Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro

Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro

Reports indicate that statins (cholesterol-lowering drugs), in addition to lowering cholesterol, have an immunomodulatory effect. This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effe...

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Veröffentlicht in:International journal of morphology 2017-06, Vol.35 (2), p.394
Hauptverfasser: Rosenberg, David R, Kernitsky, Jeremy R, Andrade, Catherine X, Ramirez, Valeria, Violant, Deborah, Nart, Jose
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Kernitsky, Jeremy R
Andrade, Catherine X
Ramirez, Valeria
Violant, Deborah
Nart, Jose
description Reports indicate that statins (cholesterol-lowering drugs), in addition to lowering cholesterol, have an immunomodulatory effect. This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effect of an atorvastatin-medicated dentifrice on CD4+ T cell proliferation. CD4+ T cell proliferation assays and peripheral blood mononuclear cell (PBMC) viability assays were conducted on PBMCs from healthy donors cultured under the following conditions: control, atorvastatin solution, atorvastatin-medicated dentifrice, and dentifrice without atorvastatin at concentrations of 1, 5, 10, 50 and 100 [micro]M. A Generalized Equation Estimation (GEE) model was used to analyze concentration versus proliferation and concentration versus percentage of dead cells within each group evaluated. Atorvastatin-medicated dentifrice (p-value
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This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effect of an atorvastatin-medicated dentifrice on CD4+ T cell proliferation. CD4+ T cell proliferation assays and peripheral blood mononuclear cell (PBMC) viability assays were conducted on PBMCs from healthy donors cultured under the following conditions: control, atorvastatin solution, atorvastatin-medicated dentifrice, and dentifrice without atorvastatin at concentrations of 1, 5, 10, 50 and 100 [micro]M. A Generalized Equation Estimation (GEE) model was used to analyze concentration versus proliferation and concentration versus percentage of dead cells within each group evaluated. Atorvastatin-medicated dentifrice (p-value &lt;0.0001) and atorvastatin solution (p-value &lt;0.0001) significantly inhibited CD4+ T cell proliferation in a dose-dependent manner compared with the dentifrice without atorvastatin and control conditions. Only the relationship between atorvastatin solution and percentage of dead cells was significant compared to the other conditions (p-value 0.019). The results revealed that atorvastatin-medicated dentifrice at concentrations of 1 to 100 [micro]M had immunomodulatory effects, inhibiting CD4+ T cell proliferation without affecting PBMC viability. The other components of the dentifrice did not affect CD4+ T cell proliferation or cell viability, indicating its utility as a vehicle to achieve the desired effects of atorvastatin in periodontal tissue. Controlled clinical trials are still needed to evaluate the clinical effects of an atorvastatin-medicated dentifrice on the periodontium. KEY WORDS: Atorvastatin calcium; Dentifrices; T cells; Flow cytometry. La literatura indica que las estatinas (medicamentos para bajar el colesterol), ademas de reducir el colesterol, tienen un efecto inmunomodulador. Este efecto puede ser beneficioso para el tratamiento de varias enfermedades, incluyendo la enfermedad periodontal. El objetivo de este estudio es evaluar el efecto inmunomodulador de una pasta dental medicada con atorvastatina sobre la proliferacion celular de linfocitos T CD4+. A partir de celulas mononucleares de sangre periferica de donantes sanos (PBMC), se realizaron ensayos de proliferacion y viabilidad de linfocitos T CD4+ bajo las siguientes condiciones: control, solucion de atorvastatina, dentifrico medicado con atorvastatina y dentifrico sin atorvastatina, en concentraciones 1, 5, 10, 50 and 100 [micro]M. Se realizo el analisis estadistico utilizando el modelo Generalized Equation Estimation (GEE) a fin de analizar la concentracion versus la proliferacion y la concentracion versus el porcentaje de