Acetosyringone's Effect on Agrobacterium-mediated Transformation of Eustoma grandiflorum Leaf Disks

Eustoma is the most popular ornamental flower in the world. It is expected that the production of transgenic Eustoma will contribute to the study and modification of its characteristics, such as growth, flowering time, flower color and flower shape. Agrobacterium-mediated leaf disc transformation is a commol/Lon method of plant transformation. In order to improve the transformation efficiency of Eustoma, I concentrated on two steps: inoculation of Agrobacterium to the leaf disc and then co-cultivation. The effect of the detergent in the inoculation buffer was tested. Tween 20 was identified as a better candidate for use in the buffer, given its marginal impact on disk survivability. Acetyl syringone is known to increase conversion efficiency by upregulating the virulence ofAgrobacterium. In previous studies using Eustoma, the effect of this compound was tested only during the inoculation process. However, I studied the effect of acetosyringone by adding it to the inoculum and co-cultivation medium. When added to the co-cultivation medium, acetosyringone significantly increased the number of transformed callus bearing the labeled transgene. By adding acetosyringone, the callus of each leaf disc was greater than 0.7, and in the absence of each, the callus of each disc was less than 0.03. Thus, transgene-mediated incorporation into the Eustoma genome can be enhanced by the addition of acetosyringone to the co-cultivation medium. Keywords Agrobacterium tumefaciens.