High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy
Neurotropic viral transsynaptic tracing is an increasingly powerful technique for dissecting the structure and function of neural circuits. Herpes simplex virus type 1 strain H129 has been widely used as an anterograde tracer. However, HSV tracers still have several shortcomings, including high toxi...
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| Veröffentlicht in: | Molecular brain 2020-01, Vol.13 (1), p.5-5, Article 5 |
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| creator | Su, Peng Ying, Min Han, Zengpeng Xia, Jinjin Jin, Sen Li, Yingli Wang, Huadong Xu, Fuqiang |
| description | Neurotropic viral transsynaptic tracing is an increasingly powerful technique for dissecting the structure and function of neural circuits. Herpes simplex virus type 1 strain H129 has been widely used as an anterograde tracer. However, HSV tracers still have several shortcomings, including high toxicity, low sensitivity and non-specific retrograde labeling. Here, we aimed to construct high-brightness HSV anterograde tracers by increasing the expression of exogenous genes carried by H129 viruses. Using a Trojan horse-like strategy, a HSV/AAV (adeno-associated virus) chimaera termed H8 was generated to enhance the expression of a fluorescent marker. In vitro and in vivo assays showed that the exogenous gene was efficiently replicated and amplified by the synergism of the HSV vector and introduced AAV replication system. H8 reporting fluorescence was brighter than that of currently available H129 tracers, and H8 could be used for fast and effective anterograde tracing without additional immunostaining. These results indicated that foreign gene expression in HSV tracers could be enhanced by integrating HSV with AAV replication system. This approach may be useful as a general enhanced expression strategy for HSV-based tracing tools or gene delivery vectors. |
| doi_str_mv | 10.1186/s13041-020-0544-2 |
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Herpes simplex virus type 1 strain H129 has been widely used as an anterograde tracer. However, HSV tracers still have several shortcomings, including high toxicity, low sensitivity and non-specific retrograde labeling. Here, we aimed to construct high-brightness HSV anterograde tracers by increasing the expression of exogenous genes carried by H129 viruses. Using a Trojan horse-like strategy, a HSV/AAV (adeno-associated virus) chimaera termed H8 was generated to enhance the expression of a fluorescent marker. In vitro and in vivo assays showed that the exogenous gene was efficiently replicated and amplified by the synergism of the HSV vector and introduced AAV replication system. H8 reporting fluorescence was brighter than that of currently available H129 tracers, and H8 could be used for fast and effective anterograde tracing without additional immunostaining. These results indicated that foreign gene expression in HSV tracers could be enhanced by integrating HSV with AAV replication system. This approach may be useful as a general enhanced expression strategy for HSV-based tracing tools or gene delivery vectors.</description><identifier>ISSN: 1756-6606</identifier><identifier>EISSN: 1756-6606</identifier><identifier>DOI: 10.1186/s13041-020-0544-2</identifier><identifier>PMID: 31931837</identifier><language>eng</language><publisher>LONDON: Springer Nature</publisher><subject>Adeno-associated virus ; Animals ; Anterograde tracer ; Antibiotics ; Axonal Transport - physiology ; Brain - cytology ; Brightness ; Cell Line ; Cell Nucleus - virology ; Defective Viruses - genetics ; Defective Viruses - physiology ; Deoxyribonucleic acid ; Dependovirus - genetics ; Dependovirus - physiology ; DNA ; Expression vectors ; Fluorescence ; Fluorescent