Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas

Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas

Several studies indicated that pancreatic [beta]-cells death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the [beta]-cellss in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randoml...

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Veröffentlicht in:International journal of morphology 2017-03, Vol.35 (1), p.16
Hauptverfasser: Nazari, Zahra, Nabiuni, Mohammad, Ghaffari, Soraya, Saeidi, Mohsen, Shahriyari, Alireza, Golalipour, Mohammad Jafar
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Apoptosis
Gestational diabetes
Pancreatic beta cells
Streptozocin
Type 2 diabetes
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container_start_page 16
container_title International journal of morphology
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creator Nazari, Zahra
Nabiuni, Mohammad
Ghaffari, Soraya
Saeidi, Mohsen
Shahriyari, Alireza
Golalipour, Mohammad Jafar
description Several studies indicated that pancreatic [beta]-cells death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the [beta]-cellss in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of [beta]-cellss and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in [beta]-cells number in offspring of GDM (p
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This experimental study was designed to determine the effect of gestational diabetes on the [beta]-cellss in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of [beta]-cellss and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in [beta]-cells number in offspring of GDM (p&lt;0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P&lt;0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring. KEY WORDS: Gestational diabetes mellitus; Pancreatic islets (Langerhans); [beta]-cells; Apoptosis; Rat offspring. Varios estudios indican que la muerte de las celulas b del pancreas se produce tanto en la diabetes Tipo 1 como en la Tipo 2. Este estudio experimental fue disenado para determinar el efecto de la diabetes gestacional en las celulas b del pancreas en crias de ratas de 16 semanas. Para ello, ratas Wistar adultas de entre 10-12 semanas fueron asignadas al azar en dos grupos: control y diabetes. El grupo diabetes recibio 40 mg / kg / peso corporal de estreptozotocina (STZ) en el dia cero de la gestacion. Despues del parto, a las 16 semanas, las crias de las madres diabeticas y controles de madres con diabetes gestacional (MDG), fueron sacrificadas para la extraccion del pancreas, el cual posteriormente fue fijado. Se conto el numero de celulas b del pancreas mediante tincion con el metodo de Gomori. Ademas, se detecto apoptosis en el tejido del pancreas de la descendencia diabetica y el grupo control mediante un ensayo TUNEL. Los resultados mostraron una reduccion significativa en el numero de celulas b en la descendencia de MDG (p &lt;0,05). El ensayo TUNEL mostro que el numero de celulas apoptoticas aumento en MDG en comparacion con los controles (P &lt;0,05). Este estudio revelo que la diabetes gestacional induce apoptosis de celulas b en el pancreas de crias de ratas de 16 semanas. PALABRAS CLAVE: Diabetes mellitus gestacional; Islotes pancreaticos; Celulas b; Descendientes; Apoptosis; Ratas.</description><identifier>ISSN: 0717-9367</identifier><language>spa</language><publisher>Universidad de La Frontera, Facultad de Medicina</publisher><subject>Apoptosis ; Gestational diabetes ; Pancreatic beta cells ; Streptozocin ; Type 2 diabetes</subject><ispartof>International journal of morphology, 2017-03, Vol.35 (1), p.16</ispartof><rights>COPYRIGHT 2017 Universidad de La Frontera, Facultad de Medicina</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Nazari, Zahra</creatorcontrib><creatorcontrib>Nabiuni, Mohammad</creatorcontrib><creatorcontrib>Ghaffari, Soraya</creatorcontrib><creatorcontrib>Saeidi, Mohsen</creatorcontrib><creatorcontrib>Shahriyari, Alireza</creatorcontrib><creatorcontrib>Golalipour, Mohammad Jafar</creatorcontrib><title>Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas</title><title>International journal of morphology</title><description>Several studies indicated that pancreatic [beta]-cells death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the [beta]-cellss in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of [beta]-cellss and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in [beta]-cells number in offspring of GDM (p&lt;0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P&lt;0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring. KEY WORDS: Gestational diabetes mellitus; Pancreatic islets (Langerhans); [beta]-cells; Apoptosis; Rat offspring. Varios estudios indican que la muerte de las celulas b del pancreas se produce tanto en la diabetes Tipo 1 como en la Tipo 2. Este estudio experimental fue disenado para determinar el efecto de la diabetes gestacional en las celulas b del pancreas en crias de ratas de 16 semanas. Para ello, ratas Wistar adultas de entre 10-12 semanas fueron asignadas al azar en dos grupos: control y diabetes. El grupo diabetes recibio 40 mg / kg / peso corporal de estreptozotocina (STZ) en el dia cero de la gestacion. Despues del parto, a las 16 semanas, las crias de las madres diabeticas y controles de madres con diabetes gestacional (MDG), fueron sacrificadas para la extraccion del pancreas, el cual posteriormente fue fijado. Se conto el numero de celulas b del pancreas mediante tincion con el metodo de Gomori. Ademas, se detecto apoptosis en el tejido del pancreas de la descendencia diabetica y el grupo control mediante un ensayo TUNEL. Los resultados mostraron una reduccion significativa en el numero de celulas b en la descendencia de MDG (p &lt;0,05). El ensayo TUNEL mostro que el numero de celulas apoptoticas aumento en MDG en comparacion con los controles (P &lt;0,05). Este estudio revelo que la diabetes gestacional induce apoptosis de celulas b en el pancreas de crias de ratas de 16 semanas. PALABRAS CLAVE: Diabetes mellitus gestacional; Islotes pancreaticos; Celulas b; Descendientes; Apoptosis; Ratas.