muerte celular para cada uno de los grupos. El dentifrico medicado con atorvastatina (valor p &lt;0,0001) y solucion de atorvastatina (valor p &lt;0,0001) inhibieron significativamente la proliferacion de celulas T CD4 + de una manera dependiente de la dosis en comparacion con el dentifrico sin atorvastatina y condiciones de control. Solo la relacion entre la atorvastatina solucion y el porcentaje de celulas muertas fue significativa en comparacion con las otras condiciones (vale-p 0,019). Los resultados revelaron que el dentifrico medicado con atorvastatina en concentraciones de 1 a 100 mM tenia efectos inmunomoduladores, inhibiendo la proliferacion de celulas T CD4 + sin afectar la viabilidad de PBMC. Los otros componentes del dentifrico no afectaron la proliferacion de celulas T CD4 + o la viabilidad celular, indicando su utilidad como vehiculo para conseguir los efectos deseados de atorvastatina en el tejido periodontal. Todavia se necesitan ensayos clinicos controlados para evaluar los efectos clinicos de un dentifrico medicado con atorvastatina sobre el periodonto. PALABRAS CLAVE: Atorvastatina calcica; Dentifricos. Celulas T.; Citometria de flujo.</description><identifier>ISSN: 0717-9367</identifier><language>spa</language><publisher>Universidad de La Frontera, Facultad de Medicina</publisher><subject>Analysis ; Atorvastatin ; Medical schools ; T cells</subject><ispartof>International journal of morphology, 2017-06, Vol.35 (2), p.394</ispartof><rights>COPYRIGHT 2017 Universidad de La Frontera, Facultad de Medicina</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Rosenberg, David R</creatorcontrib><creatorcontrib>Kernitsky, Jeremy R</creatorcontrib><creatorcontrib>Andrade, Catherine X</creatorcontrib><creatorcontrib>Ramirez, Valeria</creatorcontrib><creatorcontrib>Violant, Deborah</creatorcontrib><creatorcontrib>Nart, Jose</creatorcontrib><title>Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro</title><title>International journal of morphology</title><description>Reports indicate that statins (cholesterol-lowering drugs), in addition to lowering cholesterol, have an immunomodulatory effect. This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effect of an atorvastatin-medicated dentifrice on CD4+ T cell proliferation. CD4+ T cell proliferation assays and peripheral blood mononuclear cell (PBMC) viability assays were conducted on PBMCs from healthy donors cultured under the following conditions: control, atorvastatin solution, atorvastatin-medicated dentifrice, and dentifrice without atorvastatin at concentrations of 1, 5, 10, 50 and 100 [micro]M. A Generalized Equation Estimation (GEE) model was used to analyze concentration versus proliferation and concentration versus percentage of dead cells within each group evaluated. Atorvastatin-medicated dentifrice (p-value &lt;0.0001) and atorvastatin solution (p-value &lt;0.0001) significantly inhibited CD4+ T cell proliferation in a dose-dependent manner compared with the dentifrice without atorvastatin and control conditions. Only the relationship between atorvastatin solution and percentage of dead cells was significant compared to the other conditions (p-value 0.019). The results revealed that atorvastatin-medicated dentifrice at concentrations of 1 to 100 [micro]M had immunomodulatory effects, inhibiting CD4+ T cell proliferation without affecting PBMC viability. The other components of the dentifrice did not affect CD4+ T cell proliferation or cell viability, indicating its utility as a vehicle to achieve the desired effects of atorvastatin in periodontal tissue. Controlled clinical trials are still needed to evaluate the clinical effects of an atorvastatin-medicated dentifrice on the periodontium. KEY WORDS: Atorvastatin calcium; Dentifrices; T cells; Flow cytometry. La literatura indica que las estatinas (medicamentos para bajar el colesterol), ademas de reducir el colesterol, tienen un efecto inmunomodulador. Este efecto puede ser beneficioso para el tratamiento de varias enfermedades, incluyendo la enfermedad periodontal. El objetivo de este estudio es evaluar el efecto inmunomodulador de una pasta dental medicada con atorvastatina sobre la proliferacion celular de linfocitos