Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains
Deoxynivalenol (DON) is one of the most important fungal mycotoxins excreted by different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems. This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs)...
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| Veröffentlicht in: | Journal of AOAC International 2018-09, Vol.101 (5), p.1534-1541 |
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| creator | El-Naggar, Medhat A Alrajhi, Aisha M Fouda, Moustafa M Abdelkareem, Eman M Thabit, Tamer M Bouqellah, Nahla A |
| description | Deoxynivalenol (DON) is one of the most important fungal mycotoxins excreted by different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems.
This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains.
Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates.
Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm.
Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins. |
| doi_str_mv | 10.5740/jaoacint.17-0442 |
| format | Article |
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This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains.
Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates.
Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm.
Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.5740/jaoacint.17-0442</identifier><identifier>PMID: 29724266</identifier><language>eng</language><publisher>United States: Oxford University Press</publisher><subject>Antifungal Agents - chemistry ; Antifungal Agents - pharmacology ; Chemical properties ; Control ; Edible Grain - microbiology ; Fusariosis - microbiology ; Fusariosis - prevention & control ; Fusarium ; Fusarium - drug effects ; Fusarium - growth & development ; Fusarium - metabolism ; Grains ; Hordeum - microbiology ; Humans ; Metal Nanoparticles - chemistry ; Mycotoxins ; Nanoparticles ; Silver ; Silver - chemistry ; Silver - pharmacology ; Trichothecenes - metabolism ; Triticum - microbiology ; Zea mays - microbiology</subject><ispartof>Journal of AOAC International, 2018-09, Vol.101 (5), p.1534-1541</ispartof><rights>COPYRIGHT 2018 Oxford University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-13fe0b7fd8e0a3bbfff8227798a4266d40051724ffdc943cd454b4c59f3179f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29724266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Naggar, Medhat A</creatorcontrib><creatorcontrib>Alrajhi, Aisha M</creatorcontrib><creatorcontrib>Fouda, Moustafa M</creatorcontrib><creatorcontrib>Abdelkareem, Eman M</creatorcontrib><creatorcontrib>Thabit, Tamer M</creatorcontrib><creatorcontrib>Bouqellah, Nahla A</creatorcontrib><title>Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>Deoxynivalenol (DON) is one of the most important fungal mycotoxins excreted by different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems.
This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains.
Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates.
Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm.
Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins.</description><subject>Antifungal Agents - chemistry</subject><subject>Antifungal Agents - pharmacology</subject><subject>Chemical properties</subject><subject>Control</subject><subject>Edible Grain - microbiology</subject><subject>Fusariosis - microbiology</subject><subject>Fusariosis - prevention & control</subject><subject>Fusarium</subject><subject>Fusarium - drug effects</subject><subject>Fusarium - growth & development</subject><subject>Fusarium - metabolism</subject><subject>Grains</subject><subject>Hordeum - microbiology</subject><subject>Humans</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Mycotoxins</subject><subject>Nanoparticles</subject><subject>Silver</subject><subject>Silver - chemistry</subject><subject>Silver - pharmacology</subject><subject>Trichothecenes - metabolism</subject><subject>Triticum - microbiology</subject><subject>Zea mays - microbiology</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1rAjEQhkNpqdb23lPJH1ibrzXuUazagrQHvS_Z7EQiu8mSrKL_vhFrGZgZBp7h5UHolZJxLgV53yuvtHX9mMqMCMHu0JAWQmSyYOw-7WRCMs4kHaCnGPeECDoh7BENWCGZYJPJEMHCGNA99gZvbHOEgL-V850KvdUNROwd3vqT3YGzGi8PUQV7aHHsujFWrsYf4E9nZ4-qAecbvAEdoLcJsg5vfAt4FZR18Rk9GNVEePmbI7RdLrbzz2z9s_qaz9aZ5nzaZ5QbIJU09RSI4lVljJkyJmUxVZe0tSAkpym5MbUuBNe1yEUldF4YTmVqIzS-vt2lPKV1xvdB6VQ1tFZ7B8am-yzPeUHy9DgB5Aro4GMMYMou2FaFc0lJeTFc3gyXVJYXwwl5uyLdoWqh_gduSvkvIE558g</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>El-Naggar, Medhat A</creator><creator>Alrajhi, Aisha M</creator><creator>Fouda, Moustafa M</creator><creator>Abdelkareem, Eman M</creator><creator>Thabit, Tamer M</creator><creator>Bouqellah, Nahla A</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20180901</creationdate><title>Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains</title><author>El-Naggar, Medhat A ; Alrajhi, Aisha M ; Fouda, Moustafa M ; Abdelkareem, Eman M ; Thabit, Tamer M ; Bouqellah, Nahla A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-13fe0b7fd8e0a3bbfff8227798a4266d40051724ffdc943cd454b4c59f3179f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Antifungal Agents - chemistry</topic><topic>Antifungal Agents - pharmacology</topic><topic>Chemical properties</topic><topic>Control</topic><topic>Edible Grain - microbiology</topic><topic>Fusariosis - microbiology</topic><topic>Fusariosis - prevention & control</topic><topic>Fusarium</topic><topic>Fusarium - drug effects</topic><topic>Fusarium - growth & development</topic><topic>Fusarium - metabolism</topic><topic>Grains</topic><topic>Hordeum - microbiology</topic><topic>Humans</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Mycotoxins</topic><topic>Nanoparticles</topic><topic>Silver</topic><topic>Silver - chemistry</topic><topic>Silver - pharmacology</topic><topic>Trichothecenes - metabolism</topic><topic>Triticum - microbiology</topic><topic>Zea mays - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Naggar, Medhat A</creatorcontrib><creatorcontrib>Alrajhi, Aisha M</creatorcontrib><creatorcontrib>Fouda, Moustafa M</creatorcontrib><creatorcontrib>Abdelkareem, Eman M</creatorcontrib><creatorcontrib>Thabit, Tamer M</creatorcontrib><creatorcontrib>Bouqellah, Nahla A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of AOAC International</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Naggar, Medhat A</au><au>Alrajhi, Aisha M</au><au>Fouda, Moustafa M</au><au>Abdelkareem, Eman M</au><au>Thabit, Tamer M</au><au>Bouqellah, Nahla A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains</atitle><jtitle>Journal of AOAC International</jtitle><addtitle>J AOAC Int</addtitle><date>2018-09-01</date><risdate>2018</risdate><volume>101</volume><issue>5</issue><spage>1534</spage><epage>1541</epage><pages>1534-1541</pages><issn>1060-3271</issn><eissn>1944-7922</eissn><abstract>Deoxynivalenol (DON) is one of the most important fungal mycotoxins excreted by different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems.
This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains.
Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates.
Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm.
Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins.</abstract><cop>United States</cop><pub>Oxford University Press</pub><pmid>29724266</pmid><doi>10.5740/jaoacint.17-0442</doi><tpages>8</tpages></addata></record> |
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| subjects | Antifungal Agents - chemistry Antifungal Agents - pharmacology Chemical properties Control Edible Grain - microbiology Fusariosis - microbiology Fusariosis - prevention & control Fusarium Fusarium - drug effects Fusarium - growth & development Fusarium - metabolism Grains Hordeum - microbiology Humans Metal Nanoparticles - chemistry Mycotoxins Nanoparticles Silver Silver - chemistry Silver - pharmacology Trichothecenes - metabolism Triticum - microbiology Zea mays - microbiology |
| title | Effect of Silver Nanoparticles on Toxigenic Fusarium spp. and Deoxynivalenol Secretion in Some Grains |
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