indicators ; Gene expression ; Gene therapy ; Gene transfer ; Genes ; Genes, Reporter ; Genes, Synthetic ; Genetic research ; Genetic vectors ; Genomes ; Green Fluorescent Proteins - analysis ; Green Fluorescent Proteins - biosynthesis ; Green Fluorescent Proteins - genetics ; H129 ; Helper Viruses - genetics ; Helper Viruses - physiology ; Herpes simplex ; Herpes simplex virus ; Herpes viruses ; Herpesvirus 1, Human - genetics ; Herpesvirus 1, Human - physiology ; Herpesvirus infections ; Infections ; Laboratory animals ; Life Sciences & Biomedicine ; Male ; Malware ; Methodology ; Mice ; Mice, Inbred C57BL ; Neural circuit tracing ; Neural circuitry ; Neural networks ; Neural Pathways - ultrastructure ; Neuroanatomical Tract-Tracing Techniques - methods ; Neuronal Tract-Tracers - analysis ; Neurons - ultrastructure ; Neurons - virology ; Neurosciences ; Neurosciences & Neurology ; Plasmids ; Protein expression ; Proteins ; Reassortant Viruses - genetics ; Reassortant Viruses - physiology ; Replication ; Science & Technology ; Structure-function relationships ; Toxicity ; Tracers ; Tracers (Biology) ; Trojan horse-like strategy ; Viral Replicase Complex Proteins - genetics ; Virus Replication</subject><ispartof>Molecular brain, 2020-01, Vol.13 (1), p.5-5, Article 5</ispartof><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s). 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>15</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000513655100002</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c560t-a4c7bd359b13a81d9605ef1db243d85dc76b68bac92b5eb32803582767fee9153</citedby><cites>FETCH-LOGICAL-c560t-a4c7bd359b13a81d9605ef1db243d85dc76b68bac92b5eb32803582767fee9153</cites><orcidid>0000-0001-8076-3152 ; 0000-0003-2388-6062</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958791/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6958791/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2103,2115,27928,27929,53795,53797</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31931837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Peng</creatorcontrib><creatorcontrib>Ying, Min</creatorcontrib><creatorcontrib>Han, Zengpeng</creatorcontrib><creatorcontrib>Xia, Jinjin</creatorcontrib><creatorcontrib>Jin, Sen</creatorcontrib><creatorcontrib>Li, Yingli</creatorcontrib><creatorcontrib>Wang, Huadong</creatorcontrib><creatorcontrib>Xu, Fuqiang</creatorcontrib><title>High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy</title><title>Molecular brain</title><addtitle>MOL BRAIN</addtitle><addtitle>Mol Brain</addtitle><description>Neurotropic viral transsynaptic tracing is an increasingly powerful technique for dissecting the structure and function of neural circuits. Herpes simplex virus type 1 strain H129 has been widely used as an anterograde tracer. However, HSV tracers still have several shortcomings, including high toxicity, low sensitivity and non-specific retrograde labeling. Here, we aimed to construct high-brightness HSV anterograde tracers by increasing the expression of exogenous genes carried by H129 viruses. Using a Trojan horse-like strategy, a HSV/AAV (adeno-associated virus) chimaera termed H8 was generated to enhance the expression of a fluorescent marker. In vitro and in vivo assays showed that the exogenous gene was efficiently replicated and amplified by the synergism of the HSV vector and introduced AAV replication system. H8 reporting fluorescence was brighter than that of currently available H129 tracers, and H8 could be used for fast and effective anterograde tracing without additional immunostaining. These results indicated that foreign gene expression in HSV tracers could be enhanced by integrating HSV with AAV replication system. This approach may be useful as a general enhanced expression strategy for HSV-based tracing tools or gene delivery vectors.