</description><subject>Apoptosis</subject><subject>Gestational diabetes</subject><subject>Pancreatic beta cells</subject><subject>Streptozocin</subject><subject>Type 2 diabetes</subject><issn>0717-9367</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqVjU1uwjAQhb2gErRwh7lA2hQTAsuQ_q6KEHs0OGM0yNhRxrlXj1grtFW31Sze_L3vjdQkLx_LbK2X5VjdipzzfF6UeTFRn68kESMHjw6eGI8USeDdN71JukVvOkpnAxuKmNXknEDVhjYGYQH2UDW9i7DDCB_WStuxPz38cga4ucKvzD_mhiDxeocywNPsfgIFyEPdMQ5fCZ6aIQhlqm4sOqHZt96p-5fnff2WndDRgb0NsUOTqqELm-DJctpXhV6s9ErPF_rfhi_htmj-</recordid><startdate>20170301</startdate><enddate>20170301</enddate><creator>Nazari, Zahra</creator><creator>Nabiuni, Mohammad</creator><creator>Ghaffari, Soraya</creator><creator>Saeidi, Mohsen</creator><creator>Shahriyari, Alireza</creator><creator>Golalipour, Mohammad Jafar</creator><general>Universidad de La Frontera, Facultad de Medicina</general><scope/></search><sort><creationdate>20170301</creationdate><title>Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas</title><author>Nazari, Zahra ; Nabiuni, Mohammad ; Ghaffari, Soraya ; Saeidi, Mohsen ; Shahriyari, Alireza ; Golalipour, Mohammad Jafar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-gale_infotracacademiconefile_A5348383243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>spa</language><creationdate>2017</creationdate><topic>Apoptosis</topic><topic>Gestational diabetes</topic><topic>Pancreatic beta cells</topic><topic>Streptozocin</topic><topic>Type 2 diabetes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nazari, Zahra</creatorcontrib><creatorcontrib>Nabiuni, Mohammad</creatorcontrib><creatorcontrib>Ghaffari, Soraya</creatorcontrib><creatorcontrib>Saeidi, Mohsen</creatorcontrib><creatorcontrib>Shahriyari, Alireza</creatorcontrib><creatorcontrib>Golalipour, Mohammad Jafar</creatorcontrib><jtitle>International journal of morphology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nazari, Zahra</au><au>Nabiuni, Mohammad</au><au>Ghaffari, Soraya</au><au>Saeidi, Mohsen</au><au>Shahriyari, Alireza</au><au>Golalipour, Mohammad Jafar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas</atitle><jtitle>International journal of morphology</jtitle><date>2017-03-01</date><risdate>2017</risdate><volume>35</volume><issue>1</issue><spage>16</spage><pages>16-</pages><issn>0717-9367</issn><abstract>Several studies indicated that pancreatic [beta]-cells death occurs in both type 1 and type 2 diabetes. This experimental study was designed to determine the effect of gestational diabetes on the [beta]-cellss in 16-week-old rat offspring. By this aim, adult Wistar rats aged 10-12 weeks were randomly allocated in control and diabetic groups. The diabetic group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic offspring of GDM mothers and controls at the age of 16 weeks were sacrificed and pancreases harvested and fixed. The number of [beta]-cellss and were counted by Gomori's method staining. Also, apoptosis in pancreas tissue of diabetic and control offspring was detected by TUNEL assay. Results showed a significant reduction in [beta]-cells number in offspring of GDM (p&lt;0.05). TUNEL assay showed that the number of apoptotic cells increased in GDM compared to controls (P&lt;0.05). This study revealed that gestational diabetes induces pancreatic beta-cells apoptosis in 16-week-old rat offspring. KEY WORDS: Gestational diabetes mellitus; Pancreatic islets (Langerhans); [beta]-cells; Apoptosis; Rat offspring. Varios estudios indican que la muerte de las celulas b del pancreas se produce tanto en la diabetes Tipo 1 como en la Tipo 2. Este estudio experimental fue disenado para determinar el efecto de la diabetes gestacional en las celulas b del pancreas en crias de ratas de 16 semanas. Para ello, ratas Wistar adultas de entre 10-12 semanas fueron asignadas al azar en dos grupos: control y diabetes. El grupo diabetes recibio 40 mg / kg / peso corporal de estreptozotocina (STZ) en el dia cero de la gestacion. Despues del parto, a las 16 semanas, las crias de las madres diabeticas y controles de madres con diabetes gestacional (MDG), fueron sacrificadas para la extraccion del pancreas, el cual posteriormente fue fijado. Se conto el numero de celulas b del pancreas mediante tincion con el metodo de Gomori. Ademas, se detecto apoptosis en el tejido del pancreas de la descendencia diabetica y el grupo control mediante un ensayo TUNEL. Los resultados mostraron una reduccion significativa en el numero de celulas b en la descendencia de MDG (p &lt;0,05). El ensayo TUNEL mostro que el numero de celulas apoptoticas aumento en MDG en comparacion con los controles (P &lt;0,05). Este estudio revelo que la diabetes gestacional induce apoptosis de celulas b en el pancreas de crias de ratas de 16 semanas. PALABRAS CLAVE: Diabetes mellitus gestacional; Islotes pancreaticos; Celulas b; Descendientes; Apoptosis; Ratas.</abstract><pub>Universidad de La Frontera, Facultad de Medicina</pub></addata></record>
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subjects Apoptosis
Gestational diabetes
Pancreatic beta cells
Streptozocin
Type 2 diabetes
title Gestational Diabetes Induces Pancreatic Beta-Cells Apoptosis in Adult Rat Offspring/Diabetes Gestacional Induce Apoptosis de Celulas Beta del Pancreas en Crias de Ratas Adultas
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