T CD4+. A partir de celulas mononucleares de sangre periferica de donantes sanos (PBMC), se realizaron ensayos de proliferacion y viabilidad de linfocitos T CD4+ bajo las siguientes condiciones: control, solucion de atorvastatina, dentifrico medicado con atorvastatina y dentifrico sin atorvastatina, en concentraciones 1, 5, 10, 50 and 100 [micro]M. Se realizo el analisis estadistico utilizando el modelo Generalized Equation Estimation (GEE) a fin de analizar la concentracion versus la proliferacion y la concentracion versus el porcentaje de muerte celular para cada uno de los grupos. El dentifrico medicado con atorvastatina (valor p &lt;0,0001) y solucion de atorvastatina (valor p &lt;0,0001) inhibieron significativamente la proliferacion de celulas T CD4 + de una manera dependiente de la dosis en comparacion con el dentifrico sin atorvastatina y condiciones de control. Solo la relacion entre la atorvastatina solucion y el porcentaje de celulas muertas fue significativa en comparacion con las otras condiciones (vale-p 0,019). Los resultados revelaron que el dentifrico medicado con atorvastatina en concentraciones de 1 a 100 mM tenia efectos inmunomoduladores, inhibiendo la proliferacion de celulas T CD4 + sin afectar la viabilidad de PBMC. Los otros componentes del dentifrico no afectaron la proliferacion de celulas T CD4 + o la viabilidad celular, indicando su utilidad como vehiculo para conseguir los efectos deseados de atorvastatina en el tejido periodontal. Todavia se necesitan ensayos clinicos controlados para evaluar los efectos clinicos de un dentifrico medicado con atorvastatina sobre el periodonto. PALABRAS CLAVE: Atorvastatina calcica; Dentifricos. Celulas T.; Citometria de flujo.</description><subject>Analysis</subject><subject>Atorvastatin</subject><subject>Medical schools</subject><subject>T cells</subject><issn>0717-9367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqVjk1LA0EMhvegYP34D7nL6tbtutpb2bboQVhoPUvcydTIdAZm0oX-aX-DqS3Wq-QQCG_e5znJBkU9rPPH8r4-y85T-iyKu6ouqkH2NZEQe0yCwj5_IcMdChmYkhe2kTuCBa88W717cVt49h_8zpKgmY6uYQkNOQdtDI4tRS0JfqwZ6FligJZdEFjIxmxvod1RforRwZ5kELrg4a8D7gkEhmAe4hqPfOEeweGR1iltl1OHjcO0s1GpMbx6mCWF8sEg_BpdZqcWXaKrw77IbuazZfOUr9DRG3sbRGt1DK1Z1ciy3idVOXoodYblvx--Af-hfpE</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Rosenberg, David R</creator><creator>Kernitsky, Jeremy R</creator><creator>Andrade, Catherine X</creator><creator>Ramirez, Valeria</creator><creator>Violant, Deborah</creator><creator>Nart, Jose</creator><general>Universidad de La Frontera, Facultad de Medicina</general><scope/></search><sort><creationdate>20170601</creationdate><title>Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro</title><author>Rosenberg, David R ; Kernitsky, Jeremy R ; Andrade, Catherine X ; Ramirez, Valeria ; Violant, Deborah ; Nart, Jose</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-gale_infotracacademiconefile_A5348383813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2017</creationdate><topic>Analysis</topic><topic>Atorvastatin</topic><topic>Medical schools</topic><topic>T cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosenberg, David R</creatorcontrib><creatorcontrib>Kernitsky, Jeremy R</creatorcontrib><creatorcontrib>Andrade, Catherine X</creatorcontrib><creatorcontrib>Ramirez, Valeria</creatorcontrib><creatorcontrib>Violant, Deborah</creatorcontrib><creatorcontrib>Nart, Jose</creatorcontrib><jtitle>International journal of morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosenberg, David R</au><au>Kernitsky, Jeremy R</au><au>Andrade, Catherine X</au><au>Ramirez, Valeria</au><au>Violant, Deborah</au><au>Nart, Jose</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro</atitle><jtitle>International journal of morphology</jtitle><date>2017-06-01</date><risdate>2017</risdate><volume>35</volume><issue>2</issue><spage>394</spage><pages>394-</pages><issn>0717-9367</issn><abstract>Reports indicate that statins (cholesterol-lowering drugs), in addition to lowering cholesterol, have an immunomodulatory effect. This effect may be beneficial for the treatment of several diseases, including periodontal disease. The aim of the present study was to evaluate the immunomodulatory effect of an