</description><subject>Adeno-associated virus</subject><subject>Animals</subject><subject>Anterograde tracer</subject><subject>Antibiotics</subject><subject>Axonal Transport - physiology</subject><subject>Brain - cytology</subject><subject>Brightness</subject><subject>Cell Line</subject><subject>Cell Nucleus - virology</subject><subject>Defective Viruses - genetics</subject><subject>Defective Viruses - physiology</subject><subject>Deoxyribonucleic acid</subject><subject>Dependovirus - genetics</subject><subject>Dependovirus - physiology</subject><subject>DNA</subject><subject>Expression vectors</subject><subject>Fluorescence</subject><subject>Fluorescent indicators</subject><subject>Gene expression</subject><subject>Gene therapy</subject><subject>Gene transfer</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Genes, Synthetic</subject><subject>Genetic research</subject><subject>Genetic vectors</subject><subject>Genomes</subject><subject>Green Fluorescent Proteins - analysis</subject><subject>Green Fluorescent Proteins - biosynthesis</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>H129</subject><subject>Helper Viruses - genetics</subject><subject>Helper Viruses - physiology</subject><subject>Herpes simplex</subject><subject>Herpes simplex virus</subject><subject>Herpes viruses</subject><subject>Herpesvirus 1, Human - genetics</subject><subject>Herpesvirus 1, Human - physiology</subject><subject>Herpesvirus infections</subject><subject>Infections</subject><subject>Laboratory animals</subject><subject>Life Sciences & Biomedicine</subject><subject>Male</subject><subject>Malware</subject><subject>Methodology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Neural circuit tracing</subject><subject>Neural circuitry</subject><subject>Neural networks</subject><subject>Neural Pathways - ultrastructure</subject><subject>Neuroanatomical Tract-Tracing Techniques - methods</subject><subject>Neuronal Tract-Tracers - analysis</subject><subject>Neurons - ultrastructure</subject><subject>Neurons - virology</subject><subject>Neurosciences</subject><subject>Neurosciences & Neurology</subject><subject>Plasmids</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Reassortant Viruses - genetics</subject><subject>Reassortant Viruses - physiology</subject><subject>Replication</subject><subject>Science & Technology</subject><subject>Structure-function relationships</subject><subject>Toxicity</subject><subject>Tracers</subject><subject>Tracers (Biology)</subject><subject>Trojan horse-like strategy</subject><subject>Viral Replicase Complex Proteins - genetics</subject><subject>Virus Replication</subject><issn>1756-6606</issn><issn>1756-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl1rFDEUhgdRbK3-AG9kwBtBpiaTydeNUBZ1CwUvrN6GfJyZzTqb1GRG6b8349a1K15IIAknz_uSk7xV9Ryjc4wFe5MxQR1uUIsaRLuuaR9Up5hT1jCG2MN7-5PqSc5bhFjLMH1cnRAsCRaEn1b92g-bxqQyTwFyrnWYIMUhaQf1lHTIAeYUgx7r9acvuF7jVi51C6neRed7D66esw9DrevrFLc61JuYMjSj_wp1LugEw-3T6lGvxwzP7taz6vP7d9erdXP18cPl6uKqsZShqdGd5cYRKg0mWmAnGaLQY2fajjhBneXMMGG0la2hYEgrEKGi5Yz3ABJTclZd7n1d1Ft1k_xOp1sVtVe_CjENSqfJ2xEUFRZ6ZAhxhnZGS6kJtw4RAx0H3Nni9XbvdTObHTgLoTQzHpkenwS_UUP8rpikgktcDF7dGaT4bYY8qZ3PFsZRB4hzVi0hAkks-XLvl3-h2zin8uoL1eGOSyLaP9SgSwM-9HH5icVUXTAsOBaU8UKd_4Mqw8HO2xig96V-JMB7gU0x5wT9oUeM1BI0tQ-aKkFTS9DUcpUX9x_noPidrAKIPfADTOyz9RAsHDCEEMWEUYrLDrUrP-nJx7CKc5iK9PX_S8lPHwXuBA</recordid><startdate>20200113</startdate><enddate>20200113</enddate><creator>Su, Peng</creator><creator>Ying, Min</creator><creator>Han, Zengpeng</creator><creator>Xia, Jinjin</creator><creator>Jin, Sen</creator><creator>Li, Yingli</creator><creator>Wang, Huadong</creator><creator>Xu, Fuqiang</creator><general>Springer