><subject>Cancer patients</subject><subject>Cancer treatment</subject><subject>Chemotherapy</subject><subject>Chromosomes</subject><subject>Congo (Kinshasa)</subject><subject>Egypt</subject><subject>Enzymes</subject><subject>Ethanol</subject><subject>febrile neutropenia</subject><subject>Fever</subject><subject>Gene expression</subject><subject>Health aspects</subject><subject>icaa</subject><subject>icad</subject><subject>Infection</subject><subject>Laboratories</subject><subject>Linezolid</subject><subject>Morbidity</subject><subject>Mortality</subject><subject>Neutropenia</subject><subject>Neutrophils</subject><subject>Original Research</subject><subject>Patients</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus infections</subject><subject>Staphylococcus infections</subject><subject>Virulence (Microbiology)</subject><subject>Virulence factors</subject><issn>1178-6973</issn><issn>1178-6973</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><sourceid>DOA</sourceid><recordid>eNptkt-LEzEQxxdRvOO8J99lQRBBWvNrk82LcPY8LRwqnj6HbDJpU7Kbmt0V-t-bvdazFZOHCclnvmFmvkXxHKM5wUy8XV5_m9-RSkrMHhXnGIt6xqWgj4_OZ8Vl329QXlRyJsjT4owSWRNK2Hlhr2EAM_jYlbqz5SJ2Q4qhjK5876PzoS1vYmr1PdDsyrtBb9e7EE00ZuxLPSbIwaWYOWiSD1B-hjFLbKHzpvyaE6EbnhVPnA49XB7iRfHj5sP3xafZ7ZePy8XV7cxUgg4zQVktoJaIW9tYWzFEMEKccUyZ1ajB4ERtGNFcOm2FY5pZq0EbwyWpcEUviuVe10a9UdvkW512Kmqv7i9iWimdBm8CKIEcw41snICG0QrVwLFmFQMBXDiOsta7vdZ2bFqwJpeRdDgRPX3p_Fqt4i8lmKwqNAm8Pgik-HOEflCt7w2EoDuIY68IY1zWeT48oy__QTdxTF1u1UQJTgiR9C-10rkA37mY_zWTqLritK4ZrxjL1Pw_VN4WWm9iB3mocJrw6ihhDToM6z6GcRp5fwq-2YMmxb5P4B6agZGazKiyGdXBjJl-cdy_B_aP9ehvHIjYKg</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>El-Nagdy, Ahmed Hazem</creator><creator>Abdel-Fattah, Gamal M</creator><creator>Emarah, Ziad</creator><general>Dove Medical Press Limited</general><general>Taylor & Francis Ltd</general><general>Dove</general><general>Dove Medical Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-6669-2180</orcidid><orcidid>https://orcid.org/0000-0003-0622-2598</orcidid></search><sort><creationdate>20200101</creationdate><title>Detection and Control of Biofilm Formation by Staphylococcus aureus from Febrile Neutropenic Patient</title><author>El-Nagdy, Ahmed Hazem ; 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Neutropenia is diagnosed when absolute neutrophil count (ANC) <1500 cells/µL. FN represents a common complication of cancer treatment. Hence, it is featured to be a major cause of morbidity and mortality in cancer patients.
is one of the most important microorganisms isolated from the blood of febrile neutropenic patients. Infections caused by
range from mild to life-threatening diseases. Biofilm production by
is one of the most significant virulence factors of the bacterium as it prevents the penetration of antibiotics. Recently, it has been shown that
carries the
operon responsible for biofilm production. The aim of the work is to determine a genotypic characterization that includes not only the detection of
and
genes in
but also the determination of their relation to clinical and microbiological features. Empiric antibacterial treatment was recommended for cancer patients receiving chemotherapy.
The relation between the presence of
and
and biofilm production was determined in a collection of 66
samples from febrile neutropenic patients. Biofilm-forming ability was tested on Congo Red agar plates. Also, the effect of the most sensitive antibiotics on the bacterial cells was determined by an electron microscope.
Of the bacterial samples, 48 were biofilm-productive and 18 were non-biofilm productive. For the biofilm productive bacteria, 37.5% were positive for
, 22.9% were positive for
and 10.4% were positive for both. Linezolid was the most effective antibiotic and it is highly recommended for the treatment of febrile neutropenia caused by biofilm-productive
. Severe changes were found on the bacterial cell after being treated with Linezolid. The
and
genes were present in only 50% of biofilm-productive bacteria.
The
operon is present in only 50% of biofilm-productive
and Linezolid is the best antibiotic against these bacteria.</abstract><cop>New Zealand</cop><pub>Dove Medical Press Limited</pub><pmid>32982324</pmid><doi>10.2147/IDR.S259914</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6669-2180</orcidid><orcidid>https://orcid.org/0000-0003-0622-2598</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Taylor & Francis Open Access; DOVE Medical Press Journals; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access |
| subjects | Antibacterial agents Antibiotics Bacteria biofilm production Biofilms Body temperature Cancer Cancer patients Cancer treatment Chemotherapy Chromosomes Congo (Kinshasa) Egypt Enzymes Ethanol febrile neutropenia Fever Gene expression Health aspects icaa icad Infection Laboratories Linezolid Morbidity Mortality Neutropenia Neutrophils Original Research Patients Staphylococcus aureus Staphylococcus aureus infections Staphylococcus infections Virulence (Microbiology) Virulence factors |
| title | Detection and Control of Biofilm Formation by Staphylococcus aureus from Febrile Neutropenic Patient |
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