atorvastatin-medicated dentifrice on CD4+ T cell proliferation. CD4+ T cell proliferation assays and peripheral blood mononuclear cell (PBMC) viability assays were conducted on PBMCs from healthy donors cultured under the following conditions: control, atorvastatin solution, atorvastatin-medicated dentifrice, and dentifrice without atorvastatin at concentrations of 1, 5, 10, 50 and 100 [micro]M. A Generalized Equation Estimation (GEE) model was used to analyze concentration versus proliferation and concentration versus percentage of dead cells within each group evaluated. Atorvastatin-medicated dentifrice (p-value &lt;0.0001) and atorvastatin solution (p-value &lt;0.0001) significantly inhibited CD4+ T cell proliferation in a dose-dependent manner compared with the dentifrice without atorvastatin and control conditions. Only the relationship between atorvastatin solution and percentage of dead cells was significant compared to the other conditions (p-value 0.019). The results revealed that atorvastatin-medicated dentifrice at concentrations of 1 to 100 [micro]M had immunomodulatory effects, inhibiting CD4+ T cell proliferation without affecting PBMC viability. The other components of the dentifrice did not affect CD4+ T cell proliferation or cell viability, indicating its utility as a vehicle to achieve the desired effects of atorvastatin in periodontal tissue. Controlled clinical trials are still needed to evaluate the clinical effects of an atorvastatin-medicated dentifrice on the periodontium. KEY WORDS: Atorvastatin calcium; Dentifrices; T cells; Flow cytometry. La literatura indica que las estatinas (medicamentos para bajar el colesterol), ademas de reducir el colesterol, tienen un efecto inmunomodulador. Este efecto puede ser beneficioso para el tratamiento de varias enfermedades, incluyendo la enfermedad periodontal. El objetivo de este estudio es evaluar el efecto inmunomodulador de una pasta dental medicada con atorvastatina sobre la proliferacion celular de linfocitos T CD4+. A partir de celulas mononucleares de sangre periferica de donantes sanos (PBMC), se realizaron ensayos de proliferacion y viabilidad de linfocitos T CD4+ bajo las siguientes condiciones: control, solucion de atorvastatina, dentifrico medicado con atorvastatina y dentifrico sin atorvastatina, en concentraciones 1, 5, 10, 50 and 100 [micro]M. Se realizo el analisis estadistico utilizando el modelo Generalized Equation Estimation (GEE) a fin de analizar la concentracion versus la proliferacion y la concentracion versus el porcentaje de muerte celular para cada uno de los grupos. El dentifrico medicado con atorvastatina (valor p &lt;0,0001) y solucion de atorvastatina (valor p &lt;0,0001) inhibieron significativamente la proliferacion de celulas T CD4 + de una manera dependiente de la dosis en comparacion con el dentifrico sin atorvastatina y condiciones de control. Solo la relacion entre la atorvastatina solucion y el porcentaje de celulas muertas fue significativa en comparacion con las otras condiciones (vale-p 0,019). Los resultados revelaron que el dentifrico medicado con atorvastatina en concentraciones de 1 a 100 mM tenia efectos inmunomoduladores, inhibiendo la proliferacion de celulas T CD4 + sin afectar la viabilidad de PBMC. Los otros componentes del dentifrico no afectaron la proliferacion de celulas T CD4 + o la viabilidad celular, indicando su utilidad como vehiculo para conseguir los efectos deseados de atorvastatina en el tejido periodontal. Todavia se necesitan ensayos clinicos controlados para evaluar los efectos clinicos de un dentifrico medicado con atorvastatina sobre el periodonto. PALABRAS CLAVE: Atorvastatina calcica; Dentifricos. Celulas T.; Citometria de flujo.</abstract><pub>Universidad de La Frontera, Facultad de Medicina</pub></addata></record>
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subjects Analysis
Atorvastatin
Medical schools
T cells
title Atorvastatin-Medicated Dentifrice Significantly Inhibits CD4+ T Cell Proliferation: In vitro Pilot Study/ Pasta Dental Medicada con Atorvastatina Inhibe de Forma Significativa la Proliferacion de Celulas T CD4+: Un Estudio Piloto In vitro
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