Nature</general><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-8076-3152</orcidid><orcidid>https://orcid.org/0000-0003-2388-6062</orcidid></search><sort><creationdate>20200113</creationdate><title>High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy</title><author>Su, Peng ; Ying, Min ; Han, Zengpeng ; Xia, Jinjin ; Jin, Sen ; Li, Yingli ; Wang, Huadong ; Xu, Fuqiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c560t-a4c7bd359b13a81d9605ef1db243d85dc76b68bac92b5eb32803582767fee9153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adeno-associated virus</topic><topic>Animals</topic><topic>Anterograde tracer</topic><topic>Antibiotics</topic><topic>Axonal Transport - physiology</topic><topic>Brain - cytology</topic><topic>Brightness</topic><topic>Cell Line</topic><topic>Cell Nucleus - virology</topic><topic>Defective Viruses - genetics</topic><topic>Defective Viruses - physiology</topic><topic>Deoxyribonucleic acid</topic><topic>Dependovirus - genetics</topic><topic>Dependovirus - physiology</topic><topic>DNA</topic><topic>Expression vectors</topic><topic>Fluorescence</topic><topic>Fluorescent indicators</topic><topic>Gene expression</topic><topic>Gene therapy</topic><topic>Gene transfer</topic><topic>Genes</topic><topic>Genes, Reporter</topic><topic>Genes, Synthetic</topic><topic>Genetic research</topic><topic>Genetic vectors</topic><topic>Genomes</topic><topic>Green Fluorescent Proteins - analysis</topic><topic>Green Fluorescent Proteins - biosynthesis</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>H129</topic><topic>Helper Viruses - genetics</topic><topic>Helper Viruses - physiology</topic><topic>Herpes simplex</topic><topic>Herpes simplex virus</topic><topic>Herpes viruses</topic><topic>Herpesvirus 1, Human - genetics</topic><topic>Herpesvirus 1, Human - physiology</topic><topic>Herpesvirus infections</topic><topic>Infections</topic><topic>Laboratory animals</topic><topic>Life Sciences & Biomedicine</topic><topic>Male</topic><topic>Malware</topic><topic>Methodology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Neural circuit tracing</topic><topic>Neural circuitry</topic><topic>Neural networks</topic><topic>Neural Pathways - ultrastructure</topic><topic>Neuroanatomical Tract-Tracing Techniques - methods</topic><topic>Neuronal Tract-Tracers - analysis</topic><topic>Neurons - ultrastructure</topic><topic>Neurons - virology</topic><topic>Neurosciences</topic><topic>Neurosciences & Neurology</topic><topic>Plasmids</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Reassortant Viruses - genetics</topic><topic>Reassortant Viruses - physiology</topic><topic>Replication</topic><topic>Science & Technology</topic><topic>Structure-function relationships</topic><topic>Toxicity</topic><topic>Tracers</topic><topic>Tracers (Biology)</topic><topic>Trojan horse-like strategy</topic><topic>Viral Replicase Complex Proteins - genetics</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Su, Peng</creatorcontrib><creatorcontrib>Ying, Min</creatorcontrib><creatorcontrib>Han, Zengpeng</creatorcontrib><creatorcontrib>Xia, Jinjin</creatorcontrib><creatorcontrib>Jin, Sen</creatorcontrib><creatorcontrib>Li, Yingli</creatorcontrib><creatorcontrib>Wang, Huadong</creatorcontrib><creatorcontrib>Xu, Fuqiang</creatorcontrib><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>Proquest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Molecular brain</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Su, Peng</au><au>Ying, Min</au><au>Han, Zengpeng</au><au>Xia, Jinjin</au><au>Jin, Sen</au><au>Li, Yingli</au><au>Wang, Huadong</au><au>Xu, Fuqiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy</atitle><jtitle>Molecular brain</jtitle><stitle>MOL BRAIN</stitle><addtitle>Mol Brain</addtitle><date>2020-01-13</date><risdate>2020</risdate><volume>13</volume><issue>1</issue><spage>5</spage><epage>5</epage><pages>5-5</pages><artnum>5</artnum><issn>1756-6606</issn><eissn>1756-6606</eissn><abstract>Neurotropic viral transsynaptic tracing is an increasingly powerful technique for dissecting the structure and function of neural circuits. Herpes simplex virus type 1 strain H129 has been widely used as an anterograde tracer. However, HSV tracers still have several shortcomings, including high toxicity, low sensitivity and non-specific retrograde labeling. Here, we aimed to construct high-brightness HSV anterograde tracers by increasing the expression of exogenous genes carried by H129 viruses. Using a Trojan horse-like strategy, a HSV/AAV (adeno-associated virus) chimaera termed H8 was generated to enhance the expression of a fluorescent marker. In vitro and in vivo assays showed that the exogenous gene was efficiently replicated and amplified by the synergism of the HSV vector and introduced AAV replication system. H8 reporting fluorescence was brighter than that of currently available H129 tracers, and H8 could be used for fast and effective anterograde tracing without additional immunostaining. These results indicated that foreign gene expression in HSV tracers could be enhanced by integrating HSV with AAV replication system. This approach may be useful as a general enhanced expression strategy for HSV-based tracing tools or gene delivery vectors.</abstract><cop>LONDON</cop><pub>Springer Nature</pub><pmid>31931837</pmid><doi>10.1186/s13041-020-0544-2</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-8076-3152</orcidid><orcidid>https://orcid.org/0000-0003-2388-6062</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adeno-associated virus Animals Anterograde tracer Antibiotics Axonal Transport - physiology Brain - cytology Brightness Cell Line Cell Nucleus - virology Defective Viruses - genetics Defective Viruses - physiology Deoxyribonucleic acid Dependovirus - genetics Dependovirus - physiology DNA Expression vectors Fluorescence Fluorescent indicators Gene expression Gene therapy Gene transfer Genes Genes, Reporter Genes, Synthetic Genetic research Genetic vectors Genomes Green Fluorescent Proteins - analysis Green Fluorescent Proteins - biosynthesis Green Fluorescent Proteins - genetics H129 Helper Viruses - genetics Helper Viruses - physiology Herpes simplex Herpes simplex virus Herpes viruses Herpesvirus 1, Human - genetics Herpesvirus 1, Human - physiology Herpesvirus infections Infections Laboratory animals Life Sciences & Biomedicine Male Malware Methodology Mice Mice, Inbred C57BL Neural circuit tracing Neural circuitry Neural networks Neural Pathways - ultrastructure Neuroanatomical Tract-Tracing Techniques - methods Neuronal Tract-Tracers - analysis Neurons - ultrastructure Neurons - virology Neurosciences Neurosciences & Neurology Plasmids Protein expression Proteins Reassortant Viruses - genetics Reassortant Viruses - physiology Replication Science & Technology Structure-function relationships Toxicity Tracers Tracers (Biology) Trojan horse-like strategy Viral Replicase Complex Proteins - genetics Virus Replication |
| title | High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-17T04%3A20%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High-brightness%20anterograde%20transneuronal%20HSV1%20H129%20tracer%20modified%20using%20a%20Trojan%20horse-like%20strategy&rft.jtitle=Molecular%20brain&rft.au=Su,%20Peng&rft.date=2020-01-13&rft.volume=13&rft.issue=1&rft.spage=5&rft.epage=5&rft.pages=5-5&rft.artnum=5&rft.issn=1756-6606&rft.eissn=1756-6606&rft_id=info:doi/10.1186/s13041-020-0544-2&rft_dat=%3Cgale_doaj_%3EA618718567%3C/gale_doaj_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2341479382&rft_id=info:pmid/31931837&rft_galeid=A618718567&rft_doaj_id=oai_doaj_org_article_58cef0b33db54ba99a37cd03be47e14c&rfr_iscdi=true |